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Low level of genetic variation within Melica transsilvanica populations from the Krakow-Czestochowa Upland and the Pieniny Mts revealed by AFLPs analysis

100%

Szczepaniak M., Cieslak E.

Acta Societatis Botanicorum Poloniae

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2007

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tom 76

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nr 4

321-331

Fragmented distribution, the breeding system and effects of genetic drift in small-size populations occurring at edge of the species range play an important role in shaping genetic diversity of such a species. Melica transsilvanica is a plant rare in the flora of Poland, where it reaches the northern limit of its continuous range. Amplified Fragment Length Polymorphism (AFLP) DNA profiling method was applied to measure genetic diversity among and within populations of M. transsilvanica. Additionally, genetic relationships between M. transsilvanica and Melica ciliata, two closely related species, were explored. A total of 68 plants from 7 populations of M. transsilvanica and 24 plants from 2 populations of M. ciliata, collected in Poland and outside it, were analyzed. Using 294 AFLP fragments from 3 primer combinations, accessions were grouped into two major clusters associating with M. ciliata and M. transsilvanica, respectively. Further, two subclusters, corresponding to the samples collected from the Pieniny Mts and from the Kraków - Częstochowa Upland were clearly distinguished within the M. transsilvanica group. The hierarchical AMOVA exhibited significant genetic distinction between these geographic regions (60.89%, p < 0.001). The obtained results showed that the most genetic diversity resided between the populations of M. transsilvanica (86.03%) while considerably lower genetic variation was found within the populations (13.97%), which is consistent with the results reported for self-plants. The low level of AFLP genetic variation of M. transsilvanica can be caused by the geographic isolation of populations, which preserves the dominant self-mating breeding system of the species. Individual populations of M. transsilvanica are characterized by isolated gene pools differing by a small number of loci.

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Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions

100%

Skibinska M., Kosmala A., Humphreys M. W., Zwierzykowski Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 2A

At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid - L. multiflorum (4x) x F. pratensis (2x) and pentaploid - F. arundinacea (6x) x L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented.

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Zastosowanie markerow AFLP do badania zmiennosci genetycznej w obrebie form rodzicielskich mieszanca heterozyjnego zyta otrzymanego na bazie cytoplazmy PAMPA

70%

Bednarek P. T., Kubicka H., Zawada M., Brukwinski W.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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1999

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nr 211

229-237

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Wykorzystanie markerow genetycznych typu AFLP do badan zmiennosci genetycznej linii wsobnych zyta

70%

Bednarek P. T., Chwedorzewska K., Kroliczak J., Puchalski J., Zawada M.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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1999

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nr 211

219-227

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Proba wykorzystania techniki AFLP do oceny zmian genetycznych zachodzacych podczas dlugotrwalego przechowywania i regeneracji zyta [Secale cereale L.]

61%

Chwedorzewska K., Bednarek P. T., Puchalski J.

Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

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2000

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tom 216

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nr 1

111-116

Ograniczanie wyników

3 Biuletyn Instytutu Hodowli i Aklimatyzacji Roślin

1 Acta Societatis Botanicorum Poloniae

1 Cellular and Molecular Biology Letters

3 Bednarek P. T.

2 Chwedorzewska K.

2 Puchalski J.

2 Zawada M.

1 Brukwinski W.

1 Cieslak E.

1 Humphreys M. W.

1 Kosmala A.

1 Kroliczak J.

1 Kubicka H.

1 Skibinska M.

1 Szczepaniak M.

1 Zwierzykowski Z.

1 2007

1 2002

1 2000

2 1999

Low level of genetic variation within Melica transsilvanica populations from the Krakow-Czestochowa Upland and the Pieniny Mts revealed by AFLPs analysis

Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions

Zastosowanie markerow AFLP do badania zmiennosci genetycznej w obrebie form rodzicielskich mieszanca heterozyjnego zyta otrzymanego na bazie cytoplazmy PAMPA

Wykorzystanie markerow genetycznych typu AFLP do badan zmiennosci genetycznej linii wsobnych zyta

Proba wykorzystania techniki AFLP do oceny zmian genetycznych zachodzacych podczas dlugotrwalego przechowywania i regeneracji zyta [Secale cereale L.]