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Modulated human globin gene expression: role for antisense expression vectors

100%

Pace B. S.

Cellular and Molecular Biology Letters

1998

tom 03

nr 2

Modulated human globin gene expression: role for antisense expression vectors

84%

Xu L., Pace B. S.

Cellular and Molecular Biology Letters

1998

tom 03

nr 4

Several approaches have been explored to prevent polymerization of sickle hemoglobin in erythrocytes. We tested the ability of a mammalian expression vector carrying a β gene antisense cDNA fragment to block β gene expression. The antisense expression vector was stably transfected into HS2γβ stable mouse erythroleukemia cell lines producing human γ and β globin chains. By day 14 there was an average 24% decrease and 12% increase in β and γ globin mRNA levels respectively. We observed a loss of β gene inhibition by day 42. This study suggests that a β globin antisense cDNA expression vector may be an alternative gene therapy strategy to decrease sickle hemoglobin levels in patient with sickle cell disease.

Ograniczanie wyników

2 Cellular and Molecular Biology Letters

2 Pace B. S.

1 Xu L.

2 1998

Wyniki wyszukiwania

Modulated human globin gene expression: role for antisense expression vectors

Modulated human globin gene expression: role for antisense expression vectors