Wyniki wyszukiwania

1

The detection of enteroviruses in sewage using Caco-2 cells

100%

Wieczorek M., Kuryk L., Witek A., Diuwe A., Litwinska B.

Polish Journal of Microbiology

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2013

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tom 62

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nr 1

The work presented here demonstrates the utility of Caco-2 cells to detect enteroviruses in sewage. Viruses were concentrated by beef extract elution and organic flocculation prior to analysis by cell culture assays and RT-PCR. Enteroviruses were detected in all sewage samples, but only one sample was positive solely in RT-PCR assay. We proved that Caco-2 cells were more effective than RD and L20B cells in enterovirus isolation, depending on procedures used in the inoculation process.

2

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

75%

Basson M. D., Zeng B., Wang S.

Journal of Physiology and Pharmacology

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2017

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tom 68

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nr 3

3

Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

75%

Markowicz C., Schmidt M. T.

Acta Scientiarum Polonorum. Technologia Alimentaria

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2015

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tom 14

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nr 3

Background. The ability of lactobacilli to adhere to the surface of the intestine is an important functional characteristic which can largely determine the effective colonization of the intestinal tract by probiotics. The following study compares the adhesion efficiency of the twenty strains of Lactobacillus genus belonging to Casei group to the Caco-2 cells and gastrointestinal mucus. Material and methods. Twenty isolates of lactobacilli belonging to Casei group were tested. The ability of bacterial cells to adhere to mucus was examined using adhesion assay to gastrointestinal mucus. Obtained results were compared with adhesion efficiency to Caco-2 cells. Phylogenetic relationship between isolates was analysed by rep-PCR. Results. The results showed large differences in adhesion efficiency between strains, as well as differences in the efficiency of adhesion to the intestinal epithelial cells and mucus. Group similarity highlighted by a rep- PCR technique does not correspond with groups of similarity in terms of the characteristics of the ability to adhere to mucus or the epithelial cells of intestinal tract. Conclusions. Strains having a high adhesion efficiency to enterocytes do not always show a high adhesion efficiency to the mucus. This may indicate the presence of different and multiple factors responsible for adhesion efficiency of Lactobacillus group Casei strains to epithelial cells and mucus.

4

Molecular characterization and intracellular regulation of the human serotonin transporter in Caco-2 cells

75%

Iceta R., Mesonero J. E., Aramayona J. J., Alcalde A. I.

Journal of Physiology and Pharmacology

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2006

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tom 57

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nr 1

The serotonin transporter (SERT) has shown itself to be an effective pharmacological target in the treatment of mood disorders and some kinds of gastrointestinal syndromes. Most of the molecular studies of SERT in humans have been carried out using heterologous models. In this work, we have investigated the human enterocyte-like Caco-2 cell line as a potential "in vitro" model to study the human SERT. The results show that these cells express a SERT mRNA identical to the human brain SERT, and a 70 kDa protein immunodetected using a specific antibody. The SERT activity levels in Caco-2 cells increased in correlation with the onset and maintenance of the morphological and functional differentiation of the cells. Caco-2 SERT was also shown to be a high affinity (Kt=0.216 µM) saturable, Na+-dependent transporter that was inhibited by fluoxetine (IC50=17.6 nM). In addition, SERT activity was inhibited by the intracellular modulators protein kinase C and cAMP, either after short or long-term treatment. In short, the expression and molecular characteristics of the human SERT in Caco-2 cells indicate that this cell line may be an ideal tool to study in vitro the physiology and pharmacology of human SERT.

5

The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material

63%

Wieczorek M., Ciacka A., Witek A., Litwinska B.

Polish Journal of Microbiology

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2014

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tom 63

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nr 1

The work presented here demonstrates the utility of Caco-2 cells in the isolation of enteroviruses (EVs) from environmental and clinical materials. Thirty-two samples of cerebrospinal fluid positive in Pan-entero RT-PCR were taken for EV strain isolation in cell culture. Out of the 32 samples analysed, 22 (68.75%) were positive for enteroviruses by isolation in Caco-2 cells, and 10 (31.25%) were positive by isolation in RD cells. High viral titre in clinical specimens resulted in rate increase for isolation in Caco-2 cells and RD cells (87.5% and 50%, respectively). Also, the probability of isolation of enteroviruses from sewage in Caco-2 cells was 20 times higher that in RD cells. We proved that Caco-2 cells were more effective than RD cells in enterovirus isolation, irrespective of the material used in the inoculation process.

6

Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2

63%

Antushevich H., Krawczynska B., Kapica M., Herman A. P., Zabielski R.

Folia Histochemica et Cytobiologica

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2014

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tom 52

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nr 1

7

Gold nanoparticles phytoreduced with Cornus mas extract mitigate some of gliadin effects on Caco-2 cells

51%

Domsa E. M., Filip G. A., Olteanu D., Baldea I., Glichici S., Muresan A., David L., Moldovan B., Para I., Suciu M., Achim M., Negrean V., Andreica V.

Journal of Physiology and Pharmacology

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2020

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tom 71

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nr 2

8

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Digestion and absorption of phenolic compounds assessed by in vitro simulation methods. A review

51%

Tarko T., Duda-Chodak A., Zajac N.

Roczniki Państwowego Zakładu Higieny

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2013

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tom 64

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nr 2

Phenolic compounds are a group of key plant metabolites found abundantly in fruit and vegetables. Because of their antioxidant properties, they play a significant role in preventing various degenerative illnesses, tumours or cardiovascular disease. In nature, they are present in foods mainly as esters, glycosides and polymers which need to undergo enzymatic hydrolysis in the digestive tract or by the gut microflora before becoming absorbed. The biological properties of these phenolic compounds undergoing this degradation, are thus governed by their absorption as well as metabolism. Many methods are used to assess the rates and the degrees to which these substances are digested and absorbed, both in vivo and in vitro ones, where the former are the most reliable, although they suffer from various limitations. For this reason, many in vitro models have now arisen to simulate the function of human digestion in the attempt to faithfully re-create real-life conditions. Mechanisms of polyphenols absorption have been principally studied by intestinal epithelial cell models, in particular, those using the Caco-2 cell line.

9

Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment

51%

Dzierzewicz Z., Orchel A., Weglarz L., Latocha M., Wilczok T.

Acta Biochimica Polonica

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2002

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tom 49

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nr 1

Gut-derived adenocarcinoma Caco-2 cells were treated with sodium butyrate (NaB) at physiologically relevant concentrations. We characterized its effects on proliferation, differentiation, apoptosis, adhesion to the solid support and interleukin-8 secretion. Differentiation was determined by brush border alkaline phosphatase activity. Apoptosis was assessed by acridine orange and Hoechst stains. Differentiation and apoptosis were analyzed in both adherent and floating cell populations. The transformed Caco-2 cells did not retain their malignant phenotype in the presence of NaB. They appeared to undergo a change in the phenotype induced by NaB, as indicated by reduced proliferation, enhanced differentiation, stimulation of apoptosis leading to decreased viability of cells, and stimulation of interleukin-8 secretion. Considering all the above facts and data, we postulate that Caco-2 cells cultured in NaB supplemented medium could regain the phenotypic characteristics of the phenotype of the parent cell from which originated the Caco-2 line.

Ograniczanie wyników

The detection of enteroviruses in sewage using Caco-2 cells

The C-terminal region of the focal adhesion kinase F1 domain binds akt1 and inhibits pressure-induced cell adhesion

Lactobacillus strains belonging to Casei group display various adherence to enterocytes and mucus

Molecular characterization and intracellular regulation of the human serotonin transporter in Caco-2 cells

The utility of Caco-2 cells in isolation of enteroviruses from environmental and clinical material

Effect of apelin on mitosis, apoptosis and DNA repair enzyme OGG 1/2 expression in intestinal cell lines IEC-6 and Caco-2

Gold nanoparticles phytoreduced with Cornus mas extract mitigate some of gliadin effects on Caco-2 cells

Digestion and absorption of phenolic compounds assessed by in vitro simulation methods. A review

Changes in the cellular behaviour of human colonic cell line Caco-2 in response to butyrate treatment