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Author proposes short squash method with orcein and Allium sp., practical in didactics especially and gives some remarks as for karyological observations.
Allium olivieri Boiss. (Alliaceae) is reported as a new record for Turkish flora. It was identified using the collected specimens and Flora orientalis, Flora of Iraq and Flora Iranica. The detailed description, illustrations, geographical distribution, habitat, status of IUCN extinction risk and some comments and discussions on the taxonomy of the species are given.
The aim of the present work was to identify GCL, OYD and LYS and to assess the degree of their distribution in crop and ornamental plants from the genus Allium. Two groups of plants from the genus Allium were used. The first group included 10 botanical species, while the second group was composed of seven commercial A. sativum cultivars and two genotypes. Identification of GCL, OYD and LYS in leaves, inflorescences, and bulbs was performed with the use of the ELISA test. All plants in the first group consisting of botanical species of the genus Allium were free from the viruses studied, whereas in commercial A. sativum cultivars a high prevalence of GCLV, OYDV and LYSV infection reaching 88.2%, 75% and 32.1%, respectively, was reported. Varying severity of infection in the particular plant organs was found.
Studies carried out in 2003–2005 included determinations of free phenolic acids content in edible parts (shoots, pseudostem) of onion, shallot, and Welsh onion grown for green bunching in the field and forced in the greenhouse. Plants for phenolic acids contents analyses were achieved from setting the small bulbs (common onion, shallot) or annual plants from the seedling setting in the case of Welsh onion. In the field cultivation, plants were grown under short-term covers made of perforated PE film and non-woven PP, while forcing was realized in heated and unheated greenhouse. Free phenolic acids contents were determined after plant harvest, when plants reached the size useful for trading in bunches. Phenolic acids contents were determined in shoots and pseudostem by means of spectrometric Arnova method with recalculation onto caffeic acid. Performed study revealed that shoots contained significantly more free phenolic acids as compared to the pseudostem. Referring to the field cultivation, the component concentration in shoots was 0.23 mg·100 g⁻¹ FW, whereas due to forcing 0.135 mg·100 g⁻¹ FW, on average. Contents of phenolic acids at pseudostems of studied plants from the field cultivation were 0.05 mg·100 g⁻¹ FW, while at forced plants 0.04 mg·100 g⁻¹ FW. No significant differences related to the level of phenolic acids between common onion and shallot were found, both in shoots and pseudostem. However, shoots of Welsh onion contained significantly less phenolic acids, regardless the cultivation place and cover application. The accelerated field cultivation resulted in shoots of Welsh onion was 0.19 mg·100 g⁻¹ FW phenolic acids in FW, whereas the greenhouse forcing 0.11 mg·100 g⁻¹ FW. Regardless the species, forced plants contained considerably less phenolic acids (namely in shoots) as compared to plants grown in the field.
Garlic virus A (GarV-A), Garlic virus B (GarV-B), Garlic virus C (GarV-C) and Garlic virus X (GarV-X) are members of the genus Allexivirus in the family Alphaflexiviridae. In this study, we collected 10, 30, 10 and 14 isolates of GarV-A, GarV-B, GarV-C and GarV-X, respectively, from different parts of Poland. All sequences of coat protein (CP) and nucleic-acid binding protein (NABP) regions of Allexivirus isolates available in GenBank were also included in this study. The nucleotide and amino acid sequences identities within each population differed substantially depending on the region of the genome and virus species. The results of selection pressure analysis showed that populations of each Allexivirus underwent negative selection, but the extent of the negative selection varied. It was also concluded that the GarV-A and GarV-C populations underwent a decrease in population size or balancing selection, while the GarV-B and GarV-X populations underwent an increase in population size. It was concluded that both populations of GarV-X evolved independently in each respective area, in contrast to populations of GarV-A, GarV-B and GarV-C.
The pollen morphology of eight species from three subgenera and five sections of the genus Allium L. was studied by LM and SEM (i.e.: A. angulosum, A. carinatum, A. senescens subsp. montanum, A. oleraceum, A. scorodoprasum, A. ursinum, A. victorialis and A. vineale). The material came from natural sites of these species located in Poland, Czech Republic, Austria and Italy. For measurements a sample consisted of 30 pollen grains. In total, 240 pollen grains were analysed. They were analysed in respect to six quantitative features (i.e.: length of long axis – LA, length of short axis – SA, thickness of exine along long axis – Ex, SA/LA and Ex/LA ratios and length of sulcus) and the following qualitative ones: pollen outline and shape, exine ornamentation. Taxonomic value of these pollen features is considerable, especially on the sections level. On the basis of these features, it is impossible to distinguish individual Allium species but only their groups. The examined features were characterized by moderate (LA, SA and SA/LA) or high variability (Ex, Ex/LA). Among studied species the lowest variability was found in A. victorialis and the highest in A. vineale and A. oleraceum.
This study was conducted in the years 1997-1999. From the collection of the UMCS Botanical Garden, nine species of garlic were selected (A. aflatunense, A. atropurpureum, A. caeruleum, A. cernuum, A. ledebourianum, A. lineare, A. sphaerocephalon, A. victorialis, A. ursinum) and one subspecies (A. scorodoprasum subsp. jajlde). Pollen grain viability was evaluated on microscopic slides stained with acetocarmine, germination ability on the agar medium and measurements of grains were made on glycerin jelly slides. The studied species were characterized by high pollen viability (87-99%) what indicates the great value of garlic flowers as a source of protein-rich feed for honey-bee and wild pollinating insects. Very low germination of pollen on the agar medium was recorded. The length of the equatorial longitudinal axis ranged from 24.3 µm to 37.5 µm and it allowed pollen of most garlic species to be classified as medium-sized grains and only the pollen of A. caeruleum and A. cernuum was included in the group of small-sized grains.
Observations on the species composition of ground beetles (Coleoptera, Carabidae) were conducted in the years 2008–2009 in the collection of plants of the genus Allium spp. belonging to Laboratory of Plant Genetic Resources in the Skierniewice IW (UTM: DC 45). Ground beetles were collected using the Barber’s soil traps. In the first year of the study were captured a total of 1 277 adults of Carabidae, in the next year 1 197 ones. The most numerous species trapped were: Bembidion properans (Stephens), B. quadrimaculatum (Linnaeus), Harpalus affinis (Schrank).
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