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Allozyme electrophoresis (horizontal starch gel and PAGE) and histochemical staining techniques were used to study the genetic composition of an endemic southern African domestic dogCanis familiaris Linnaeus, 1758, the Africanis breed. Genetic differentiation was analysed at 21 protein-coding loci. The results were compared to those for three other populations/breeds: representatives of established Western breeds, crossbred dogs of Western descent from rural areas in South Africa, and indigenous Saluki dogs from the Middle East. Nine polymorphic loci were found (Ak-1,-2, Ck, Per, Hb, Po-A-1 to-3 andPo-Tf). Two unique alleles at the Ck and Po-A-2 loci separated the Africanis breed from the other groups. There were also significant differences between Africanis and the other breeds in pair-wise comparisons of allelic frequencies at polymorphic loci. An assignment test, fixation index values, gene flow and genetic distance values indicated a closer genetic association between the Africanis and Saluki breeds than with dogs of Western origin. This finding supports archaeological evidence that the endemic Africanis breed was introduced from the Middle East into Africa thousands of years ago, and not through later western influences. The average heterozygosity ranged from 0.106–0.15, with least heterozygosity in the Africanis and most in the rural crossbred group. The percentage of polymorphic loci, the mean number of alleles per locus (biologically more significant than heterozygosity), and conformation of genotypes to Hardy-Weinberg proportions showed no evidence of recent loss of genetic diversity in Africanis. Genetic differentiation and support of archaeological evidence by genetics indicate that the endemic southern African domestic dog breed is unique.
The aim of this study was to assess the density and diet composition of free-ranging dogs in Poland. The study was conducted in a field and forest mosaic in the central part of the country in the years 2005–2011. The density of the free-ranging dogs was assessed during night counts along repeated transect routes. The number of dogs seen and the feasible observation area were recorded to calculate density index for each control. The day and night counts captured data on group composition and penetration range. Diets were studied through scat analyses. The dog density ranged from 2.2–3.1 ind. km–2 depending on the area. Most dogs were observed alone, and 40% formed groups of 2 to 5 dogs. Most groups were recorded close to buildings, but the group organisation changed (especially during the daytime) with increasing distance from the buildings: 35% of dogs in the village were in groups, but 55% of dogs formed groups at a distance of more than 100 m from the buildings. The night proportion of dogs in groups was approximately 50%, regardless of the distance. Their scats contained mostly cereal given by farmers. The prey remains were game species: roe deer (1.3% of occurrences in summer and 12% in winter), brown hare (3–4%), small mammals (5–9.5%) and birds (approx. 1.5%). The study demonstrated that the abundance of dogs in the rural areas of central Poland may play an important role in the ecosystem.
The study aimed to define the anatomical and immunohistochemical characteristics of the vomeronasal organ in six male dogs. The organ was a bilateral structure located in the foremost ventral part of the nasal cavity. It was tubular in shape, and 25.36 ± 1.89 mm in length. While the caudal end of the organ was blind, the cranial one consisted of a bilateral vomeronasal duct. At the histological examination, two types of epithelium were seen: sensory and non-sensory. The vomeronasal organ was largely surrounded by "J shaped" vomeronasal hyaline cartilage. The perichondrium of the vomeronasal hyaline cartilage consisted of chondroblasts. At the higher magnification, the strong immune positive reaction with anti-oestrogen antibody was observed only in the sensory epithelium of the organ.
The retinal fascin 2 gene (FSCN2) underwent a molecular analysis, a search for polymorphisms and an evaluation as a candidate gene for retinopathies in dogs. Specific fragments of the gene encompassing partial exon 1 and intron 1, and exons 2-5 with respective introns were sequenced and these data were deposited in the GenBank database. Three distinct polymorphic sites detectable with PCR-RFLP were found - AM050719: g.237G > A, AM050719: g.525A > G, and AM050720: g. 1071A > G. No positive associations between these polymorphisms and the PRA-clinical status were observed in the investigated population consisting of Poodles, American Cocker Spaniels, and English Cocker Spaniels. In spite of that, the FSCN2 gene remains an excellent candidate gene for retinopathies in dogs and the results can contribute to further research in this field.
Reproductive behaviour of free-ranging dogs Canis familiaris Linnaeus, 1758 was studied in a village in the state of West Bengal, India. Increased synchronized breeding was the most striking feature of this study. October (late monsoon) represented the peak period of mating for the feral dogs. Of all courting males, only 41% were observed to mount and copulate. On average, each male mounted 5.47 ± 2.49 (mean ± SD) times per hour. Of all mountings, only 10% were successful matings, ie copulatory ties. There was a negative correlation between the number of courting males and the number of successful copulations. The average duration of copulatory tie was 15.73 ± 7.75 min. Several factors interrupting the duration of copulatory ties were identified. December was the peak period of pup rearing. Mean litter size was 5.70 ± 2.03 with a male-biased sex ratio 1.41:1. Only a single annual breeding cycle recorded here differed from the previous studies on European and American dogs. Mothers spent most of the time with their pups at the dens during the early stage of rearing. The duration of time spent at dens by mothers was minimum when the pups were highly mobile at the age of 10 weeks. The lactating mothers were observed to be more aggressive immediately following litter production. Typically, an old adult male remained near the den as a 'guard'.
The present paper describes a synaptonemal complexes analysis carried out on oocytes collected from the ovaries of one-day-old puppies of domestic dog (Canis familiaris). Ovaries have been collected from 20 individuals altogether. Synaptonemal complexes were identified in oocytes originating from two puppies. It is concluded that the onset of canine female meiosis may occur earlier than previously reported.
Knowledge of morphometric characteristics of Heterodoxus spiniger was widened on the basis of material collected from dog (Canis familiaris) from India. Study, mostly by scanning electron microscope, concerned the males, females and nymphs of H.spiniger. The functional role of some elements, particularly observed surface ultrastructures, has been pointed out. The differences between postembrional stages were determined as well as literature data on H.spiniger were critically analysed.
Two species of hookworms from genus Uncinaria have been found so far in Poland. Uncinaria stenocephala infects mainly dog, wolf and red fox, whereas Uncinaria criniformis is a parasite of mustelids (but it was also reported from red fox). 19 male and 29 female hookworms from red foxes have been compared with 10 male and 12 female worms from dogs. Hookworms from dogs were generally smaller than these from foxes, but no other morphological differences could be found. These hookworms were qualified to species Uncinaria stenocephala on the ground of morphology of male. Genomic DNA samples have been isolated from these hookworms and segments of rDNA including part of small subunit of ribosomal RNA gene; internal transcribed spacer 1 (ITS1); 5.8 S ribosomal RNA; internal transcribed spacer 2 (ITS2) and part of large subunit of ribosomal RNA have been amplified and sequenced. Sequences from Uncinaria obtained both from foxes and dogs have shown very high similarity to the sequence of Uncinaria stenocephala, so all examined hookworms have been classified as belonging to this species.
A population of urban free-ranging dogs Canis familiaris Linnaeus, 1758 was studied in Katwa, West Bengal, India. The analysis of changes in the density of the dog population over a period of 4 years revealed a considerable stability of this population. Mean (±SD) seasonal population density was 185 ± 19 dogs/km , ranging from 156 to 214 dogs/km . A sex ratio of 1.37:1 in favour of male was recorded in this study. High mortality (67%) occurred under the age of 4 months, and 82% mortality occurred within the age of 1 year. Among the adults, 24% mortality under the age of 2.6 year was recorded. Only a single breeding cycle and synchronization of breeding was observed. Immigration was observed as a crucial factor affecting the stability of this population.
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