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Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliabeta-1,3-glucanase genes in transgenic potato plants

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Moravcikova J., Libantova J., Hedlak J., Salaj J., Bauer M., Matusikova I., Galova Z., Mlynarova L.

Acta Physiologiae Plantarum

2007

tom 29

nr 2

The genes encoding for a cucumber class III chitinase and Nicotiana plumbaginifolia class I glucanase were co-introduced into Slovak potato (Solanum tuberosum L.) cultivar ETA using Agrobacterium tumefaciens. Expression of both genes was driven by wound-inducible polyubiquitin promoter isolated from Slovak potato breeding line 116/86. Analyses showed inducible, peel-specific expression of both transgenes under stress conditions. The effect of transgene expression on fungal susceptibility of transformants was evaluated in vitro and in vivo. Experiments with crude protein extracts isolated from transgenic microtubers showed growth inhibition of Rhizoctonia solani hyphae in the range from 7.3 to 14.2%. In contrast, experiments performed in growth chamber conditions revealed that the polyubiquitin promoter driven transgene expression did not ensure any obvious increase of transgenic potato resistance against Rhizoctonia solani.

Ograniczanie wyników

1 Acta Physiologiae Plantarum

1 Bauer M.

1 Galova Z.

1 Hedlak J.

1 Libantova J.

1 Matusikova I.

1 Mlynarova L.

1 Moravcikova J.

1 Salaj J.

1 2007

Wyniki wyszukiwania

Stress-induced expression of cucumber chitinase and Nicotiana plumbaginifoliabeta-1,3-glucanase genes in transgenic potato plants