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The nutritive value and terapeuthic activity of watermelon seeds is known, but up to day no studies on isolation and characterisation of their melanin were conducted. The aim of the study was to evaluate the antioxidant, antibacterial and light barrier properties of raw and purified melanins isolated from watermelon seeds. Native melanin was isolated from seeds by alkaline extraction. Obtained pigment was purified by acid hydrolysis. Chemical tests and FT-IR analysis were conducted to determine the melanin nature of the isolated pigments. UV-Vis, transmittance and colour properties were evaluated spectrophotometrically. Antioxidant activity was determined using ABTS and antibacterial activity through a well diffusion method. The results of the study demonstrated that melanins isolated from watermelon seeds had antioxidant, light barrier and antibacterial properties. A purified form of melanin had higher antioxidant activity and light barrier properties than the raw form. Both melanins inhibited the growth of Enterococcus faecalis and Pseudomonas aeruginosa. Watermelon seeds may be considered as a promising source of natural melanin which possess remarkable therapeutic action that can support the traditional use of this plant in the ethnomedicine.
Leaf beetles and fruitfly infestation greatly limits water melon (Citrullus lanatus) production throughout the tropics. The efficacy of aqueous plant extracts applied at 5 % w/v concentration of two botanicals (neems seed oil and garlic bulb) and 1.5 melt of one synthetic insecticide (karate 2.5 EC) against their infestation were evaluated. The effects of these treatments including control on the number of the insects, number of leaves, vines, length of vines, number of fruits, fruit weight, fruit damaged and undamaged were assessed during 2012 and 2013 cropping seasons in Lawanti, Gombe state, Northern Guinea Savannah region of Nigeria. Result of the study showed that neem seed oil and garlic bulb treatments at 5 % w/v were effective against the leaf beetles and water melon fruitfly as the synthetic insecticide karate 2.5 EC in managing the water melon insect pests. Karate 2.5 EC significantly reduced the infestation of the insect pests of water melon and increased number of leaves, vines, length of vines, number of fruits, fruit weight and number of undamaged fruits/plot followed closely by neem seed oil (A. indica) and garlic bulb (A. sativum) extracts. Water melon a growth and fruit yields were appreciably improved in these treatment while untreated control water melon crops had more leafs beetles and fruitfly infestations, poor plant growth and lower fruit yields. Both botanicals can thus serve as alternatives to synthetic insecticides for managing water melon leaf beetles and fruitfly attack.
A field experiment was conducted from August to November, during the 2012 and 2013 cropping seasons at the Research Farm, University of Agriculture, Makurdi, Nigeria, to identify the optimum plant density of okra and intercropping effects on yields of egusi melon-okra mixture and to assess the yield advantages of the intercropping system. The experiment was a 3x3 split plot arrangement of treatments, fitted in a randomized complete block design (RCBD), replicated four times. The intercropping (sole egusi melon, sole okra and egusi melon-okra mixture) constituted the main plots, while the population densities of okra (33,000, 40,000 and 50,000 plants ha-1 equivalent) into egusi melon were allocated to the subplots. Results of study showed that to maximize intercrop yield of okra in an egusi melon-okra intercrop, the optimal population density of okra is 33,000 plants ha-1, while that of 40,000 plants ha-1 is optimal to maximize intercrop yield of egusi melon. Intercropping egusi melon and okra significantly (P≤0.05) reduced yields of egusi melon (37.5 % and 40.5 % respectively, in years 2012 and 2013) and that of okra (9.7 % and 16.9 % respectively, in years 2012 and 2013). The highest mean land equivalent ratio value of 1.57 and highest land equivalent coefficient values of 0.60 and 0.63 respectively, in years 2012 and 2013, were recorded for okra sown into egusi melon at the population density of 40,000 plants ha-1. It is most advantageous having both crops in intercrop when okra is sown into egusi melon at the population density of 40,000 plants ha-1. This should therefore be recommended for Makurdi location, Nigeria.
A field experiment was conducted from August to November, during the 2012 and 2013 cropping seasons at the Research Farm, University of Agriculture, Makurdi, Nigeria, to evaluate the yield effects of egusi melon-okra intercrop as influenced by the interaction of time of introducing okra x cropping systems, and to assess the yield advantages of the intercropping system. The experiment was a 3x3 split plot arrangement of treatments, fitted in a randomized complete block design (RCBD), replicated four times. The cropping systems (sole egusi melon, sole okra and egusi melon-okra mixture) constituted the main plots, while the time of introducing okra (mid August, late August and early September) into egusi melon were allocated to the subplots. Results of study showed that to obtain optimal intercrop yield of okra in an egusi melon-okra intercrop, it is appropriate planting okra at the same time with egusi melon in mid August, while introducing okra into egusi melon in late August is appropriate to obtain optimal intercrop yield of egusi melon. Intercropping egusi melon and okra significantly (P≤0.05) reduced yields of egusi melon (37.5 % and 40.5 % respectively, in years 2012 and 2013) and that of okra (9.7 % and 16.9 % respectively, in years 2012 and 2013). The highest mean land equivalent ratio value of 1.57 and highest land equivalent coefficient values of 0.53 and 0.70 were respectively recorded, in years 2012 and 2013, for okra introduced into egusi melon in early September. These recommendations are appropriate for Makurdi location, Nigeria.
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