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Effect of different sucrose and nitrogen levels in the medium on chlorophyll and anthocyanin content in Clematis pitcheri shoots cultured in vitro at different temperatures

100%

Kawa-Miszczak L., Wegrzynowicz-Lesiak E., Gabryszewska E., Saniewski M.

Journal of Fruit and Ornamental Plant Research

2009

tom 17

nr 1

113-121

The aim of the work presented was to determine the chlorophyll and anthocyanin accumulation in Clematis pitcheri shoots cultured in vitro at different temperatures on the medium with various sucrose and nitrogen level. Two concentrations of sucrose: 10 g-l-1 and 30 gT1, and two levels of nitrogen compounds: 100% and 50% of standard MS strength, were used. Shoots were cultured at 15°C, 20°C and 25°C. It was found that plantlets of C. pitcheri grown at 20°C contained the highest and at 15°C the lowest content of chlorophyll. The sucrose concentration in the medium had no or only a slight effect. Lower level of nitrogen compounds (50% N) stimulated accumulation of chlorophyll in shoots as compared to a normal strength (100% N), with the highest differences at 15°C. High sucrose (30 g-l-1) and nitrogen (100% N) concentrations and low temperature (15°C) significantly promoted anthocyanins accumulation. Reduction of nitrogen compounds level in the medium to 50% and lowering sucrose concentration to 10 gT1 leads to decrease of anthocyanins accumulation in the shoots cultured at 15°C and 20°C. In the case of explants cultured at 25°C, the sucrose and nitrogen concentration in the medium had no or only a slight effect on accumulation of anthocyanins in the shoots.

Carbohydrate content in Clematis pitcheri shoots cultured in vitro depending on temperature and on the level of sucrose and nitrogen in the medium

80%

Kawa-Miszczak L., Gabryszewska E., Wegrzynowicz-Lesiak E., Saniewski M.

Zeszyty Problemowe Postępów Nauk Rolniczych

2008

tom 524

The aim of the present work was to determine the carbohydrate content in plantlets of Clematis pitcheri cultured in vitro depending on the temperature (15°C, 20°C and 25°C) and sucrose (10 and 30 g·dm⁻³) and nitrogen (100% and 50% of normal MS strength) level in the medium. 100% N meant 1.90 g KNO₃ and 1.65 g NH₄NO₃, and 50% N meant 0.950 g KNO₃ and 0.825 g NH₄NO₃ in 1 dm⁻³ of MS medium. The highest accumulation of starch in shoots took place at 30 g·dm⁻³ of sucrose and lower level of nitrogen compounds in the medium. Also, at 30 g·dm⁻³ of sucrose in the medium the content of fructose in shoots was higher and independent from the level of nitrogen. The temperature of 25°C stimulated glucose accumulation in shoots at the highest degree at 30 g·dm⁻³ of sucrose and lower level of nitrogen. Regardless of the treatment, no sucrose was found in plantlets. Generally, the higher concentration of sucrose in the medium the higher content of sum of carbohydrates in plantlets. A lower nitrogen compound level (50% N) led to increase of glucose and starch content in shoots, and had a smaller effect on the level of fructose. Shoots cultured at the temperature of 20°C and 25°C accumulated more carbohydrates than at 15°C.

Ograniczanie wyników

1 Journal of Fruit and Ornamental Plant Research

1 Zeszyty Problemowe Postępów Nauk Rolniczych

2 Gabryszewska E.

2 Kawa-Miszczak L.

2 Saniewski M.

2 Wegrzynowicz-Lesiak E.

1 2009

1 2008

Wyniki wyszukiwania

Effect of different sucrose and nitrogen levels in the medium on chlorophyll and anthocyanin content in Clematis pitcheri shoots cultured in vitro at different temperatures

Carbohydrate content in Clematis pitcheri shoots cultured in vitro depending on temperature and on the level of sucrose and nitrogen in the medium