Wyniki wyszukiwania

1

A survey on the adjuvant role of naloxone alone or combined with alum in vaccination against fasciolosis in BALB/c mice

100%

Azizi H., Mirzaeei H., Bagheri A., Bazi A., Khamesipour A., Yaghoobi H., Mirzapour A., Khatami M., Elikaee S.

Acta Parasitologica

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2019

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tom 64

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nr 2

2

Peripheral blood and lymphatic organs in BALB-c mice during the progressive and regressive phases of Moloney sarcoma development

80%

Dzwonkowska B., Wlodarski K. H.

Folia Biologica

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2010

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tom 58

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nr 1-2

9-13

3

Immunomodulation of ALT-2 and TLR may collude in antigen specific T cell hyporesponsiveness: Proposed mechanism for elevated IL-10 levels in Balb/C mice

80%

Aparnaa R., Kaliraj P.

Acta Parasitologica

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2014

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tom 59

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nr 1

ALT-2, a novel antigen belonging to the chromadorea ALT-2 family of the filarial nematode is proved to clear filarial parasites in Jirds. In order to increase the protection efficacy by stimulating the cell mediated immunity, MPLA a detoxified derivative of LPS known to induce the cellular response, was used in this study as an adjuvant on mice models. ALT-2+MPLA formulation elicited a high titer of total IgG antibody, with profoundly increased levels of IgG2b. Reduced splenocyte proliferation was observed in immunized group when compared to control groups which could be attributed to many in vivo factors. The levels of IFN-γ were high in unstimulated MPLA group compared to ALT-2 stimulated MPLA group, suggesting that the ALT-2 antigen suppressed the IFN-γ levels. A high level of IL-10 was induced by the ALT-2+MPLA formulation, which inhibited the production of Th2 cytokines (IL-4, IL-5) and also reduced the Th1 cytokine (IFN-γ, IL-2) levels which are not in vogue with the classical MPLA adjuvant formulation. We propose a mechanism for this immunomodulation which involves a diminished expression of TLR-4, by which the filarial parasites have evolved to evade host immune mechanism.

4

Monitoring of BALB/C strain mice health, investigation of behavior, hematological parameters under the effect of an electromagnetic field

80%

Zymantiene J., Zelvyte R., Juozaitiene V., Oberauskas V., Noreika A., Juodziukyniene N., Aniuliene A.

Medycyna Weterynaryjna

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2019

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tom 75

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nr 03

The aim of this study was to investigate the effect of electromagnetic fields on BALB/c strain mice on their health, body weight, behavior characteristics, hematological parameters and histopathological findings in the brain. The mice of the experimental groups were exposed to electromagnetic waves by using Nokia 230 and Samsung 19300 Galaxy S III mobile phones situated at 2 cm from the cages. In the present study, it can be concluded that the exposure of mice to mobile phone radiation had an effect on the structure of the brain, behavior and body weight. The waves of mobile phones increased activity characteristics and changed some behavioral categories of mice and also decreased their body weight. Histopathological examination revealed mild edema of neutrophils and degeneration of some neurons and glial cells in the brains of experimental mice. The results of the present study showed that a using mobile phone had an influence on in vivo systems.

5

Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

80%

Lopez-Perez I. C., Collantes-Fernandez E., Rojo-Montejo S., Navarro-Lozano V., Risco-Castillo V., Perez-Perez V., Pereira-Bueno J., Ortega-Mora L. M.

Acta Parasitologica

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2011

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tom 56

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nr 3

Neospora caninum is transmitted from a cow to its foetus by vertical transmission and the timing of infection in gestation is an important factor in determining the disease outcome. Few studies have explored the role of the placenta in the outcome of N. caninum infection during pregnancy. Here, we described the N. caninum presence, parasite load, local immune response, and histopathological lesions at the materno-foetal interface after infection of BALB/c mice at early and late stages of gestation. In mice infected at early gestation, N. caninum DNA was detected in foetoplacentary units 7 days post-infection (PI) and in the placenta, but not in viable foetuses on day 14 PI, indicating that the parasite was multiplying primarily in the placental tissues without reaching the foetus. Moreover, parasite DNA was detected in resorptions, suggesting that foetal death could be a consequence of infection. An increase in IFN-γ, TNF-α and IL-10 expression was observed in N. caninum PCR-positive placentas, which could favour N. caninum foetal transmission and be harmful to both the placenta and the foetus. Histopathological analysis revealed necrosis affecting both the maternal and foetal sides of the placenta. At late gestation, transmission occurred rapidly following infection (day 3 PI), but parasite were rarely found. In addition, an increase in cytokine expression was observed in spleen and placental tissues from infected animals, while a downregulation in IL-4 expression was only observed in the spleen. Finally, necrosis in the placenta was limited to the maternal side, suggesting that the parasite is mainly multiplying in the placental tissue at this stage. Thus, the results of the present study indicate that the placenta may be actively involved in N. caninum pathogenesis.

6

Influence of caffeine on follicular development in Balb/c mice

80%

Balcerzak A., Tosik D., Bartel H.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2008

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tom 50

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nr 1

7

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Long term monitoring of Babesia microti infection in BALB-c mice using nested PCR

80%

Welc-Faleciak R., Bajer A., Bednarska M., Paziewska A., Sinski E.

Annals of Agricultural and Environmental Medicine

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2007

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tom 14

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nr 2

In this study we report the usefulness of nested PCR for screening of the persistent B. microti infections in rodent hosts. Female BALB/c mice were inoculated with 100 μl of donor blood infected with B. microti. Infections were detected using microscopic examination of Giemsa-stained blood smears. To determine whether B. microti DNA was present in blood and/or spleen tissue, nested PCR was performed targeting a specifi c fragment of the gene encoding the 18S rRNA. Blood was sampled every 10 days post-infection (dpi) until day 30, after which mice were sampled every 30 days until the end of experiment at 360 dpi. The most extensive parasitaemia (39% of infected erythrocytes) was observed at 10 dpi. Between 20-60 dpi, less then 1% of infected erythrocytes were detected in blood smears, and from 90 dpi onwards, infected erythrocytes were no longer observed. B. microti DNA was successfully amplifi ed from the blood of mice from 10 dpi until 180 dpi, as well as from spleens of infected mice at 10 and 20 dpi. The presented results show that nested PCR is the method of choice for monitoring infections of B. microti in the blood of rodent hosts, and could therefore be a tool for environmental monitoring of naturally infected rodents which are the predominant source of infection for tick vectors.

Ograniczanie wyników

A survey on the adjuvant role of naloxone alone or combined with alum in vaccination against fasciolosis in BALB/c mice

Peripheral blood and lymphatic organs in BALB-c mice during the progressive and regressive phases of Moloney sarcoma development

Immunomodulation of ALT-2 and TLR may collude in antigen specific T cell hyporesponsiveness: Proposed mechanism for elevated IL-10 levels in Balb/C mice

Monitoring of BALB/C strain mice health, investigation of behavior, hematological parameters under the effect of an electromagnetic field

Pathological and immunological findings in placentas from pregnant BALB/c mice infected with Neospora caninum at early and late stages of gestation

Influence of caffeine on follicular development in Balb/c mice

Long term monitoring of Babesia microti infection in BALB-c mice using nested PCR