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Gastrointestinal parasites of free-range chickens

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The aim of the study was to determine the prevalence and intensity of parasitic gastrointestinal infections in free-range chickens from the West Pomerania province. Experimental material for the study was taken from 10 farms. Breeds raised in farms participating in the study included miniature chickens called Polish Lilliputians and Greenlegged Partridge. A total of 104 samples of faeces were examined. The Willis-Schlaff flotation method was used to assess the prevalence of infection, and McMaster’s method to evaluate the intensity. The presence of gastrointestinal parasites was found in 9 of the 10 farms. Oocysts of the genus Eimeria and eggs of gastrointestinal nematodes Ascaridia galli, Heterakis gallinarum and Trichostrongylus tenuis were isolated from the chicken faeces. Coccidiosis was found to be dominant parasitosis. The prevalence of infections on these farms with protozoa of Eimeria spp. was on average 32.7%, while for nematode species they amounted to 9.6% for Ascaridia galli, 5.7% for Heterakis gallinarum and 12.5% for Trichostrongylus tenuis. The results indicate the need to take preventive measures, designed to eliminate/reduce the risk of parasitoses in poultry from free-range systems. Focus should be placed on the hygiene of the farming conditions.
A case of parasite invasion was described in farmstead flock caused by Ascaridia galii, Capillaria spp., Heterakis gallinae and Trichostrongylus tenuis. Levamisole given twice at the rate of 40 mg per 1 kg of body weight at two week intervals proved to be highly effective against round-worms with the exception of Capillaria spp. whose eggs were noted 10 per cent of the faeces for three weeks after the treatment. The therapy prevented the broilers from dying, improved their general condition, body gains after the treatment, however were not equalized with that noted in healthy groups. Nine farms with 1300 hens were tested for Ascaridia galii. Among the farm under study only one was free from A. galii invasion. In the other eight farms, the rate of invasion ranged from 3.5 to 31 per cent and the intensiveness of the infestation was from 1 to 38 round-worms in a sample. The studies showed that broilers in farm­stead flocks were Infested by A. galii, as were adult hens on farms.
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Metody hodowli pasożytniczych nicieni in vitro

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The review of literature referring to cultivating methods of some parasitic nematodes in vitro is presented (Ascaridia galli, Ascaris suum, Nippostrongylus brasiliensis, Strongyloides fülleborni). It refers general problems and initiate in subject of parasites cultivating in vitro. This work comprises terminology and elementary problems connected with the starting of culture of parasites in vitro, such as: obtaining of parasites to the starting of cultures, the types of cultures in vitro and forming of conditions to determine successful of culture of parasitic nematodes in vitro (sterilization, the selecting of medium, the forming of most favourable physical-chemical conditions).
A group of 10-day-old chickens was experimentally infected with a dose of 500 infective eggs of Ascaridia galli (Schrank, 1788) (Nematoda). Forty-nine days post infection the chickens were necropsied and the infection intensily and prevalence were determined. The group maintained on the feed containing 11% of protein showed higher infection intensity and prevalence compared to the chickens fed a diet containing 19% of protein. The addition of vitamin B₂ to the feed containing 11% protein resulted in lower intensity and prevalence of the infection. In both series of experiments no effect of Zn-bacitracin or vitamin B₂ on infection intensity or prevalence was observed for chickens fed feed containing 19% of protein.
Hybrid chickens Astra S fed vitamin A deficiency diet containing 11 or 19% protein were infected with 500 invasive eggs of Ascaridia galli. One part of them received vitamin A in a dose corresponding to daily requirement, other part in double dose. After 7 weeks the extensity and intensity of ascaridiosis were determined. In pancreas and intestinal contents the activities of alpha-amylase, lipase and trypsin were measured. The invasion index was the highest in the group of chickens fed the protein and vitamin A deficiency diet. Vitamin A in a requirement dose lowered the parasite index. The results with double dose of vitamin A were worse in comparison with single dose. The activities of digestive enzymes were lower in infected animals than in the control groups. Vitamin A acted as moderator of the enzymatic changes during ascaridiosis.
The study was carried out on Astra S chickens which were grown on diets containing 11% and 19% proteins. In homogenized pancreas and duodenal contents from control animals and chickens infected with 500 invasive eggs of Ascaridia galli activities of alpha-amylase (FENNEL method), lipase (CHERRY-CRANDALL method) and trypsin (ANSON method) were determined. After 7 weeks of the invasion the activities of these enzymes were higher in duodenal contents and lower in pancreas of infected birds in comparison with the control animals. The differences were significant for alpha-amylase and lipase activities in animals which were given 11% protein diet, and for trypsin activity of chickens groups fed with 19% protein diet.
The studies were carried out on the chickens given the diet containing 19% and 11% of protein. On the 7th day of their life one part of the chickens were infected with different doses (125-500) of invasive eggs of Ascaridia galli. The other part of the birds were infected with 500 eggs of A. galli and the invasion lasted from 5 to 49 days. In the extracts from pancreas the activities of trypsin and α-amylase were measured. No differences were observed between trypsin activity of control and infected chickens fed with diet containing 11% of protein. The activity of trypsin was lower in infected chickens fed with diet containing 19% of protein, when the invasion lasted over 15 days. The activity of α-amylase in both groups decreased after infection with more than 100 invasive eggs of A. galli. The intensity and the extensity of invasion were lower in the chickens given the diet of 19% of protein.
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