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Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials

100%

Rajasekharan S. K., Ramesh S.

Polish Journal of Microbiology

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2013

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tom 62

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nr 3

Burkholderia cepacia is an opportunistic pathogen causing infections in patients with cystic fibrosis. Patients with implanted devices are prone to B. cepacia infections due to its ability to grow as biofilms. Knowing the importance of polysaccharides in a biofilm, enzymes that degrade them were targeted as a possible candidate for antibiofilm agents. In this study, the antibiofilm potential of cellulase against B. cepacia biofilms formed on various prosthetic materials was tested. Cellulase exhibited significant antibiofilm activity against B. cepacia without having much action on its growth, thus ruling out the chance of selection pressure and subsequent development resistance.

2

Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

84%

Navratilova L., Chroma M., Hanulik V., Raclavsky V.

Polish Journal of Microbiology

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2013

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tom 62

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nr 4

The strains belonging to Burkholderia cepacia complex are important opportunistic pathogens in immunocompromised patients and cause serious diseases. It is possible to obtain isolates from soil, water, plants and human samples. Taxonomy of this group is difficult. Burkholderia cepacia complex consists of seventeen genomic species and the genetic scheme is based on recA gene. Commonly, first five genomovars occurre in humans, mostly genomovars II and III, subdivision IIIA. Within this study we tested identification of first five genomovars by PCR with following melting analysis and RFLP. The experiments were targeted on eubacterial 16S rDNA and specific gene recA, which allowed identification of all five genomovars. RecA gene appeared as more suitable than 16S rDNA, which enabled direct identification of only genomovars II and V; genomovars I, III and IV were similar within 16S rDNA sequence.

3

Badania nad ograniczeniem populacji w glebie ważnego patogena cebuli - bakterii Burkholderia cepacia

84%

Kowalska B., Smolinska U.

Polish Journal of Agronomy

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2013

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nr 15

Celem badań było określenie, czy dodatek do gleby zakażonej bakterią Burkholderia cepacia makuchu rzepakowego, zmielonych nasion gorczycy sarepskiej lub antagonistycznego grzyba Trichoderma harzianum wpłynie na ograniczenie liczebności tego patogena. W glebie umieszczonej w kontenerach uprawiano cebulę odmiany Grabowska (Allium cepa L.). W trakcie uprawy analizowano populacje patogenicznej bakterii B. cepacia i innych mikroorganizmów glebowych oraz oceniano plon i zdrowotność cebuli. Doświadczenia prowadzono przez trzy sezony wegetacyjne. Stwierdzono, że liczba B. cepacia w glebie zmniejszała się wraz z upływem czasu we wszystkich kombinacjach. Istotny spadek liczebności B. cepacia zaobserwowano w kombinacjach z makuchem i gorczycą, szczególnie po upływie 3 i 9 miesięcy od zaaplikowania materiałów roślinnych. W tych kombinacjach stwierdzono także istotny wzrost ogólnej liczby bakterii, bakterii przetrwalnikujących i z grupy Pseudomonas, promieniowców oraz grzybów. Istotne różnice zaobserwowano zwłaszcza po upływie 1 miesiąca od dodania materiałów roślinnych. Wzrost plonu cebuli zaobserwowano po dodaniu makuchu rzepakowego zarówno w kombinacji zakażonej B. cepacia, jak i w kombinacji bez patogena. Dodatek zmielonych nasion gorczycy sarepskiej do gleby niezakażonej pozytywnie wpływał na plon cebuli, jednakże dodatek do gleby zakażonej znacznie obniżał plon.

4

Susceptibility of onion cultivars to infection by pathogenic bacteria

83%

Kowalska B., Smolinska U.

Progress in Plant Protection

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2014

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tom 54

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nr 2

There is limited information about the resistance of onion cultivars to bacterial diseases registered in Poland. Therefore, in the presented work 33 varieties of onion were examined for their susceptibility to infection by Burkholderia cepacia, B. gladioli pv. Alliicola and Pectobacterium carotovorum subsp. carotovorum. It was found that most of the studied cultivars were sensitive to infection by B. cepacia. In the case of P. carotovorum subsp. carotovorum and B. gladioli pv. alliicola the susceptibility of studied cultivars varied. Alonso F1 cultivar showed the highest resistance to bacterial infection.

5

Bakterie patogenne dla cebuli (Allium cepa L.)

67%

Kowalska B., Smolinska U.

Postępy Mikrobiologii

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2014

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tom 53

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nr 1

6

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Znaczenie bakterii Burkholderia cepacia dla rolnictwa i ochrony srodowiska naturalnego

67%

Kowalska B., Szczech M.

Postępy Nauk Rolniczych

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2005

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tom 52

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nr 6

25-37

Burkholderia cepacia complex (Bcc) was originally recognized as a pathogen of onions. It causes sour skin of onions, what is associated with the yellow/brown coloured soft rot of onion tissue. It is a disease very important economically. Additionally, Burkholderia cepacia has positive environmental effects. The bacteria is able to produce antimicrobial compounds that protect plants, as well as some factors that promote plant growth. B. cepacia also can degrade a wide variety of compounds including pollutants, and therefore it might be used as a bioremediation agent.

7

Choroby bakteryjne cebuli

67%

Kowalska B.

Owoce, Warzywa, Kwiaty

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2007

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nr 07

8

Prostaglandin-endoperoxide synthase genes COX1 and COX2 - novel modifiers of disease severity in cystic fibrosis patients

67%

Czerska K., Sobczynska-Tomaszewska A., Sands D., Nowakowska A., Bak D., Wertheim K., Poznanski J., Zielenski J., Norek A., Bal J.

Journal of Applied Genetics

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2010

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tom 51

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nr 3

Cystic fibrosis (CF) is one of the most common autosomal recessive diseases among Caucasians caused by a mutation in the CFTR gene. However, the clinical outcome of CF pulmonary disease varies remarkably even in patients with the same CFTR genotype. This has led to a search for genetic modifiers located outside the CFTR gene. The aim of this study was to evaluate the effect of functional variants in prostaglandin-endoperoxide synthase genes (COX1 and COX2) on the severity of lung disease in CF patients. To the best of our knowledge, it is the first time when analysis of COX1 and COX2 as potential CF modifiers is provided. The study included 94 CF patients homozygous for F508del mutation of CFTR. To compare their' clinical condition, several parameters were recorded, e.g. a unique clinical score: disease severity status (DSS). To analyse the effect of non-CF7X genetic polymorphisms on the clinical course of CF patients, the whole coding region of COX 1 and selected COX2 polymorphisms were analysed. Statistical analysis of genotype-phenotvpe associations revealed a relationship between the heterozygosity status of identified polymorphisms and better lung function. These results mainly concern COX2 polymorphisms: -765G>C and 8473T>C. The COX1 and COX2 polymorphisms reducing COX protein levels had a positive effect on all analysed clinical parameters. This suggests an important role of these genes as protective modifiers of pulmonary disease in CF patients, due to inhibition of arachidonic acid conversion into prostaglandins, which probably reduces the inflammatory process.

9

Bakteryjne choroby cebuli

67%

Kowalska B.

Nowości Warzywnicze

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2006

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tom 43

67-74

10

Na polach cebuli w 2004 roku

59%

Robak J.

Hasło Ogrodnicze

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2005

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nr 03

Ograniczanie wyników

Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials

Possibilities in identification of genomic species of Burkholderia cepacia complex by PCR and RFLP

Badania nad ograniczeniem populacji w glebie ważnego patogena cebuli - bakterii Burkholderia cepacia

Susceptibility of onion cultivars to infection by pathogenic bacteria

Bakterie patogenne dla cebuli (Allium cepa L.)

Znaczenie bakterii Burkholderia cepacia dla rolnictwa i ochrony srodowiska naturalnego

Choroby bakteryjne cebuli

Prostaglandin-endoperoxide synthase genes COX1 and COX2 - novel modifiers of disease severity in cystic fibrosis patients

Bakteryjne choroby cebuli

Na polach cebuli w 2004 roku