Recent reports indicate that mini chromosomes and other small genetic entities may occur in the nuclei of uninfected higher plants. They become especially abundant under some special growth conditions and, sometimes, resemble extrachromosomal genes of ciliated protozoa. An example of such a gene-sized DNA species was isolated from resting wheat embryos. Hie presence of telo- meric sequences at its termini and the ability to replicate autonomously in wheat nuclei made it possible to distinguish this nuclear mini chromosome from chromosomal DNA fragmentation products. The biological significance of plant mini chromosomes remains to be elucidated.