Wyniki wyszukiwania - AGRO

1

On the climatic uncertainty to the environment extremes: a Singapore case and statistical approach

100%

Zhang Y.

Polish Journal of Environmental Studies

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2015

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tom 24

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nr 3

In recent times, increasing economic losses due to unexpected extreme environmental conditions have been noticed. The occurrence of these extreme environmental events requires us to have a clearer understanding of climatic influence on our lives. This paper utilizes the case of Singapore to investigate the influence of climate extremes on a country. The environmental elements of Singapore whose extremes are most likely to change in the future are identified. The impacts of these extremes on an entire nation’s economy and public health are discussed. Additionally, climate-induced statistical uncertainties in the modeling of extreme values are investigated. Based on the available knowledge of climate uncertainty information, the change of statistical structure in the extreme value model is discussed. The impact of various types of climate uncertainty in the extreme value modeling for a physical environmental variable is summarized at the end of this paper.

2

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Caspase-1 inhibitor reduced the lung injury in a mouse model of pleuropneumonia caused by Actinobacillus pleuropneumoniae

72%

Zhang Y., Yang T., Huang F., Zhang Y.

Polish Journal of Veterinary Sciences

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2020

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tom 23

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nr 4

3

Effects of experimental warming and CO2 concentration doubling on 13C CPMAS NMR spectra of humin in coniferous forest ecosystems of the eastern Tibetan Plateau in China

64%

Liu F., Zhang Y.

Polish Journal of Environmental Studies

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2019

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tom 28

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nr 4

Despite the perceived importance of humin to forest ecosystem C sequestration, few studies have attempted to examine the responses to elevated temperature (ET, +2.5ºC) and elevated atmospheric CO2 concentration (EC, 700 ppm) on the chemical structures of humin. Solid-state 13C cross-polarization magic angle spinning nuclear magnetic resonance (13C CPMAS NMR) spectroscopy was applied to evaluate the 6-year effect of ET and EC on the chemical structure of humin under the coniferous forest ecosystems in the eastern Tibetan Plateau of China. Results showed that ET treatment decreased soil organic carbon (SOC), whereas EC and ETC treatment increased SOC. ET treatment decreased aromatic C and carbonyl C of humin while increasing alkyl C, the ratios of alkyl C/O-alkyl C, aliphatic C/aromatic C, and hydrophobic C/hydrophilic C. Compared with ET treatment, the ETC treatment had a similar but reduced impact on the chemical structure of humin, while EC had slightly and undetectable impact on the chemical structure of humin. The 6-year exposure to ET decreased SOC and changed the molecular structure of soil humin to be more alkyline and hydrophobic, and it was a protection mechanism to the sequestration and stability of organic C in soil.

4

Identification of heat-stable proteins in imbibed Cowpea (Vigna unguiculata) seeds

64%

Zhang Y., Li Y.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 02

Heat-stable protein fraction in seeds is believed to enrich many proteins functioning in the acquisition of stress-tolerance of seeds. In this study, the composition of heat-stable protein fraction in imbibed cowpea (Vigna unguiculata) seeds was analyzed by SDS-PAGE and twodimensional gel electrophoresis coupled with mass spectrometry. The results indicated that approximately 12.4 % of seed soluble proteins were stable after heat treatment at 100 C for 10 min. Twenty-two putative heat-stable proteins were identified using MALDI-TOF/TOF MS. Most of these heat-stable proteins were late embryogenesis abundant proteins, and there were other stress-related proteins including Cu/Zn superoxide dismutase and 17.4 kDa Class I heat-shock protein. A cyclophilin protein, a cleavage and polyadenylation specificity factor and a Pumilio-family RNA binding protein were also present in the heat-stable fraction. The identified heat-stable proteins were more hydrophilic proteins and may accumulate to stabilize cellular components and maintain seed viability during seed development and germination.

5

Energy use and carbon emissions efficiency study of chinese regions based on price factor

64%

Huang Y., Zhang Y.

Polish Journal of Environmental Studies

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2018

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tom 27

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nr 5

As China has committed to the international community to effectively control CO₂ emissions, it is necessary for the regions of China to launch an in-depth analysis about energy use and CO₂ emission efficiency. In this paper, each province of China will be regarded as an independent decision-making unit. After judging their return to scale state by the traditional DEA theoretical model, we use the SBM and RE/CE models, which respectively solve the problems of slack variables, and the traditional model does not reflect factors such as price, which is truly existing. Then we get a more comprehensive efficiency that reveals Chinese energy use and the CO₂ emissions situation. From the empirical study of 30 regions in China, we know that the southern region of China has the most efficient score while northeastern China has poor performance. Price factor has a significant influence on energy use and CO₂ emissions efficient score of some provinces. Our study shows that northern and northeastern China should make more of an effort on energy consumption reduction in order to improve the efficiency score. Instead, northeastern and central China should pay more attention improving energy conversion technology in order to increase their efficiency.

6

Subjective mental workload measures

64%

Zhang Y., Luximon A.

Ergonomia

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2005

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tom 27

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nr 3

7

Differential accumulation of proteins in leaves and roots associated with heat tolerance in two Kentucky bluegrass genotypes differing in heat tolerance

64%

Zhang Y., Du H.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 09

8

Research on the carbon emissions of beijing residents based on the Input-output model

64%

Zhang Y., Lei Y.

Polish Journal of Environmental Studies

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2017

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tom 26

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nr 5

China is facing increasing pressure on international emissions. As the capital of China, Beijing should set an example for carbon emissions. Previous studies on carbon emissions mainly focused on household size and changes in household consumption structure during urbanization. The input-output method is mainly used, but the total output of each department is used to measure the intensity of CO₂ emissions, so that the middle input and added value among the sectors are included, which leads to a large measurement result. Based on the input-output model, the paper chooses the latest input-output table of Beijing in 2012 and calculates the carbon emissions of residents in Beijing in 2012, which calculates the CO₂ emission intensity by using the added value that avoids the problem of double counting. The results show that the total carbon emissions of Beijing residents is 762.948 million tons, of which the direct consumption energy of urban residents is mainly gasoline and heat, while that of rural residents is mainly coal and electricity. Indirect carbon emissions of residential consumption are divided into eight consumption categories. Among them, 57.2% and 18.9% were in transportation and housing, respectively. According to the above conclusions, this paper makes some recommendations.

9

Biological reduction of hydrogel-encapsulated Fe(III) by Shewanella oneidensis MR-1: incubation experiment and kinetic modeling

64%

Zhang Y., Li H.

Polish Journal of Environmental Studies

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2016

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tom 25

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nr 2

Dissimilatory Fe(III) reduction has a profound infl uence on the global cycling of elements and the decontamination of pollutants, depending on the interaction of various environmental conditions. Hydrogelencapsulated goethite/soil was prepared in this study, and anaerobic incubation was conducted to investigate the biological Fe reduction of different encapsulated aggregates by Shewanella oneidensis MR-1. Results indicated that the release of Fe(II) ion was signifi cant and insignifi cant in R-soil treatments with and without anthraquinone-2,6-disulfonate (AQDS), respectively. The increase in the cross-linker ratio in the hydrogel decreased iron reduction. The Fe(II) concentration followed the order of unencapsulated treatment < 0.3% encapsulated hydrogel treatment < 2% encapsulated hydrogel treatment with AQDS addition. The results of the goethite experiment suggested that the goethite level and the addition of AQDS changed the effect of mineral structure property on iron reduction. This result was consistent with the simulation of a reductive dissolution kinetic model, in which the initial iron reduction rate k and long-term Fe(II) ion release extent parameter log γ were controlled by the interaction of the mineral structure property, iron mineral content, and electron shuttle compound distribution. Thus, site-specific environmental conditions should be fully considered in monitoring the performance and environmental effects of biological iron reduction.

10

Improved gene targeting frequency in marine-derived filamentous fungus Aspergillus glaucus by disrupting ligD

54%

Fang Z., Zhang Y., Cai M., Zhang J., Zhang Y., Zhou X.

Journal of Applied Genetics

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2012

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tom 53

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nr 3

Filamentous fungi from the marine environment have shown great potential as cell factories for the production of pharmacologically active metabolites, but extremely low frequency of homologous recombination brings difficulty to further molecular biology studies. To bypass this problem and develop a highly efficient gene targeting system in marine-derived filamentous fungus Aspergillus glaucus, LigD, a homolog of Neurospora crassa Mus-53 which is considered to play a significant role in nonhomologous end joining (NHEJ), was coloned and deleted, and frequency of targeted gene replacement (TGR) increased dramatically from <2 % to 85 % in comparison with that in the wild type, when containing 1000 bp of homologous flanking sequence. Such results strongly indicate that AgLigD is indeed involved in the repair of NHEJ in A. glaucus and functions in this pathway. Furthermore, the AgLigD-defective mutant has no discernible differences with wild type regarding sensitivity to mutagens and UV, growth characteristics and transformation frequency. The AgligD-deficient transformant, as the first NHEJ-defective mutant in the field of marine-derived filamentous fungus, will help in expediting studies of molecular biology of marine-derived microorganisms.

11

Identification of BLAD and citrullinemia carriers in Chinese Holstein cattle

54%

Li J., Wang H., Zhang Y., Hou M., Zhong J., Zhang Y.

Animal Science Papers and Reports

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2011

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tom 29

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nr 1

Bovine leukocyte adhesion deficiency (BLAD) and citrullinemia are two autosomal recessive genetic diseases in Holstein cattle and both result in death of homozygous animals. Through the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technology and DNA sequencing, one citrullinemia and three BLAD carriers were found in a population of 615 Chinese Holstein cattle, including 436 cows and 179 bulls. Citrullinemia and BLAD carrier frequency was 0.16% and 0.49%, respectively, in tested Chinese Holstein cattle. In this study, the first citrullinemia carrier occurring in Chinese Holstein cattle is described, although the world frequency of this disorder is very low.

12

Modulation of virulence genes by the two-component system PhoP - PhoQ in avian pathogenic Escherichia coli

54%

Tu J., Huang B., Zhang Y., Zhang Y., Xue T., Li S., Qi K.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 1

Avian pathogenic Escherichia coli (APEC) infections are a very important problem in the poultry industry. PhoP-PhoQ is a two-component system that regulates virulence genes in APEC. In this study, we constructed strains that lacked the PhoP or PhoQ genes to assess regulation of APEC pathogenicity by the PhoP-PhoQ two-component system. The PhoP mutant strain AE18, PhoQ mutant strain AE19, and PhoP/PhoQ mutant strain AE20 were constructed by the Red homologous recombination method. Swim plates were used to evaluate the motility of the APEC strains, viable bacteria counting was used to assess adhesion and invasion of chick embryo fibroblasts, and Real-Time PCR was used to measure mRNA expression of virulence genes. We first confirmed that AE18, AE19, and AE20 were successfully constructed from the wild-type AE17 strain. AE18, AE19, and AE20 showed significant decreases in motility of 70.97%, 83.87%, and 37.1%, respectively, in comparison with AE17. Moreover, in comparison with AE17, AE18, AE19, and AE20 showed significant decreases of 63.11%, 65.42%, and 30.26%, respectively, in CEF cell adhesion, and significant decreases of 59.83%, 57.82%, and 37.90%, respectively, in CEF cell invasion. In comparison with AE17, transcript levels of sodA, polA, and iss were significantly decreased in AE18, while transcript levels of fimC and iss were significantly decreased in AE19. Our results demonstrate that deletion of PhoP or PhoQ inhibits invasion and adhesion of APEC to CEF cells and significantly reduces APEC virulence by regulating transcription of virulence genes.

13

Downregulation of long non-coding RNA ZXF1 restricts cell survival by targeting miR-634-GRB2 in lung adenocarcinoma

51%

Ren X., Xu N., Zhang Y., Wang T.

Acta Biochimica Polonica

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2020

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tom 67

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nr 1

14

Photosynthetic capacity of the capsule wall and its contribution to carbon fixation and seed yield in castor (Ricinus communis L.)

51%

Zhang Y., Mulpuri S., Liu A.

Acta Physiologiae Plantarum

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2016

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tom 38

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nr 10

15

The effects of temperature on the germination behavior of white, yellow, red and purple maize plant seeds

51%

Deng B., Yang K., Zhang Y., Li Z.

Acta Physiologiae Plantarum

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2015

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tom 37

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nr 08

Even though seeds are hidden inside fruit, they differ in color; an interesting, but puzzling, phenomenon. Here, the antioxidant capacities of white, yellow, red and purple seeds from a single variegated maize plant ear were measured and the effects of temperature (15, 30 and 40 C) on germination behavior assessed. The antioxidant capacity of the seeds followed the order: purple[red[yellow[ white. The most favorable temperature for all of the seeds was 30 C. However, the highest germination rates (GRs) were observed in purple seeds, compared to the other colored seeds, when exposed to 40 C, and white seeds, when exposed to 30 C. The germination speeds among the seeds remained consistent (white[yellow[ red[purple), irrespective of temperature. The impaired germination observed at 40 C was significantly improved by adding exogenous antioxidants, but was further impaired by adding H2O2, especially for the white seeds; similar effects on GR were observed at 30 C and 15 C. High negative (at 30 C) and positive (at 40 C) correlations were observed between antioxidant capacity and GR. However, only high negative correlations were detected between antioxidant capacity and germination speed. In addition, no significant difference in heat stress tolerance was observed in the seedlings produced by the different colored seeds. Thus, we speculate that adverse conditions favor the germination of deeper colored seeds, whereas favorable conditions favor lighter colored ones. We discuss the significance of color, from the perspective of seed antioxidant capacity and germination ability under different environmental conditions.

16

The differentiation of human placenta-derived mesenchymal stem cells into dopaminergic cells in vitro

51%

Chen L., He D.-M., Zhang Y.

Cellular and Molecular Biology Letters

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2009

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tom 14

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nr 3

528-536

Mesenchymal stem cells (MSCs) constitute an interesting cellular source to promote brain regeneration after Parkinson’s disease. MSCs have significant advantages over other stem cell types, and greater potential for immediate clinical application. The aim of this study was to investigate whether MSCs from the human placenta could be induced to differentiate into dopaminergic cells. MSCs from the human placenta were isolated by digestion and density gradient fractionation, and their cell surface glycoproteins were analyzed by flow cytometry. These MSCs were cultured under conditions promoting differetiation into adipocytes and osteoblasts. Using a cocktail that includes basic fibroblast growth factor (bFGF), all trans retinoic acid (RA), ascorbic acid (AA) and 3-isobutyl-1-methylxanthine (IBMX), the MSCs were induced in vitro to become dopamine (DA) neurons. Then, the expression of the mRNA for the Nestin and tyrosine hydroxylase (TH) genes was assayed via RT-PCR. The expression of the Nestin, dopamine transporter (DAT), neuronal nuclear protein (NeuN) and TH proteins was determined via immunofluorescence. The synthesized and secreted DA was determined via ELISA. We found that MSCs from the human placenta exhibited a fibroblastoid morphology. Flow cytometric analyses showed that the MSCs were positive for CD44 and CD29, and negative for CD34, CD45, CD106 and HLA-DR. Moreover, they could be induced into adipocytes and osteocytes. When the MSCs were induced with bFGF, RA, AA and IBMX, they showed a change in morphology to that of neuronal-like cells. The induced cells expressed Nestin and TH mRNA, and the Nestin, DAT, NeuN and TH proteins, and synthesized and secreted DA. Our results suggest that MSCs from the human placenta have the ability to differentiate into dopaminergic cells.

17

Seasonal changes in non-structural carbohydrates and sucrose metabolism enzymes in two Sabina species

51%

Chen T., Pei H., Zhang Y., Qian Q.

Acta Physiologiae Plantarum

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2012

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tom 34

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nr 1

Evergreen plants need to store reserves to allow for their survival during the winter months and for new leaf growth in the following spring. In many of the tree species, these reserve functions are mainly carried out by starch, which is degraded to soluble carbohydrates during the dormant season to maintain active respiration and provide protection against freezing. In the present study, two evergreen woody plants, S. przewalskii Kom. (SP) and S. chinensis (Lin.) Ant.(SC), were used to investigate the patterns of seasonal variation in the concentrations of soluble sugars, sucrose, fructose and starch, and the activities of sucrose-phosphate synthase (SPS), sucrose synthase (SUSY), neutral invertase (NI) and soluble acid invertase (SAI). Foliar soluble sugar, sucrose, fructose, and starch concentrations were markedly higher in SC than in SP; moreover, the activities of SPS, SUSY, NI and SAI were also higher in SC than in SP. There were generally higher concentrations of soluble sugars, sucrose and fructose and SPS activities in the winter than in the summer for both Sabina trees, which was consistent with the necessity for protection against freezing; however, opposite results were found with regards to starch concentrations and the activities of SUSY, NI and SAI. In contrast with the activities of SUSY, NI and SAI, the negative correlation observed between SPS and air temperature was likely a reflection of its crucial role in the acquisition of freezing tolerance by sucrose metabolism in the winter. These results suggest that higher carbon reserves do not give S. chinensis (Lin.) Ant. a competitive advantage in tolerating cold temperatures and that only SPS, but not SUSY, NI and SAI, may play a positive role in freezing tolerance by increasing soluble sugar.

18

Simulation and experimental study of SCR injection system

51%

Du J., Zhang Y., Cai Z.

Polish Maritime Research

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2018

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tom 25

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nr Special Issue S2

According to actual engineering test, we design a linear controlled spray ammonia grille, and apply it to the SCR injection system of ship tail gas treatment. In this paper, the geometric model of ammonia injection grid was built in the flue of diesel engine, and the effects of spray atomization, ammonia uniformity and urea droplet distribution on spray atomization were simulated. Then the test bench was set up to observe the injection status of the ammonia injection grille. The NOx content of the outlet was measured by the original data of the flue gas, and the experimental data were processed. We calculate the denitrification rate by taking the average value to verify the correctness of the spray ammonia grid scheme

19

Glutamate promotes sweet potato storage root swelling by enhancing starch accumulation

51%

Ma H., Li C., Yang S., Zhang Y.

Acta Physiologiae Plantarum

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2020

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tom 42

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nr 04

20

Cloning and expression analysis of Rsk in Brassica napus induced by Sclerotinia sclerotiorum

51%

Zhang Y., Hu C., Zhang C., Gan L.

Acta Physiologiae Plantarum

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2011

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tom 33

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nr 4

Based on the significant differences in the resistance to Sclerotinia sclerotiorum between two Brassica napus cultivars, ZhongR888 and Zhongyou821, near isogenic lines (NILs) were established. The resistance differences between Zhongyou821 and BC₅ progeny (the fifth generation from a cross between Zhongyou821 and ZhongR888 with Zhongyou821 as successive backcross parent) was displayed by cDNA-AFLP and differential transcripts-derived fragments (TDFs). The full-length cDNA of 1,707 bp with 1,323 bp open reading frame (ORF, open box), named Rsk was identified by RT-RACE from the resistance related fragment 40-2 in Zhongyou821. It encoded a protein of 440 amino acid residues, which contained a putative extracellular domain, a transmembrane domain, and an intracellular domain. Analysis of the deduced amino acid sequence of Rsk revealed that it had high homology to Arabidopsis thaliana kinase involved in protein binding, and had a conserved region of LRR-RI, indicating it might be a member of leucine-rich repeats, ribonuclease inhibitor-like subfamily. DNA sequences of 1,685 bp and 1,703 bp without an intron were also identified from Zhongyou821 and BC₅, respectively. Expression analysis showed that Rsk might play a role in disease resistance pathways.

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