In the presented investigations contents of selected microelements were determined in soil and meadow sward of static fertilizer experiment at Czarny Potok near Krynica. The experiment set up in 1968 on the natural mountain meadow comprises 8 fertilizer objects. The studies presented in the work concern 36thyear of the experiment. B, Mn, Zn, Fe, Cu and Co were determined in the 0-10 cm soil. Soil microelement forms soluble in 1 mol HCl dm⁻³ and acidification indices were assessed. Long-term mineral fertilization had a significant effect on the contents of microelements in the soil of the permanent meadow and these changes were multidirectional. Diversification in the contents of microelements resulted from unilateral fertilization with phosphorus, acidifying effect of fertilizers and from liming which blocked solubility of microelements in the soil. The contents of most microelements in the soil of mountain meadow fertilized with mineral fertilizers for many years were considered as medium with the exception of boron which content oscillated between the low and medium. This justifies the necessity of boron fertilization.