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A methanolic extract of Artemisia annua was obtained to evaluate its insecticidal activities against the sunn pest (Eurygaster intefriceps). Also, the responses of general esterase (EST), glutathione S-transferase (GST), alkaline phosphatase (ALP), acid phosphatase (ACP), acethylcholinesterase (AChE) to the plant extract were investigated. Topical application of plant extract on adults showed that the mortality was dose-dependent i.e. with increasing of plant extract concentrations more mortality achieved. Esterase and GST activities were increased in the first 24 h post-treatment. However, the enzymes activities were decreased after 24 h until 72 h. The activities of ALP, ACP and AChE in insect body decreased significantly and inhibition was higher along with increasing concentrations of plant extract. Isozyme electrophoresis profiles indicated that responses of isozymes (EST and GST) to plant extract were decreased after 48 h exposure to extract so that some enzymes bands disappeared. The results indicated that the highest concentration of A. annua extract was the most toxic among the four extracts. The decline of the detoxification ability in insects' tissues might be the main reason for the insecticidal activities.
Toxicity and physiological alterations were determined in Pseudococcus viburni nymphs treated with Artemisia annua methanolic extract. The leaf dipping bioassay showed LC50 values of 0.287% and 0.194% 24 and 48 hours post-exposure. Activities of general esterases were significantly higher in the control nymphs than in those which had been treated except for the 48 h time interval using α-naphtyl acetate. The activity of glutathione S-transferase using CDNB (1-chloro-2,4-dinitrobenzene) in the control nymphs, was significantly higher than in the control at both time intervals while no significant difference was observed after 24 h in addition to the higher enzymatic activity in the treated nymphs after 48 h. All three aminotransferases were significantly more active in the control nymphs except for time intervals of 24 h for γ-glutamyl transferase and 48 h for alanine aminotransferase. Higher activities of lactate dehydrogenase, acid- and alkaline phosphatase were found in the control nymphs than in treated nymphs for all time intervals. Activities of the enzymes involved in the antioxidant system including catalase, peroxidase, superoxide dismutase, ascorbate peroxidase and glucose-6-phosphate dehydrogenase was increased in the treated nymphs compared to the control. Results of the current study demonstrated toxic effects of A. annua methanolic extract on P. viburni nymphs causing mortality and physiological turbulences.
Essential oil of Artemisia annua L. was investigated to find out its toxicity and physiological aspects on the slug Agriolimax agrestis, in controlled conditions (8±1°C, 75±5 RH and 14:10 LD). The slugs received different concentrations of essential oil treated radish leaves in methanol, while the control received methanol alone. LC10, LC30, LC50 and LC90 values were estimated at 4.67, 5.3, 5.81, 7.25%, respectively. The effect of the essential oil on some important enzymatic components like; cytochrome P450 monnooxygenase, acid phosphatase, alkaline phosphatase, lipase, amylase and protease were significantly increased compared to the control. These results indicate that the plant Artemisia annua L. not only shows toxicity but also shows some irreversible effect on some important biochemical components and deserves further investigation.
Two new for Wrocław localities of Artemisia annua L., the species not recognised since 1976 in this city, has been found. The plant grew on a sack with sand, on railway and on a new sidewalk near a church. Formerly it was reported within Wrocław city limits several times, usually from rubbish. This paper presents short description of this species, its ecological and climatic requirements, information of geographical distribution in the world and in Europe, medical importance, capacity for expansion and an issue of cultivation.
The chemical composition of worm wood Artemisia annua L. (Astraceae), a medicinal plant, was studied by gas chromatography and gas chromatographymass spectrometry (GC-MS). About 60 compounds were identified, from which the major compounds were erythritol (50.30%), camphore (7.25%), pinocarveol (4.13%) and diethoxyethane (2.18%). The effect of crude extract on deterrency was maximal in 1000 μL/L treatment (only 4.66 ±1.05 insects were attracted to treated food) compared to control (12.6 ±0.96) The growth regulatory effect showed that 1600 μL/L treatment variously affected the weight of larvae, pupae and adult (0.48 ±0.003, 2.17 ±0.02 and 1.31 ±0.015, respectively) and finally the ovicidal effect and the number of adults appearing in F1 were similarly affected (38.5 ±1.7 and 33.75 ±2.31), comparing to control (99.25 ±1.727 and 92.5 ±1.35). The present result and previous results on this plant species indicate that it can be a good candidate for controlling stored product insects.
Methanolic extract of Artemisia annua L. were evaluated on growth and nutritional efficiency of lesser mulberry pyralid Glyphodes pyloalis (Lepidoptera: Pyralidae) in laboratory conditions (24±1°C, 75±5% RH and 16 L : 8 D). Feeding deterrence index was evaluated by a choice test method on < 24 h fifth instar larvae with 5, 2.5, 1.25 and 0.625 percent of the extract. The result indicated that increasing the concentration resulted in higher deterrence. The extract retarded larval growth significantly (EC50 = 3.63%) and also showed significant effects on the relative growth rate (RGR), efficiency of conversion of ingested food (ECI), efficiency of conversion of digested food (ECD), approximate digestibility (AD) and consumption index (CI). Lipid, protein and carbohydrate in treated insects were significantly reduced compared to the controls. Hence, methanol extract of A. annua could be considered as a strong deterrent which also affects the biochemical metabolism of the target pest. Methanol extract of A. annua has the potential for development as a botanical insecticide.
This study evaluated the effects of addition of lucerne extract (LE), Artemisiae annuae extract (AAE), and mixed herbal medicine (MHM) into different goat diets on in vitro rumen fermentation and methane production. In addition to the negative control (NC), addition of monensin (MO) served as the positive control (PC). Four ruminally cannulated Nanjiang Yellow goats (45 ± 2 kg liveweight) were used as donors of ruminal fluid. The results showed: 1. compared with NC, addition of all the additives into the mixed grass diet increased propionate concentration (P<0.05). Protozoa numbers and methane production were reduced by addition of all the additives (P<0.05); 2. compared with NC, LE and AAE in the lucerne diet increased propionate concentration (P<0.05). All the additives reduced protozoa numbers (P<0.05). Methane production was decreased by addition of LE compared with NC and PC (P<0.05); 3. addition of LE into the mixed grass-concentrate diet resulted in an increase of the propionate concentration compared with NC and PC (P<0.05). Protozoa numbers were reduced by addition of LE and AAE compared with NC (P<0.05). Methane production was decreased by addition of AAE compared with NC and PC (P<0.05); 4. compared with NC, addition of AAE and MHM into the lucerne-concentrate diet resulted in an increase of propionate concentration (P<0.05). Protozoa numbers were reduced by addition of all the additives (P<0.05). Methane production was decreased by addition of AAE and MHM (P<0.05). In conclusion, addition of LE, AAE and MHM into different diets reduced methane production, increased propionate concentration and decreased protozoa numbers to a certain extent, and the inhibitory effects of the phytogenic products on methane production are more remarkable in the mixed-grass diet. The phytogenic products appear to be promising alternatives to MO in altering in vitro rumen fermentation and reducing methane production in goats.
The essential oil of Artemisia annua L., a weed collected from northern Iran, was studied for its toxicity and physiological aspects on 4th instar larva of the cotton bollworm Helicoverpa armigera Hübner in controlled conditions (26±1°C, 65±10% RH and 16 L : : 8 D h). The artificial diet was used as a medium for investigating the toxicity and the effect of LC10, LC30, LC50, and LC90 on the feeding efficiency of 4th instar larva. The essential oil in doses of LC10, LC30, LC50, and LC90 were estimated to be 2.01%, 3.86%, 6.07%, and 18.34%, respectively. The activity of α-amylase, protease, lipase, general esterases, and glutathione S-transferase and protein, triglyceride, glucose for treated larva were measured. The results showed that all of these parameters were decreased compared with the control. Hence, A. annua essential oil is suggested as a botanical for controlling this important pest of field crops.
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