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1

Ano1 is a better marker than c-Kit for transcript analysis of single interstitial cells of Cajal in culture

100%
Loera-Valencia R., Wang X.-Y., Wright G. W. J., Barajas-Lopez C., Huizinga J. D.
Cellular and Molecular Biology Letters
|
2014
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tom 19
|
nr 4
The interstitial cells of Cajal (ICC) drive the slow wave-associated contractions in the small intestine. A commonly used marker for these cells is c-Kit, but another marker named Ano1 was recently described. This study uses single-cell RT-PCR, qPCR and immunohistochemistry to determine if Ano1 could be reliably used as a molecular marker for ICC in single-cell mRNA analysis. Here, we report on the relationship between the expression of c-Kit and Ano1 in single ICC in culture. We observed that Ano1 is expressed in more than 60% of the collected cells, whereas c-Kit is found only in 22% of the cells (n = 18). When we stained ICC primary cultures for c-KIT and ANO1 protein, we found complete co-localization in all the preparations. We propose that this difference is due to the regulation of c-Kit mRNA in culture. This regulation gives rise to low levels of its transcript, while Ano1 is expressed more prominently in culture on day 4. We also propose that Ano1 is more suitable for single-cell expression analysis as a marker for cell identity than c-Kit at the mRNA level. We hope this evidence will help to validate and increase the success of future studies characterizing single ICC expression patterns.
2

Ultrastructural characteristics of myenteric plexus in patients with colorectal cancer

100%
Zauszkiewicz-Pawlak A., Godlewski J., Kwiatkowski P., Kmiec Z.
Folia Histochemica et Cytobiologica
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2017
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tom 55
|
nr 1
3
Content available remote

Does different approach during pancreatoduodenectomy influence intestinal migrating myoelectrical complex recovery? Study in experimental pig model

100%
Borycka-Kiciak K., Kiciak A., Zabielski R., Romanowicz-Barcikowska K., Tarnowski W., Bielecki K.
Journal of Physiology and Pharmacology
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2013
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tom 64
|
nr 3
4

Gallbladder interstitial Cajal-like cells and gallbladder contractility in patients with cholelithiasis: a prospective study

100%
Ding R., Wei J., Xu J.
Folia Histochemica et Cytobiologica
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2019
|
tom 57
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nr 2
5

Evidence of interstitial Cajal-like cells in the human gallbladder

100%
Pasternak A., Gajda M., Gil K., Matyja A., Tomaszewski K. A., Walocha J. A., Kulig J., Thor P.
Folia Histochemica et Cytobiologica
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2012
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tom 50
|
nr 4
6
Content available remote

Loss of interstitial cells of Cajal after pulsating electromagnetic field (PEMF) in gastrointestinal tract of the rats

100%
Kaszuba-Zwoinska J., Gil K., Ziomber A., Zaraska W., Pawlicki R., Krolczyk G., Matyja A., Thor P. J.
Journal of Physiology and Pharmacology
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2005
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tom 56
|
nr 3
Exposure to the magnetic field has remarkably increased lately due to fast urbanization and widely available magnetic field in diagnosis and treatment. However, biological effects of the magnetic field are not well recognized. The myoelectric activity recorded from the gastrointestinal and urinary systems is generated by specialized electrically active cells called interstitial cells of Cajal (ICCs). Thus it seems rational that ICC have significant vulnerability to physical factors like an electromagnetic field. The aim of this study was to evaluate the influence of pulsating electromagnetic field (PEMF) (frequency 10 kHz, 30ms, 300 µT burst, with frequency 1Hz) on ICCs density in the rat gastrointestinal tract. Rats were divided into two groups (n=32). The first group was exposed to PEMF continuously for 1, 2, 3, and 4 weeks (n = 16), and the second group (n=16) served as a control. Tissue samples of the rat stomach, duodenum and proximal colon were fixed and paraffin embedded. The tangential sections of 5µm thickness were stained immunohistochemically with anti-c-Kit (sc-168) antibody and visualized finally by DAB as chromogen (brown end product). C-Kit positive branched ICC-like cells were detected under the light microscope, distinguished from the c-kit-negative non-branched smooth muscle cells and from the c-kit positive but non-branched mast cells and quantitatively analyzed by MultiScan computer program. Apoptosis detection was performed with rabbit anti-Bax polyclonal antibody (Calbiochem, Germany) and LSABTM 2 visualization system. The surface of c-Kit immunopositive cells decreased after exposure to PEMF in each part of the gastrointestinal tract. Reduced density of ICCs was related to exposure time. The most sensitive to PEMF were ICCs in the fundus of the stomach and in the duodenum, less sensitive were ICCs in the colon and pacemaker areas of the stomach. No marked changes in ICC density in the pyloric part of the stomach were observed. We demonstrate that the PEMF induced apoptosis dependent decrease in ICC expression.
7
Content available remote

The vagal afferents discharge and myoelectrical activity in the gastric hyperalgesia model in rats

80%
Krolczyk G., Gil K., Zurowski D., Jung A., Thor P. J.
Journal of Physiology and Pharmacology
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2008
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tom 59
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nr 4
707-716
A long term exposure of the gastric mucosa to inflammatory factors is suspected to alter the normal stomach motility. The consequence of it is an abnormal sensomotor response to food causing dyspeptic symptoms. Our study aimed to investigate the vagal afferents activity and the gastro-duodenal slow wave response to the mild gastric mucosa inflammation in rats. The gastric mucosal inflammation was induced by addition iodoacetamide to drinking water for 5 days. The gastro-duodenal slow wave, vagal nerve recordings and the gastric mucosa examination were performed on 6th day. The iodoacetamide irritated gastric mucosa presented the minimal inflammatory infiltration with mast cells. The vagal afferent activity was significantly increased after iodoacetamide treatment from 0.3 ± 0.1 to 1.9 ± 0.58 Hz, (p<0.05). The gastric slow wave accurate frequencies extracted from the fast Fourier transform spectra accelerated from 0.08 ± 0.01 to 0.1 ± 0.02 Hz (p<0.05). The duodenal frequencies remained unchanged (from 0.64 ± 0.02 to 0.59 ± 0.1 Hz). These results suggest that mild gastric mucosa irritation sensitizes vagal afferents and alters gastric but not duodenal pacemaker activity which may contribute to dyspeptic sensations.
8

Different distribution of c-kit positive interstitial cells of Cajal-like in children’s urinary bladders

80%
Piaseczna-Piotrowska A. M., Dzieniecka M., Kulig A., Danilewicz M., Chilarski A.
Folia Histochemica et Cytobiologica
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2011
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tom 49
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nr 3
9
Content available remote

Physiological and morphological effects of long-term vagal stimulation in diet induced obesity in rats

71%
Gil K., Bugajski A., Kurnik M., Zaraska W., Thor P.
Journal of Physiology and Pharmacology. Supplement
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2009
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tom 60
|
nr 3
61-66
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