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1

Micropropagation of Rehmannia glutinosa Libosch.: production of phenolics and flavonoids and evaluation of antioxidant activity

100%
Piatczak E., Grzegorczyk-Karolak I., Wysokinska H.
Acta Physiologiae Plantarum
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2014
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tom 36
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nr 07
Rehmannia glutinosa Libosch., a valuable medicinal plant, was successfully propagated in vitro using shoot tip explants. Shoot multiplication was performed in glass tubes and in a nutrient sprinkle bioreactor. A mixture of 0.1 mg L⁻¹ indole-3-acetic acid (IAA) and 1.0 mg L⁻¹ of 6-benzylaminopurine in Murashige and Skoog (MS) agar-solidified medium proved the best combination for multiple shoot induction, yielding 8.2 shoots per explant after 4 weeks of culture in glass tubes. The number of shoots increased to 21 per explant when the same combination of growth regulators was used in a nutrient sprinkle bioreactor. The shoots rooted with a frequency of 93 % after 6 weeks of culture on MS agar medium supplemented with IAA (0.1 mg L⁻¹) before being acclimatized in the greenhouse. The antioxidant activities of methanolic extracts from the leaves and roots of the in vitro-regenerated plants of R. glutinosa cultivated in the greenhouse were evaluated using four in vitro assays: scavenging of free radicals (DPPH and ABTS), transition metal reduction and total antioxidant activity phosphomolybdenum test. In all cases, the methanolic extract from leaves demonstrated better antioxidant activity than those taken from roots. A strong correlation was found between total phenolic and flavonoid content, and the antioxidant capacity of the studied extracts.
2

In vitro cultures of Scutellaria alpina as a source of pharmacologically active metabolites

100%
Grzegorczyk-Karolak I., Kuzma L., Wysokinska H.
Acta Physiologiae Plantarum
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2016
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tom 38
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nr 01
3

Study on the chemical composition and antioxidant activity of extracts from shoot culture and regenerated plants of Scutellaria altissima L.

100%
Grzegorczyk-Karolak I., Kuzma L., Wysokinska K.
Acta Physiologiae Plantarum
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2015
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tom 37
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nr 01
The flavonoid (baicalin, wogonoside, luteolin, luteolin-7-glucoside) and verbascoside contents of Scutellaria altissima in both shoot cultures, and the shoots and roots of micropropagated plants grown in the greenhouse for 12 weeks or in the field for 2 years were determined. The level of secondary metabolites was found to be strongly affected by the age and type of plant organ. A comparative analysis of S. altissima plants propagated in vitro and from seeds revealed no differences in the level of secondary metabolites when plants of the same age were studied. The antioxidant potential of methanolic extracts from shoot cultures, and the shoots and roots of S. altissima plants propagated in vitro, were evaluated using ABTS radical scavenging, FRAP metal reduction power and the lipid peroxidation test, in relation to the content of baicalin, wogonoside, verbascoside, total phenolic and total flavonoid compounds. Extracts from the roots of field-grown regenerated plants at the flowering stage were found to possess the strongest antioxidant activity. Correlation analysis revealed that the antioxidant activity of extracts correlated most closely with their total phenolic content estimated by the Folin-Ciocalteu method.
4
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Callus cultures of Harpagophytum procumbens (Burch.) DC. ex Meisn. – production of secondary metabolites and antioxidant activity

100%
Grabkowska R., Matkowski A., Grzegorczyk-Karolak I., Wysokinska H.
BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology
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2015
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tom 96
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nr 1
5

Studies on the antioxidant properties of extracts from the roots and shoots of two Scutellaria species in human blood plasma

100%
Grzegorczyk-Karolak I., Wysokinska H., Olas B.
Acta Biochimica Polonica
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2015
|
tom 62
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nr 2
6

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potentia

88%
Weremczuk-Jezyna I., Grzegorczyk-Karolak I., Frydrych B., Krolicka A., Wysokinska H.
Acta Physiologiae Plantarum
|
2013
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tom 35
|
nr 07
Hairy roots of Dracocephalum moldavica L. were induced using Agrobacterium rhizogenes strain A4. Transformed roots were obtained from shoot explants with low transformation frequency of up to 3 %. The effects of different liquid media: Murashige and Skoog (MS), Gamborg et al. (B5) and Woody Plant (WP) with full- and half-strength (1/2MS, 1/2B5, 1/2WP), on biomass accumulation and rosmarinic acid (RA) content were investigated. The hairy roots were cultured in photoperiod (16 h light/8 h dark) and darkness. Biomass of D. moldavica hairy roots was the highest (7.23 g flask-1 of fresh weight and 0.89 g flask-1 of dry weight) in the cultures grown in WP medium under periodic light. Ultra performance liquid chromatography analysis revealed the highest RA content (78 mg g-1 dry wt) in roots cultured in 1/2B5 medium under photoperiod conditions. It was about tenfold higher compared to roots of field-grown mother plants. Antioxidant activities and total phenolic contents of methanolic extracts of D. moldavica hairy roots cultured in 1/2B5 and WP media under photoperiod and darkness and roots of field grown plants were compared. All extracts were investigated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and phosphomolybdenum reduction assays. Total phenolic contents were estimated by the Folin– Ciocalteu method. The methanolic extract of D. moldavica hairy roots grown in 1/2B5 medium under photoperiod possessed the strongest effects on reducing Mo and DPPH radical scavenging. The activities were significantly higher (p B 0.05) than those of methanolic extract of roots of intact plants grown in the field. The most active methanolic extract of hairy roots was characterized by the highest level of rosmarinic acid and total content of phenolic compounds.
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