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Tropical theileriosis is considered one of the most economically important tick borne diseases that cause significant mortality and morbidity to livestock. In the context of epidemiological studies on livestock in Egypt, this report investigated the spread of Theileria annulata among diseased farm cows (Bos indicus) over one year. Blood samples collected from 130 cows were investigated by routine staining and 64 samples were investigated by PCR assay using two different probes targeting tams-1 gene. Microscopy revealed the infection of 33.8% of animals with Theileria while PCR detected infection in 51% of animals with one primer pair and the other primer pair detected the infection in 31% of animals. Combined PCR results indicated the infection of 68.8% of animals with T. annulata. Seasonal fluctuation of parasite infection was evident with the highest infection percentage and prevalence recorded during summer based on both microscopy and PCR data. For the first time, the current study reports the presence of two T. annulata isolates based on tams-1 gene partial sequence in Egypt. Targeting polymorphic genes for parasite detection may result in underestimation of infection and target gene diversity has to be considered. The high infection with these pathogens in the clinically ill cows necessitates implementing serious programs to minimize their economic burden on the Egyptian farming industry.
The analysis of fossil and sub-fossil bones of wild Bos primigenius Bojanus, 1827 and domestic cattle Bos taurus Linnaeus, 1758 has been handicapped by the absence of modern comparative material. The feral cattle of Amsterdam Island have lived in the wild since 1871 and have been free from artificial selection since that period. We give here a complete description of metapodial bones of this population in order to offer archaeologists a modern comparative material with patterns of sexual dimor­phism and extent of intra- and inter-individual variability. This work is based on the measurements of 90 sets of 4 metapodials belonging to 48 adult females and 42 adult males. We show that the cattle of Amsterdam Island are morphologically homogeneous, thus probably forming a single breed. Sexual dimorphism is important and was studied by univariate comparisons and ordination techniques. A discriminant analysis revealed that differences in depth of diaphysis alone could correctly classify 96.7% of individuals as far as metacarpal bones are concerned, whereas differences in breath of distal end alone could correctly classify 91.1% of individuals when metatarsal bones were meas­ured. Inclusion of two more variables increased the accuracy to 98.8% and 97.8% of individuals correctly classified for metacarpal and metatarsal bones, respectively. Allometric relations within sexes are described and should prove to be usefull to archaelogists who work with fragmentary material and wish to estimate lacking measurements. Comparisons of size and shape of metapodial bones with data from the literature reveal that the feral cattle of Amsterdam Island are smaller than aurochs and recent breeds of domestic cattle, but that they compare well with old breeds of domestic cattle and also recent breeds of Bos indicus living in Africa.
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