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The morphogenesis of the digestive tract and swim bladder of ide Leuciscus idus (L.) was analyzed using histopathological methods. At the moment of hatching, ide larvae are more advanced in their development than the larvae of other teleost fish species. Endo-exogenous nutrition began on day 2 post hatch. At this stage of development, the intestine was lined with a single-layered cylindrical epithelium, and both liver and pancreas began to function. Signs of digestion and enterocyte protein and lipid absorption were observed on day 6 of development. The mucus cells in the mouth, esophagus and gullet produced neutral and acidic mucins. Intestinal cuboid cells appeared on the fourth day and only began secreting acidic carboxyl and sulfate mucins. The differentiation of the air duct and the posterior chamber of the swim bladder was observed on the day of hatching, and the bladder inflated after 24 hours. The anterior chamber began to form on day 9, and it inflated with gas between days 23 and 25 of larval development.
Background. Ghrelin, a newly discovered hormone is involved mainly in the regulation of body energy homeostasis. It has also been proved that ghrelin affects many other processes including the control of the hypothalamo-pituitary-gonadal axis in vertebrates. Ghrelin interacts also with other peptides and neurotransmitters, which are involved in gonadotropin release, such as endogenous opioid peptides. The aim of the experiment was to compare the effects of ghrelin and naltrexone, an opioid antagonist, on LH secretion by pituitary cells of mature female and male common carp, Cyprinus carpio L. Materials and methods. In the in vitro experiment the time-dependent (10 and 24 h) action of ghrelin (10-7 or 10-6 M) and naltrexone at 10-6 M added alone or in combination on LH secretion by enzymatically dispersed pituitary cells of mature female and male carp (Cyprinus carpio L.) was studied. Results. Ghrelin alone at a dose of 10-6 M stimulated LH secretion after 10 h of female- but not male-derived cell incubations. Ghrelin at 10-7 M had no effect on LH secretion from either female or male cells. Naltrexone alone stimulated LH secretion only after 24 h of female cell culture. In male cells no significant changes in LH secretion in response to naltrexone alone were found after 10 or 24 h incubation period. Combined treatment (ghrelin 10-7 or 10-6 M and naltrexone) stimulated LH secretion in female and male cell incubations at 10 and 24 h: LH levels were significantly higher in comparison to control, to ghrelin alone (in cells of both sexes) and to naltrexone alone (in male cells only). Conclusion. The results suggest that opioids and ghrelin may control LH secretion in carp acting synergistically, probably through the same receptor type. The concept of opioid and ghrelin interaction in the gonadal steroid feedback on LH release is also discussed.
Background. Fish gut bacteria may be used as probiotics for fish. Those occurring in the gastrointestinal (GI) tract of rohu, Labeo rohita (Hamilton, 1822), have not been sufficiently studied. This study was intended: to detect bacteria in the intestine of rohu by scanning electron microscopy (SEM); to evaluate the existence of heterotrophic, proteolytic, amylolytic, and cellulolytic bacteria in three different regions of the gut of rohu; and to identify more than 25% of the isolated gut bacteria by 16S rRNA. Materials and Methods. The GI tracts were removed and divided into three regions: foregut, midgut, and hindgut. Tissues used for SEM were fixed in glutaraldehyde, washed in heparinised saline, dehydrated in graded ethanol, fixed in amyl acetate, dried with liquid nitrogen, coated with gold, observed, and photographed. Homogenates of the intestinal segments were spread onto tryptic soy agar plates and selected nutrient media plates to determine heterotrophic, proteolytic, amylolytic, and cellulolytic bacterial populations, respectively. Of the 59 adherent bacterial strains isolated from the GI tract of rohu 16 isolates were tried for identification by 16S rRNA genes. Results. Bacteria were observed to adhere to the gut enterocyte surfaces. Population level of enzyme-producing bacteria was higher in the midgut and hindgut regions than that observed in the foregut region. Furthermore, dense amylolytic and cellulolytic bacterial population in comparison to the proteolytic population was noticed in the GI tract of L. rohita. Highest density of the cellulolytic bacterial population in the hindgut region may indicate that fermentative degradation occurs in this part of the GI tract. Eleven adherent bacterial strains belonged to bacilli, 2 strains to Pseudomonas, 1 strain to Aeromonas, 1 strain was most closely related to Enterobacter, while 1 strain showed < 97% 16S rRNA sequence similarity in BLAST program was treated as unknown. Conclusion. Autochthonous bacteria were present in the GI tract of rohu and that the autochthonous microbiota possess enzymatic activity that might be beneficial for the fish.
Background. The omnivorous freshwater loach, Lepidocephalichthys guntea (Hamilton, 1822) inhibiting unique ecological niche where oxygen content is low. Its alimentary canal is remarkably short, which is surprising considering the fact that this fish is an omnivore. This peculiarity can be correlated with the specific nature of food. Therefore, the aim of the present study was to investigate the mucosal adaptation of the intestine in adult L. guntea with respective digestive- as well as respiratory function. Materials and Methods. The surface design of the mucosa of different sections (anterior, mid, and posterior) of the intestine of L. guntea were studied using light and scanning electron microscope following commonly accepted procedures. Results. The mucosa of the anterior intestine was provided with various types of mucosal folds and crowded with almost closely oval or rounded columnar epithelial cells with prominent microridges. The irregular mucosal surface of the mid intestine was exemplified by regularly spaced columnar epithelial cells with minute and prominent microridges/microvilli. On the contrary, irregular mucosal folds with stumpy villi were detected in the posterior intestine. The mucosal surface of that region was made up of columnar epithelial cells containing inconspicuous microridges. In that region the submucosa was provided with increasing number of blood vessels. Various cellular elements in the anterior and mid intestine were accordingly adapted to serve the important functions of absorption and gas exchange. However, the arrangement of low mucosal folds and dense blood capillaries in between the folds in the posterior intestine unequivocally suggested its respiratory role. Conclusion. The micro-architectural pattern and cellular architecture of the anterior intestine and middle intestine of L. guntea unequivocally suggests that these two regions are adapted to serve major role of digestion and absorption process. However, major changes that appear in the posterior intestine are considerably reduction of the absorptive area and the penetration of blood capillaries in between the columnar epithelial cells. Therefore, the posterior intestine of L. guntea is probably adapted to suit its role for aerial respiration. However, further investigation is needed the detailed physiological processes involved for aerial respiration in the region concerned.
Background. Obtaining the appropriate quantity of milt and spermatozoa of biologically good quality depends on a number of environmental factors. Additional factors may be involved while using a hormonal stimulation. The aim of this study was to analyse the effect of time, after stimulation with Ovopel [(D-Ala6, Pro9-NEt)-mGnRH+metoclopramide] (1 granule∙kg-1 body weight) on semen quality indicators of common carp, Cyprinus carpio L., over the period of 72 h post injection. Materials and methods. The total volume of milt (TVM, mL), volume of milt per 1 kg of the male body weight (VOM, mL∙kg-1 b.w.), total sperm production (TSP, ×109), and their concentration (×109 mL-1) in milt were determined. Additionally, the motility of spermatozoa (%) by means of the subjective method and the osmotic pressure of seminal plasma (mOsm∙kg-1) were determined. The milt was collected 24 h (group I, n = 10), 48 h (group II, n = 10), and 72 h (group III, n = 10) after stimulation with Ovopel. Results. No significant differences (P > 0.05) in the main parameters of milt i.e., the motility and concentration of spermatozoa in the milt, as well as in the osmotic pressure of seminal plasma were found between the experimental groups of the fish. Higher TSP and VOM values were recorded 24 h after Ovopel injection compared to samples obtained after 72 h (P <0.01 and P < 0.05, respectively) and 48 h (P > 0.05). TVM values were also higher at 24 h after the injection than those noted at 48 and 72 h (P < 0.01). Conclusion. The lack of significant differences in the motility and concentration of spermatozoa in milt at 24, 48, and 72 h after injection indicate that time after Ovopel administration does not have an influence on the main indicators of common carp milt quality. However, we noted significant differences in TSP, TVM, and VOM between samples obtaining 24 h and 72 h after hormonal stimulation. The highest quantity indicators i.e., the number of spermatozoa in milt and volume of obtained milt noted for samples obtained after 24 h suggest that this time is better for milt sampling than time after 48 and 72 h.
Background. The abundance of club cells in epidermal tissue of fishes in the superorder Ostariophysi is a poorly understood phenomenon. Previous results have suggested that epidermal club cells have a generic role in response to injury and that they display intense phagocytotic activity, having an anti-parasitic function in the host. Earlier works suggested that club cells are usually located in the middle of the epidermis and that they do not communicate with the epidermal surface or do it only when the epidermis has been ruptured by predation. The presently reported study focused on the alterations of club cell activity in carp epidermis induced by ectoparasite, Ichthyophthirius multifiliis.We hoped that our observations would help to understand the function(s) of these cells. Materials and methods. This study was based on 200 four-month old common carp, Cyprinus carpio L., with mean body weight of 65 ± 5 g. The fish were experimentally infected with theronts of Ichthyophthirius multifiliis. In sequential days post infection, samples of fins and body skin were collected for histological and histochemical examination. The correlation between club cell densities and mucous cell densities was analysed using Pearson correlation analyses. Results. A local reduction of mucous cells occurred after theront invasion-induced proliferation, and increased club cell density around the parasite during the growth of trophonts. After parasites left the skin due to salt-water treatment, a decrease in the number of club cells was detected. During reinvasion the decrease in parasite activity in areas of club cells proliferation was not noted. It was found that giant mature club cells were opened on the surface. Conclusion. Club cells have no anti-parasitic function against I. multifiliis and these mature cells released their viscous secretion into water. The high density of club cells in the epidermis compensates an overall low density or absence of mucous cells. As it can be hardly concluded that the function of club cells is phagocytic removal of cell debris, an integrated research on mucosal immune mechanisms, as well as studies on epidermal tissue responses on product(s) released by club cells (“alarm substance cells”) should be carried out in the future.
Stone moroco [Pseudorasbora parva (SCHLEGEL, 1842)] represents a newly introduced fish species in the waters of the western Serbian districts. Some ecological features (age, growth rate, nutrition) were anałyzed on the sample comprising 96 individuals caught at the reservoir profiles.
The parameters of length–weight relations for Anatolian khramulya, Capoeta tinca (Heckel, 1843) from 10 inland bodies of water of the Samsun Province, Northern Turkey were presented. The b values of the length–weight relations ranged from 2.5444 to 3.3517. For nine freshwater resources in Samsun, this study is the first reference on length–weight relation of Capoeta tinca.
Background. Cobalt (Co) is an essential mineral required in trace quantity in the diet of fish. Although freshwater fish are capable of accumulating adequate quantity of trace minerals from the medium, Co is extremely scarce in the freshwater resources. Therefore, freshwater fish require a supplement of Co in the diet. Since information regarding requirement of Co for growth and its balance in the body of fish is poorly documented there is scope to quantify requirement of cobalt for common carp. Materials and Methods. Two separate trials were made with fingerlings of common carp, Cyprinus carpio: a growth trial made in outdoor cement tanks for 60 days and a digestibility trial made in 15-L glass aquaria in the laboratory for 7 days. Four experimental diets (average crude protein 30.94%) supplemented by four different levels of Co (0.00%, 0.05%, 0.10%, and 1.0%) were formulated and tested in these two trials. Growth was evaluated from gain in weight, specific growth rate, feed conversion ratio, and deposition of protein and lipid in the body of the fish. Apparent protein digestibility (APD) of the diets was evaluated from the proportion of chromium (Cr) and protein in the diet and faeces. Water qualities were checked every week and levels of Co in liver, kidney, gill, gut, and caudal trunk of the fish were determined by atomic absorption spectrophotometer at the end of 60 days in the growth trial. Results. Fingerlings of C. carpio fed 0.1% to 1.0% cobalt-supplemented diet (CSD) showed significantly higher growth than the control diet. Although conversion rate, weight gain and SGR were significantly higher in 0.05% CSD as compared with control diet (0.00% CSD), there was no significant difference in apparent protein digestibility (APD), apparent net protein utilization (ANPU), and deposition of crude protein in the body between fish fed control and 0.05% CSD. Fish fed 1.0% CSD showed significantly higher level of Co in different tissues as compared with other diet groups. Conclusion. It is concluded from the present study that dietary supplement of Co (0.1% to 1.0%) serves as a growth promoter for common carp. Increased growth with no additional tissue burden of Co is achieved at 0.1% dietary Co, while a higher level of supplement (1.0%) may result in increased deposition of Co in different tissues.
Background. The topmouth gudgeon, Pseudorasbora parva (Temminck et Schlegel, 1846) (known also as stone moroko), is an alien species of the fish fauna of many European freshwater ecosystems. In large quantities, its may affect negatively the fish fauna and the functioning of ecosystems. It competes for food with native fish species and they occupy their habitat. The knowledge about the invasion dynamics may help to find suitable control measures. The aim of this study was to find and observe the population of P. parva in a small river. Materials and methods. Inventory fishing on three sampling sites at the Ciemięga River was carried out from 2003 to 2007. The abundance and density of P. parva in the river were calculated. The size distribution of P. parva and its length–weight relation were determined. Abundance, density, length, and mass of the fish from all study sites were analyzed statistically. Results. P. parva was first recorded in the Ciemięga River in the autumn of 2005. The abundance of P. parva in relation to the structure of the local ichthyofauna was the smallest in the spring and the highest in autumn. The total length of P. parva ranged from 15 to 104 mm, and the mass from 0.2 to 10.4 g. Taking into account the number of the specimens, P. parva constituted between 0.9% and 57.2% of the local ichthyofauna, while its mass ranged from 0.0% to 35.1% depending on the study site and the season. The density of P. parva ranged from 72.9 to 6.5 CPUEn, while the mean value for the river was 30.6 CPUEn. Conclusion. In Polish waters P. parva is an alien species and there is still little information about its occurrence in the flowing waters. Since 2005, this species was present almost in all inventory catches in the Ciemięga River. Its presence was dependent on the kind of the habitat and the presence of predatory fish species (eg brown trout). P. parva occurring numerous may be a lot of competition for native fish species, and therefore an important problem is to understand his habitat preferences and interactions with native fish species.
Background. In Indian carp culture, a 1 : 1 mixture of rice bran and oil cake is traditionally used as feed in powder form; it is nutritionally inadequate and also a large part of it goes unutilized as the fish cannot feed on the powder effectively. With a view to improve its nutritional quality and reduce the wastage, the mixture was supplemented with soya and maize flours and converted into pellet form. The performance of the supplemented diets was evaluated by feeding them to catla, Catla catla, one of the popular Indian major carps. Materials and methods. Two test diets were formulated supplementing the feed mixture with soya flour (10% and 20%) and maize flour (39%), replacing equal quantities of rice bran and oilcake. These diets along with the control diet were fed to triplicate groups of catla fry maintained in outdoor cement tanks for 120 days. Results. The test diets enhanced growth and net fish production significantly (P < 0.05). This resulted in higher economic returns under the two test treatments. Survival of fish ranged from 74% to 76%. Carcass of fish receiving the test diets had significantly (P < 0.05) higher lipid and lower moisture levels. Digestive enzyme activity was affected positively (P < 0.05) by the test diets. Conclusion. Replacing rice bran and oilcake from the traditional feed mixture with soya and maize flours proved economically viable.
Background. Animals acquire new skills due to conditioned responses resulting from a sequence of neutral and unconditioned stimuli affecting their bodies; there is a constant time interval between the stimuli. Both stimuli become associated after a number of repetitions and animal bodies start to respond to the initially neutral stimulus as to the unconditioned one. The aim of this study was to determine whether exposure of fish to a pyrethroid—deltamethrin (an active ingredient in Decis 2.5 EC pesticide that disturbs the proper operation of the nervous system) affects the ability to learn in common carp. Materials and Methods. Production of new types of behaviour was triggered in fish by using the unconditioned stimulus (an irritating electric impulse) and the conditioned stimulus (light). The experiment was performed in a tank divided into two compartments where fish could swim freely from one compartment to another. The ability to learn was assessed on the basis of the following types of behaviour evoked in response to the conditioned stimulus: locomotor agitation, preference to occupy or avoid certain areas in the tank, an escape response, etc. Results. In fish exposed to 0.35 μg · L–1 deltamethrin (for common carp concentration 10 times lower than lethal) for 35 min the ability to produce and remember the conditioned defence response was reduced and time interval since presenting the conditioned stimulus till occurring the initial signs of the conditioned response was prolonged. Conclusion. The classical conditioning method revealed that sublethal concentration of deltamethrin restricted ability to learn and retain information in common carp, despite absence of observable intoxication symptoms.
Background. Common carp, Cyprinus carpio, is a model organism within Teleostei. Oligopeptides are a new and promising alternative source of amino acids in animal as well as in human nutrition. In common carp, the membrane protein that transports oligopeptides across the enterocyte membrane is encoded by the gene PepT1 (SLC15A1). The aim of this paper was to sequence the PepT1 (SLC15A1) in common carp. Materials and Methods. Intestine samples were isolated from six-week old common carp. Total RNA was isolated using a Trizol method. Reverse transcription was used to synthesize cDNA. Two different pairs of primers were designed, according to the zebrafish (Danio rerio) PepT1 sequence, and used for PCR. The amplified DNA was isolated by electrophoresis, cloned (pCRII-TOPO vectors), sequenced, and subjected to in silico analysis. Results. Two nucleotide fragments of the PepT1 gene were obtained and analyzed using bioinformatic tools. Both fragments showed a high degree of homology with the known PepT1 genes of other teleosts, mammals, and birds. High homology of the PepT1 gene, and similar primary protein structure among the aforementioned taxa probably reflects the conservative function of the PepT1 protein product. Both fragments of the PepT1 gene were deposited in GenBank (FJ556590; FJ529670). Conclusion. The sequenced fragments of the common carp PepT1 gene will allow evaluation of PepT1 expression in the intestines of fish fed diets containing various forms of protein, which is an issue of importance regarding fish nutrition and its import for aquaculture.
Background. The Ganga River (Ganges) is known of its rich fish diversity which is adversely affected by degrading environmental and ecological conditions. There are more than 300 exotic fish species in India. Many of them (particularly Cyprinus carpio) escaped from confinement and are now present in the Ganga River, challenging its ecological equilibrium. The aim of this project was to study the population characteristics of Cyprinus carpio (i.e., the abundance, size range, food and feeding, gonado-somatic index (GSI), gonad maturity stage, maturity and breeding) and the altered fishery dynamics. Materials and Methods. The commercially caught fishes, including the exotic ones, were identified, examined and measured. Important indices such as: the abundance index of exotic fishes, the gonado-somatic index (IG), as well as the gonad maturity stage of the escapee C. carpio were determined. The gut content was also analyzed to identify food items and thereafter the food richness, diet breadth, and gut repletion index (GRI%) were calculated using the Simpson’s diversity index. Results. C. carpio introduced about sixty years ago for aquaculture has now been found to invade into the Ganga, the largest river of the country contributing significantly to the fishery. The abundance index of C. carpio ranged from 12.2% to 45.5 % in 250 km long river stretch of the Ganga River flowing along Kanpur to Varanasi in the state of Uttar Pradesh. The gut content analysis revealed the presence of Eichhornia (9.8%), Pistia (11.6%), Nymphaea (8.5%), annelids worms (7.8%), algae (20.2%), mud and detritus (14.8%). The calculated diet breadth ranged from 0.82% to 0.91%; food richness ranged from 12 to 16, and the gut repletion index (GRI%) was 100% at different sampling sites. Mature females represented all the six reproductive stages with varying gonadosomatic index (IG) (0.5 to 30) in different catches. Presence of spent, ripe, and mature females was recorded. Conclusion. Abundance of exotic fish primarily C. carpio and Oreochromis niloticus in the fishery of the Ganga River was observed. The gonado-somatic index (IG) and the presence of all the six gonadal stages confirmed that C. carpio established its breeding population. The dominant catch of exotic fishes negatively impacted on the important indigenous fishes particularly Indian major carps (Catla catla, Labeo rohita, and Cirrhinus mrigala). The change in dynamics of the fishery due to the presence of common carp attracted attention to conserve rich indigenous germplasm facing threats of shifting from their natural habitats.
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