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In vitro mass propagation of Withania somnifera Dunal using seaweed extract

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Sathees Kannan T. M., Sownthariya S., Anbazhakan S.

International Letters of Natural Sciences

2014

tom 19

The present study was aimed to develop a cost-effective and efficient protocol for mass propagation of high-quality seedlings through tissue culture by using seaweed extract as biostimulants instead of synthetic chemicals. The nodal explant of field grown W. somnifera estabilised on Murashige and Skoogs medium (MS) and Gamborg B5 medium supplemented with six concentration of 2,4 D. The percentage of culture response from the nodal explant ranged from 44 to 80 and 3.0 mg l-1 2,4 D found to be best for callus induction. MS media containing different concentration of seaweed extract (10, 20, 40, 60, 80 and 100 %) were tested individually for shoot induction. The medium supplemented with 40 % seaweed extract exhibited maximum number of shoots with about 8.6 shoots/ callus and 80 % seaweed extract exhibited 4.3 shoots/ callus. It is evident from this study that seaweed extracts can be used as substitute for synthetic growth hormones for micropropagation of medicinally important plant W. somnifera for clonal propagation and conservation.

Evaluation of bio control efficacy of synbiotic cherry ( Prunus avium. L.,) juice

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Sheela T., Sathees Kannan T. M., Jayakumar K.

International Letters of Natural Sciences

2015

tom 42

The aim of the research was to evaluate that the effect of symbiotic fermented cherry juice containing fructo oligosaccharide to enhance the growth and activity of probiotic strains include Lactobacillus acidophilus was tested for their antibiotic susceptibility, and tolerance to bile. Antifungal activity of symbiotic cherry juice could differ in their antagonistic activity against fungal disease which could be due to the metabolite secreted by the lactic acid bacteriocin specially type of organic acids and added fructo oligo saccharide as a probiotic and for food preservation synbiotic cherry juice was identified and their major compounds was detected using gas chromatographymass spectrum (gc-ms).

Ograniczanie wyników

2 International Letters of Natural Sciences

2 Sathees Kannan T. M.

1 Anbazhakan S.

1 Jayakumar K.

1 Sheela T.

1 Sownthariya S.

1 2015

1 2014

Wyniki wyszukiwania

In vitro mass propagation of Withania somnifera Dunal using seaweed extract

Evaluation of bio control efficacy of synbiotic cherry ( Prunus avium. L.,) juice