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Ultrasonic (high frequency/low intensity) stimulation of Chlorella vulgaris is shown to intensity population growth. The effeet is caused by a change in the cellular membrane potential as a resuh of increased permeability of the membrane; metabolie rate is thus determined by the cell′s energy demand. It is suggested tbat sonicatíon can be applied in biotech- nological proeesses involving Chlorella.
Mercury is known to be toxic to a number of phytoplankton even at very low concentrations. The metal inhibited survival, growth and biosynthesis of chlorophyll, carbohydrate and lipid of the green alga Chlorella vulgaris at its toxic concentrations (≥ 0.1 μM), but induced the biosynthesis of proline. The addition of amino acids (Alanine, Aspargine, Glutamate, Histidine and Cysteine) to the growth medium had a significant impact on bioassay results. The toxicity was expressed differently depending on concentration and type of amino acid added to cultures. LC50s ranged from 0.68 ± 0.004 to 0.97 ± 0.008 μM and the acute toxicity of Hg2+ in amino acid supplemented media followed the order C > C + ALA > C + GLU > C + ASP > C + HIS > C + CYS. The static dose of the metal to the alga was found to be 1 + 0.006 μM but its inhibitory effect on the alga was drastically reduced with the addition of amino acids. There were positive correlations between growth and metabolic activities of the alga and concentration of amino acid added to cultures at the presence of static concentration of Hg2+. The results suggested that amino acids have moderating effects on toxicity of Hg2+ because of their ability to regulate the concentration of free Hg2+ ions in growth medium.
Our objective was the assessment of algal medium harmfulness for in vitro fibroblasts. The algal medium was from Chlorella cultures (Beijerinck 1890) grown in the presence of benzene, which was added before the inoculation of culture. The medium contained, besides the benzene residues, its various metabolites formed during culture, among them phenol and catechol. Its addition to fibroblast cultures resulted in a decrease in their growth intensity and protein content in fibroblasts as well as an increase in DNA content and 14C-thymidine incorporation intensity to fibroblasts. The latter effect was probably connected with the inhibition of cell-division and DNA damage reparations. The obtained results indicate that Chlorella algae, besides other hydrobionts, take part in the forming of the benzene-like pollution toxicity in water habitat.
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