Wyniki wyszukiwania

1

An efficient method of genomic DNA isolation from plant tissues

100%

Strozycki P. M., Legocki A. B.

Acta Biochimica Polonica

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1995

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tom 42

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nr 3

The manuscript describes an easy method of isolation of plant genomic DNA. This method allowed us to isolate substantial amounts of good quality DNA from lupin (Lupinus luteus) tissues. The described method also appeared to be useful for genomic DNA isolation from tissues of other plants.

2

Rapid DNA extraction for screening soil filamentous fungi using PCR amplification

100%

Plaza G. A., Upchurch R., Brigmon R. L., Whitman W. B., Ulfig K.

Polish Journal of Environmental Studies

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2004

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tom 13

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nr 3

A simple and rapid procedure for efficiently isolating fungi DNA suitable for use as a template for PCR amplification and other molecular assays is described. The main advantages of the method are: (1) the mycelium is directly recovered from Petri-dish cultures; (2) the technique is rapid and relatively easy to perform , and (3) it allows for processing of around 50 samples during a single day; (4) it is inexpensive; (5) the quality and quantity of DNA obtained are suitable for molecular assays; (6) it can be applied to filamentous fungi from soil as well as from a fungi from other environmental sources; and (7) it does not require the use of expensive and specialized equipment or hazardous reagents.

3

Methods of DNA isolation from poultry meal

88%

Natonek-Wisniewska M., Slota E.

Journal of Animal and Feed Sciences

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2007

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tom 16

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nr 3

490-496

4

The influence of high temperature on the possibility of DNA typing in various human tissues

75%

Maciejewska A., Wlodarczyk R., Pawlowski R.

Folia Histochemica et Cytobiologica

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2015

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tom 53

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nr 4

5

Plant DNA isolation from differently preserved Thalictrum leaf tissues and its use in RAPD analysis

75%

Liber Z., Nikolic T., Mitic B.

Acta Biologica Cracoviensia. Series Botanica

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2002

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tom 44

There are many unsolved taxonomic problems at the intraspecific level in the genus Thalictrum (Ranunculaceae), which may be clarified using molecular systematics methods. No molecular systematics methods have been applied yet to the genus Thalictrum; this study analyzes different leaf tissue preservation and DNA isolation techniques, and the applicability of RAPD. A modified DNA isolation procedure using solution of laundry detergent as a detergent buffer system was the most suitable, especially for dealing with large samples. Since the use of differently preserved leaf tissues simultaneously with fresh leaf tissue may improve sampling in taxonomic research, and because the RAPD technique is sensitive to different factors, the possible drawbacks of using such tissues in RAPD analyses were checked. Of the four preserved leaf tissues, only DNA from silica gel-preserved leaf tissue gave suitably reliable RAPD results to be used with fresh leaf tissue in more extensive taxonomic research. Differently preserved leaf tissues are very problematic starting materials for simultaneous use with fresh leaf tissue in the same RAPD analysis. If differently preserved leaf tissues are to be used and reliable results are to be obtained, research techniques similar to those used in this paper should be applied.

6

Optimization of DNA isolation procedure as the first step in identification of Phytophthora spp.

75%

Wiejacha K., Szkuta G., Orlikowska T.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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2002

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tom 50

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nr 3

7

Application of different DNA isolation and quantification methods in relation to the age of a fungal culture and type of a medium for some Fusarium species

63%

Pszczolkowska A., Olszewski J., Fordonski G., Plodzien K., Lapinski M.

Polish Journal of Natural Sciences

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2004

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tom 16

87-109

8

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents

63%

Oskiera M., Szczech M., Bartoszewski G.

Journal of Horticultural Research

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2015

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tom 23

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nr 1

9

Genetic variation of Aconitum sect. Aconitum (Ranunculaceae) at a macrogeographical scale in the Carpathians

63%

Sutkowska A., Warzecha T., Mitka J.

Polish Journal of Ecology

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2017

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tom 65

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nr 1

The tetraploid (2n = 32) Aconitum sect. Aconitum in the Eastern Carpathians, Southern Carpathians and Apušeni Mts. is represented by high-mountain A. bucovinese, A. firmum subsp. fissurae and their putative taxonomic hybrid A. ×nanum. The aim of the paper was to reveal which delimiting system: taxonomic vs.geographic-population better explains genetic variability (ISSR — Inter Simple Sequence Repeats) of the Aconitum individuals in the Eastern/Southern Carpathians. Twenty nine plants sampled in five populations within entire range of taxa distribution were assigned to genetic groups according to a Bayesian STRUCTURE analysis, neighbour-net classification (NN), and nonmetric multidimensional scaling ordination (NMDS). Three taxa were distributed in four (NN, NMDS) or two (STRUCTURE) genetic groups, and the partitioning of genetic variation with analysis of molecular variance (AMOVA) revealed the highest percentage of variation attributed to the four ISSR genetic groups (22.6%), then to the two STRUCTURE groups (18.9%) and three taxa (15.6%, all P < 0.001), and finally to the three geographic regions (6.5%, P = 0.013). Genetic groups harbored specimens from distant regions: A. f. subsp. fissurae had similar genetic profiles in the Southern Carpathians and Apušeni Mts. (100% support), and some specimens of A. bucovinense had genetic links with A. f. subsp. fissurae. The hybrid species A. ×nanum was genetically specific. We concluded that (i) genetic links between nowadays distantly located populations could have originated in the effect of ancient contacts and hybridization, (ii) probably in the Carpathians two ancient genetic centers of the A. sect. Aconitum existed and (iii) high genetic specificity of the hybrid species A. ×nanum deserves further studies.

10

Ocena wybranych metod izolacji DNA pod wzgledem ich przydatnosci w hodowli pszenicy

63%

Pietrusinska A., Czembor P. C.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2007

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nr 244

59-68

11

An improved method for efficient isolation and purification of genomic DNA from filamentous cyanobacteria belonging to genera Anabaena, Nodularia and Nostoc

63%

Kaczynska A., Los M., Wegrzyn G.

Oceanological and Hydrobiological Studies

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2013

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tom 42

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nr 1

12

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

63%

Nuc K., Nuc P., Pawelkiewicz J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

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1993

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tom 41

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nr 2

13

Polymerase chain reaction for the differentiation of Marek's disease virus strains

63%

Kozdrun W., Samorek-Salamonowicz E., Czekaj H.

Bulletin of the Veterinary Institute in Puławy

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2001

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tom 45

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nr 1

The aim of this study was an attempt to apply PCR for diagnosis of Marek's disease. Eighteen field Marek's disease virus strains and 4 standard strains: virulent HPRS₁₆ strain and attenuated CVI 988 strain (serotype 1 - MDV-1), apathogenic SB 1strain (serotype 2 - MDV-2) and non - pathogenic turkeys HVT FC 126 strain (serotype 3 MDV-3) were used. Chicken anaemia virus (CAV) and infectious laryngotracheitis virus (ILTV) were used as control of PCR. The virus strains were cultured in CEF and total DNA was isolated by A@A Biotechnology kit. Three pairs of primers were used: for gene A of serotype 1, for 132 bp sequence of serotype 1 and for gene A of serotype 3. PCR was carried out under the following conditions: 94°C - 1 min (denaturation), 55°C - 30 s (annealing), 72°C -30 s (elongation). PCR products were analysed by electrophoresis in 2% agarose gel at 100 V/h. The PCR product of 314 bp was obtained from all field strains and HPRS₁₆ standard after the primers for gene A of serotype 1 were used. After the primers for 132 bp sequence of serotype 1 were used, 434 bp fragment for 19 DNA samples derivied from field strains and standard HPRS₁₆ strain and 1033 bp fragment from attenuated CVI 988 strain were obtained. Application of primers for gene A serotype 3 allowed the detection of 436 bp product only from non-pathogenic HVT FC 126 strain (serotype 3). These results indicated that the PCR technique can be used for the differentiation of MDV strains.

14

Molecular studies in osteogenesis imperfecta [OI] II. Evaluation of intragenic polymorphic sites in COL1A1 and COL1A2 loci

63%

Kostyk E., Sucharski P., Pietrzyk J. J., Kruczek A.

Journal of Applied Genetics

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1998

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tom 39

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nr 4

The goal of the study was to evaluate intragenic polymorphic sites in COL1A1 and COL1A2 loci. For COL1A1 the following intragenic markers were used: PCR-RFLP (COL1A1), G/A polymorphism in exon 45 of COL1A1 and C/T polymorphism in +88 position of COL1A1 non-translatable 3’ end. For COL1A2 PCR-VNTR was analyzed. 17 families were examined (6 of the "simplex" type and 11 of the "multiple" type). In 8 out of 11 "multiplex" families the segregation of the markers revealed correlation with OI, whereas the other 3 were non-informative. The method was not useful in "simplex" families.

15

A method of DNA isolation using a commercial washing powder

63%

Brzuzan P.

Polskie Archiwum Hydrobiologii

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1997

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tom 44

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nr 3

16

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

63%

Ferry A. E., Pace B. S.

Cellular and Molecular Biology Letters

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1998

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tom 03

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nr 4

Obtaining adequate samples for nucleic acid or protein analysis from a limited number of cells can be a difficult task. The steps for isolation of DNA, cytoplasmic RNA and nuclear proteins from mononuclear cells collected from a single peripheral blood sample are outlined below. A previously described technique for rapid isolation of nuclear proteins was modified to acquire RNA and DNA of sufficient quantity and quality to perform analyses at the molecular level without altering the quality of protein extracted. This approach is applicable for use with peripheral blood mononuclear cells, primary cultures and immortalized cell lines.

17

of strains isolated in the

63%

Martirosian G., Meisel-Mikolajczyk F., Stanczak J., Flis D.

Acta Microbiologica Polonica

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1995

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tom 44

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nr 1

18

Ocena zroznicowania genetycznego rozanecznikow [Rhododendron sp.] na podstawie markerow RAPD

51%

Czernicka M., Czernicki W., Klein M., Grzebelus D.

Zeszyty Problemowe Postępów Nauk Rolniczych

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2007

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tom 517

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nr 1

225-233

The aim of our study was to evaluate the genetic relationship of Rhododendrons from Rhododendron collection at Tomaszkowice in Pogórze Wielickie. Leaves and leaf buds of three evergreen Asiatic species: R. aureum, R. brachycarpum, R. purdomii, six cultivars from Catawbiense Hybridum group (R. catawbiense): Catharine van Tol, Nova Zembla, Album Novum, Boursoult, Old Port, Hachmann’s Charmant and one cultivar from Yakushimanum Hybridum group: Koichiro Wada were investigated. Two plants from each accession were analysed. DNA was isolated by Ziegenhagen and Scholz [1998] protocol, dedicated to the difficult species in terms of high quality DNA extraction. After isolation, DNA was amplified with 19 RAPD primers. We obtained 255 RAPD markers and worked out a dendrogram, which illustrated the level of genetic diversity among the tested plants. Our study confirmed a close relationship between rhododendrons from Catawbiense Hybridum group. Another group was constituted by R. aureum and R. purdomii. The third group consisted of R. brachycarpum and Koichiro Wada cv.

19

Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture

51%

Szteyn J., Wiszniewska-Laszczych A., Ruszczynska A.

Polish Journal of Veterinary Sciences

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2008

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tom 11

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nr 1

25-28

Detection of Mycobacterium paratuberculosis (MAP) in tissues of patients suffering from Crohn's disease has given rise to speculation that this mycobacterium may play some role in the development of this disease in humans. Food products, especially milk obtained from animals infected with paratuberculosis, may be a potential vector of MAP to humans, yet the detection of this pathogen poses a number of difficulties. This study was aimed at comparing the effectiveness of MAP isolation from milk samples. Mycobacteria were detected by means of two methods: direct isolation of DNA using a QIAamp DNA Mini Kit by Qiagen, and a culture method with the use of HEYM culture medium. Analyses were carried out on 87 samples of udder cow milk originating from a herd that exhibited seropositive and serodoubtful reactions against paratuberculosis. The presence of an insertion sequence IS-900 was detected in 18 samples of udder milk analyzed with the method of direct DNA isolation and in two samples analyzed by means of the culture method.

20

The evaluation of the genetic similarity of roses [Rosa L.] in Caninae section

51%

Nowak R., Polok K.

Seria Biologiczna. Uniwersytet im. Adama Mickiewicza w Poznaniu

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2005

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tom 72

Ograniczanie wyników

An efficient method of genomic DNA isolation from plant tissues

Rapid DNA extraction for screening soil filamentous fungi using PCR amplification

Methods of DNA isolation from poultry meal

The influence of high temperature on the possibility of DNA typing in various human tissues

Plant DNA isolation from differently preserved Thalictrum leaf tissues and its use in RAPD analysis

Optimization of DNA isolation procedure as the first step in identification of Phytophthora spp.

Application of different DNA isolation and quantification methods in relation to the age of a fungal culture and type of a medium for some Fusarium species

Molecular identification of Trichoderma strains collected to develop plant growth-promoting and biocontrol agents

Genetic variation of Aconitum sect. Aconitum (Ranunculaceae) at a macrogeographical scale in the Carpathians

Ocena wybranych metod izolacji DNA pod wzgledem ich przydatnosci w hodowli pszenicy

An improved method for efficient isolation and purification of genomic DNA from filamentous cyanobacteria belonging to genera Anabaena, Nodularia and Nostoc

The nucleotide sequence and organization of nuclear 5S rRNA genes in yellow lupine

Polymerase chain reaction for the differentiation of Marek's disease virus strains

Molecular studies in osteogenesis imperfecta [OI] II. Evaluation of intragenic polymorphic sites in COL1A1 and COL1A2 loci

A method of DNA isolation using a commercial washing powder

Multiple extraction method for DNA, RNA and nuclear protein isolation from peripheral blood

of strains isolated in the

Ocena zroznicowania genetycznego rozanecznikow [Rhododendron sp.] na podstawie markerow RAPD

Effectiveness of Mycobacterium paratuberculosis isolation from raw milk by means of direct isolation of DNA and classic culture

The evaluation of the genetic similarity of roses [Rosa L.] in Caninae section