Wyniki wyszukiwania

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Biological threat detection in the air and on the surface: how to define the risk

100%

Trafny E. A., Lewandowski R., Stepinska M., Kaliszewski M.

Archivum Immunologiae et Therapiae Experimentalis

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2014

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tom 62

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nr 4

2

Rapid and reliable detection of Echinococcus multilocularis from faeces using droplet digital PCR

86%

Bago F., Hoelzl F., Knauer F., Kubber-Heiss A., Smith S.

Acta Parasitologica

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2021

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tom 66

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nr 2

3

A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

72%

Zhang S.-J., Wang L.-L., Lu S.-Y., Hu P., Li Y.-S., Zhang Y., Chang H.-Z., Zhai F.-F., Liu Z.-S., Li Z.-H., Ren H.-L.

Journal of Veterinary Research

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2020

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tom 64

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nr 2

4

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Rapid and specific detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation combined with loop-mediated isothermal amplification

72%

Qin Y., Puthiyakunnon S., Zhang Y., Wu X., Boddu S., Luo B., Fan H.

Polish Journal of Food and Nutrition Sciences

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2018

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tom 68

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nr 2

Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplifi cation (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with antiEHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identifi ed by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specific gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specificity and sensitivity, with a positive detection rate of amplifi cation of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 101 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×101 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specific gene amplifi cation technology that is suitable for implementing as a screening assay in basic laboratory and field test for detecting food contamination.

5

HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA

72%

Osinska E., Golke A., Slonska A., Cymerys J., Banbura M. W., Dzieciatkowski T.

Polish Journal of Veterinary Sciences

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2012

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tom 15

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nr 3

Equid herpesvirus type 2 (EHV-2) together with equid herpesvirus type 5 are members of Gammaherpesvirinae subfamily, genus Rhadinovirus. EHV-2 is one of major agents causing diseases of horses common worldwide. A possible role of EHV-2 in reactivating latent equid herpesvirus type-1 has been suggested, because reactivation of latent EHV-1 was always accompanied by EHV-2 replication. Variety techniques, including cell culture, PCR and its modifications, have been used to diagnose EHV-2 infections. The aim of this study was to develop, optimize and determine specificity of real-time PCR (qPCR) for EHV-2 DNA detection using HybProbesR chemistry and to evaluate clinical samples with this method. The analytical sensitivity of assay was tested using serial dilutions of viral DNA in range between 70 and 7x105 copies/ml. The limit of detection (LOD) was calculated using probit analysis and was determined as 56 copies/ml. In further studies 20 different clinical samples were tested for the presence of EHV-2. Described in-house qPCR method detected viral DNA in 5 of 20 specimens used. The results of this work show that developed HybProbes-based real-time PCR assay is very reliable and valuable for detection and quantification of equid herpesvirus type 2 DNA in different clinical samples. The high level of sensitivity, accuracy and rapidity provided by the LightCycler 2.0 instrument are favorable for the use of this system in the detection of EHV-2 DNA in veterinary virology.

Ograniczanie wyników

Biological threat detection in the air and on the surface: how to define the risk

Rapid and reliable detection of Echinococcus multilocularis from faeces using droplet digital PCR

A novel, rapid, and simple PMA-qPCR method for detection and counting of viable Brucella organisms

Rapid and specific detection of Escherichia coli O157:H7 in ground beef using immunomagnetic separation combined with loop-mediated isothermal amplification

HybProbes-based real-time PCR assay for rapid detection of equine herpesvirus type 2 DNA