Ograniczanie wyników

Czasopisma help
Autorzy help
Lata help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 110

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 6 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

Wyszukiwano:
w słowach kluczowych:  Aspergillus
help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 6 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
We have cloned and sequenced the two intervening transcribed spacers in the rDNA repeat unit of three Aspergillus species — A. nidulans, A. aw amort and A. wentii. The A. wentii and A. awamori spacers are almost identical and share a high degree of homology with the A. nidulans spacers. All spacers have a high G-C content (66%-76%) and the potential of forming complex secondary structures, which may indicate that they play a role in the maturation of pre-rRNA molecules.
A shift towards organic farming suggests amalgamation of organic resources against soil borne plant pathogens. The influence of metabolites of most ubiquitous Aspergillus spp., organic amendment extracts and their combined effect with Trichoderma virens were evaluated in vitro against Rhizoctonia solani. The minimum (36.1 mm) growth was attained by R. solani in co-culture with A. niger. The maximum (42.3 mm) inhibition of mycelial growth of the test organism was observed with culture filtrate of A. ochraceous followed by A. niger, A. fumigatus, A. flavus and A. terreus. Among organic amendment extractants, castor cake exhibited an additive effect on the growth of T. virens, however, the maximum (41.8 mm) suppressive effect on R. solani was observed with vermicompost. With the advance in time, the effect of organic amendment extracts increased markedly. Inhibition potential of culture filtrate mixturte of A. niger + T. virens and A. ochraceous + T. virens against R. solani was significantly higher in comparison to the other combinations.
Do niebezpiecznych substancji pochodzenia biologicznego spotykanych w żywności należą mikotoksyny. Są one produktami wtórnego metabolizmu grzybów strzępkowych. Charakteryzują się wysoką szkodliwością dla ludzi, zwierząt, jak również roślin wyższych. Mogą one być przyczyną ostrych zatruć, wykazują także silne właściwości mutagenne, kancerogenne, teratogenne i estrogenne. Spośród znanych obecnie grzybów strzępkowych, większość szczepów toksynotwórczych należy do trzech rodzajów: Aspergillus, Pénicillium i Fusarium. Mikotoksyny wyizolowano ze środków spożywczych głównie pochodzenia roślinnego, tj. zbóż i ich przetworów, jak również pochodzenia zwierzęcego, w tym mięsa i mleka.
Fifty four mould strains (32 Aspergillus and 22 Penicillium) isolated from clinical materials were tested using dilution method. Two test media were used: Yeast Nitrogen Base (YNB) and Czapek Dox (CD). The following drugs were tested: amorolfine, cyclopirox, itraconazole, and terbinafine. In the dilution method, the drugs were tested at four contrentrations: 0.1 mg/l, 1 mg/l, 10 mg/l, and 100 mg/l. No matter which medium and/or drug was used, terbinafine turned out to be most effective. The drug at a conceatration of 1 mg/l inhibited 88.9% of the strains in the CD medium. The MIC values in the YNB medium varied from 0,1 mg/l through 100 mg/1, however, 50% of the strains were inhibited at 1 mg/l or less. Itraconazole revealed a fairly good in vitro antifungal action, particularly in the CD medium: 77.8% of the strains were inhibited at 10 mg/l or less. Aspergillus fumigatus and Aspergillus flavus were most susceptible to itraconazole. The MIC values of cyclopirox amounted to 100 mg/l for all of the strains in the CD medium but not in the YNB where they varied from 1 mg/l to 100 mg/l. Amorolfine was the least effective drug. Most of the strains were inhibited at a concentration of at least 100 mg/l. The findings give evidence that the susceptibility of Aspergillus and Penicillium to the particular drugs is different, and that the results are influenced by the test medium.
The aim of this study was to test the air in a single hospital department for fungal contamination. The department included three fully protected rooms with laminar air flow, comprising a bone marrow transplant unit (BMTU) and eleven naturally ventilated patient rooms of a haematology unit (HAEMU). Air samples were taken with an IDEAL air sampler (bioMerieux) on Sabouraud dextrose agar plates. The concentration of fungi in the air of the HEPA-filtered rooms of the BMTU ranged from 0-75 CFU/m3. Penicillium and Cladosporium were dominant among the fungal biota in the whole department. Of aspergilli, A. fumigatus was prevalent and seasonal increases in the frequencies of A. clavatus and A. niger isolation were observed. The detection of potentially pathogenic species of Aspergillus and Mucor in the BMTU and an increased concentration of Aspergillus in the HAEMU (up to 200 CFU/m3) instigated the introduction of additional preventive measure besides routine disinfection, namely an exchange of the HEPA filters in the BMTU and the installation of equipment based on multifunctional ion technology in the HAEMU. In a subsequent examination, a diminished number of fungi in the air was observed. During the study, 2 cases of proven and 3 of probable aspergillosis (according to EORT criteria) were noted. There was no link observed between the higher concentration of Aspergillus detected in the hospital air and the development of the infection. The authors conclude that hospital air examination can be helpful in indicating problems with hospital air facilities, enabling the introduction of procedures improving air quality and subsequently diminishing the risk of nosocomial mycoses.
An expert program has been developed for users working in industrial laboratories who are not experts in the identification of filamentous fungi. !e database of morphological growth features currently contains 12 species from the genera Aspergillus and Penicillium grown under standard conditions. !e identification algorithm implemented in the database takes into account the reliability of users, which can vary over a wide range depending on the identification feature. !e reliability of users was estimated on the basis of a questionnaire survey conducted among 27 non-experts, as the likelihood of a response consistent with the assessment of experts. The program works through comparative analysis of features of the fungus being identified with the expert-developed database and selection of the most likely species among the species represented by reference strains. !e expert program reduces subjective mistakes and may be extended to include further fungal species and genera; it can also be supplemented with chemotaxonomic, genetic and other data.
W pracy przebadano dwanaście szczepów grzybów strzępkowych z rodzaju Aspergillus, Trichoderma i Rhizopus pod względem zdolności do biosyntezy fitaz oraz enzymów towarzyszących tj. celulaz i ksylanaz, w hodowlach wstrząsanych oraz SSF, w obecności zmielonej fasoli, skrobi kukurydzianej, rzepaku oraz mąki sojowej jako źródeł węgla. Jedenaście szczepów było zdolnych do biosyntezy fitaz, wykazano jednak znaczne zróżnicowanie poziomu aktywności w zależności od źródła węgla w pożywce. Najefektywniejszym źródłem węgla do syntezy tych enzymów była zmielona fasola oraz skrobia kukurydziana. Największymi uzdolnieniami do biosyntezy fitaz charakteryzowały się szczepy Aspergillus niger 551 (34,56 nKat g⁻¹) oraz Aspergillus cervinus (31,85 nKat g⁻¹). W hodowlach prowadzonych w systemie SSF na zmielonej fasoli z udziałem obu szczepów uzyskano zwiększenie aktywności właściwej fitaz w porównaniu z hodowlą wgłębną. Enzymy towarzyszące, tj. celulazy i ksylanazy, w tych warunkach hodowlanych wytwarzane były na niskim poziomie.
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 6 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.