Pyrenophora tritici-repentis, anamorph Drechslera tritici-repentis, strains used in this study represented three phenotypic c1asses of this fungus: the wild type, strains unable to produce melanins - mel(-), and intermediate strains. Using an HPLC system with a reversed phase Cl18 column synthesis of anthraquinone pigments by strains belonging to all three classes could be detected. According to the 'H NMR spectra analysis the major compounds produced by P. tritici-repentis are catenarin, helminthosporin, islandicin, emodin, ω-hydroxy-islandicin, ω-hydroxy-catenarin and ω-hydroxy-helminthosporin. However, amounts of these compounds produced by the wild type were up to 350 times lower than those observed for the mel (-) forms. Some differences in profiles of these compounds, produced by different strains were also observed.
Podatność pszenżyta na porażenie przez Puccinia striiformis, Puccinia graminis i Puccinia recondita badano w doświadczeniach polowych. Oceniano materiał hodowlany pszenżyta ozimego i jarego z 5 krajowych ośrodków hodowli z różnych rejonów Polski. Materiały hodowlane pszenżyta okazały się stosunkowo wrażliwe na porażenie przez Puccinia striiformis. Na 250 testowanych genotypów pszenżyta tylko 51 okazało się odpornych na rdzę żółtą. Wśród przebadanych 250 odmian i rodów 181 genotypów okazało się odpornych na porażenie przez Puccinia graminis. W równoległych doświadczeniach z Puccinia recondita aż 136 genotypów (na 250) było odpornych na rdzę brunatną. Procentowy udział form odpornych wśród rodów pszenżyta ozimego był wyższy niż w przypadku pszenżyta jarego.
Valdensia heterodoxa as a parasitic fungus was observed on Ericaceae family plants i.e. blueberry (Vaccinium corymbosum L.), bilberry (V. myrtillus L.) and lingonberry (V. vitis-idaea L.). Isolates of this polyphagous were obtained from the infected leaves of different cultivars of highbush blueberry collected from commercial plantation in Mazovia province and from bilberry collected from forests in Pomerania province. PCR amplification of selected rDNA fragments (ITS1, 5.8S, ITS2) was done with ITS1F and ITS4A primers. Bioinformatic analysis revealed similarity 99–100% between selected nucleotide sequences of V. heterodoxa isolates from bilberry and highbush blueberry. The sequences of bilberry isolates were obtained and described for the first time in Poland. Their reference sequence was deposited in GenBank (KT121733). In laboratory experiments conidia of selected bilberry isolates on OA medium were 278 ±6 × 140 ±4 μm. Conidia from highbush blueberry, bilberry, and lingonberry were measured. Depending on the host plant conidia were different in the length of the arms and width of the head. The growth of the fungal isolates on PDA (potato dextrose agar), OA (oatmeal agar), WOA (weak oatmeal agar), SNA (salt nutrient agar) media was examined. The cultures were divided into two groups based on their morphology on PDA medium.