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Fat-inducing transcript (FIT) are endoplasmic reticulum-resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of storing fat. In this study, applying the PCR-SSCP and DNA sequencing methods, polymorphism of the FIT gene were detected. A total of 708 individuals from four Chinese cattle breeds were examined. The results showed that only P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.199G > T resulting In L124L) and a missense mutation (NM_001103095: m.434G > T resulting in V176L). The associations between polymorphic loci and selected growth traits of indigenous Nangyang cattle were analysed,and significant associations were found in body weight at the age of 12 months and mean daily live weight gain. The body weight at month 12 of life and mean daily live weight gain of individuals with genotype AA were by 3.75% and 4.88% higher than of those with genotype AB, respectively. Hence, it was suggested for the first time, that genotype AA could be regarded as molecular marker for superior body weight and daily live weight gain in Chinese Nanyang cattle.
Physical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in species whose assembled genome sequences are available. However, in this work we evidence the existence of errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results indicate that FISH mapping in cattle is still useful.
Ultrastructural study of spermiogenesis and of the spermatozoon of Carmyerius endopapillatus has enabled to describe some characteristics of this digenea. The intercentriolar body situated between the two striated roots and the two centrioles, presents a symmetric organization. Both external bands of this intercentriolar body are made up of a row of granules. During spermiogenesis, a flagellar rotation of 90° is described. The old spermatid does not present external ornamentations. The spermatozoon is characterized, in its anterior region, by the presence of a lateral expansion exhibiting one spinelike body. In C. endopapillatus, external ornamentations are localized only at the level where the lateral expansion appears. The posterior extremity of spermatozoon exhibits a nucleus surrounded by a plasmic membrane lacking microtubules, but presenting a small lateral expansion. This is the first species of Gastrothylacidae family studied by transmission electron microscopy.
Nineteen ВАС clones were identified by hybridization of the bovine genomic ВАС library CHORI-240 with mixed CSN1S1- and CSN3-specific probes. Two of the clones were shown to contain the genes CSN1S1, CSN1S2, CSN2, STATH and CSN3, and five were proved to include the genes CSN2, STATH, CSN1S2 and CSN3. These data showed that the ВАС contig was established for the whole casein cluster, including all known five genes.
The analysis of fossil and sub-fossil bones of wild Bos primigenius Bojanus, 1827 and domestic cattle Bos taurus Linnaeus, 1758 has been handicapped by the absence of modern comparative material. The feral cattle of Amsterdam Island have lived in the wild since 1871 and have been free from artificial selection since that period. We give here a complete description of metapodial bones of this population in order to offer archaeologists a modern comparative material with patterns of sexual dimor­phism and extent of intra- and inter-individual variability. This work is based on the measurements of 90 sets of 4 metapodials belonging to 48 adult females and 42 adult males. We show that the cattle of Amsterdam Island are morphologically homogeneous, thus probably forming a single breed. Sexual dimorphism is important and was studied by univariate comparisons and ordination techniques. A discriminant analysis revealed that differences in depth of diaphysis alone could correctly classify 96.7% of individuals as far as metacarpal bones are concerned, whereas differences in breath of distal end alone could correctly classify 91.1% of individuals when metatarsal bones were meas­ured. Inclusion of two more variables increased the accuracy to 98.8% and 97.8% of individuals correctly classified for metacarpal and metatarsal bones, respectively. Allometric relations within sexes are described and should prove to be usefull to archaelogists who work with fragmentary material and wish to estimate lacking measurements. Comparisons of size and shape of metapodial bones with data from the literature reveal that the feral cattle of Amsterdam Island are smaller than aurochs and recent breeds of domestic cattle, but that they compare well with old breeds of domestic cattle and also recent breeds of Bos indicus living in Africa.
Here we present the first data on chromosome banding for Capra falconeri heptneri (Zalkin, 1945) (Bovidae: Caprinae), a critically endangered subspecies of the markhor, and compare its G- and C-banding patterns with those of the congeneric Alpine ibex C. ibex Linnaeus, 1758 and the evolutionary more distant cattle Bos taurus Linnaeus, 1758. The two goat species have identical karyotypes whereas B. taurus, which has the same diploid number (2n = 60) and autosomal fundamental number (aFN) differs in the morphology of two pairs of autosomes (9 and 14) and of the X chromosome, as well as in the amount of C heterochromatin. Although the study supports the earlier idea of karyotype homogeneity within the genus Capra, new comparative cytogenetic data for unstudied yet congeneric and other related species are necessary for our understanding of the pattern of chromosome evolution within the subfamily Caprinae and, more broadly, the family Bovidae.
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