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During the growing season, oats is exposed to the pathogenic filamentous fungi, among others of Fusarium spp., which in favorable conditions, may secrete secondary metabolites called mycotoxins that are harmful to the consumer. Grains of five cultivars of naturally contaminated oats were analyzed for Fusarium infection level and mycotoxins (NIV, DON, T-2 and HT-2 toxins) accumulation. The predominant Fusarium species was a common oats pathogen: F. poae (Peck) Wollenw. Among the other identified Fusarium species were F. equiseti (Corda) Sacc. i F. oxysporum Schlecht. emend. Snyd et Hans. The Fusarium fungi infection level of grain was very low for all cultivars and ranged from 2 to 3%. The level of mycotoxins detected in the grain samples was also generally low and ranged from respectively: 53 μg/kg (ppb) for nivalenol, < LOQ for deoxynivalenol and < LOQ for T-2 and HT-2 toxins in the grain of Gniady cv. to respectively: 142 μg/kg (ppb) for nivalenol, < LOQ for deoxynivalenol and 38 μg/kg (ppb) for T-2 and HT-2 toxins in the grain of Krezus cv.
Fungi of the genus Fusarium cause many serious diseases of economic importance. Sensitivity of these species to selected fungicides was evaluated in vitro. Six species of Fusarium and six fungicides: azoxystrobin, prochloraz, thiophanate-methyl, propiconazole, tebuconazole and metconazole were examined. Each species was grown on the PDA (Potato Dextrose Agar) medium supplemented with active substances in concentration of 1, 10 and 100 ppm. The experiment was carried out in four replications. The results of experiment showed a diverse sensibility of Fusarium fungi to fungicides in dependence on their concentrations.
The aim of presented research was an evaluation of fungistatic activity of two strains of Paenibacillus species including preliminary characteristics of metabolites responsible for antagonism. The tested strains inhibited growth of all fungi used as indicators however belonging to Microdochium and Septoria genera were more sensitive than Fusarium. It was observed that larger amount of Paenibacillus sp. added to culture caused usually stronger inhibition of fungal growth and antifungal effect was comparable for both strains. Enzymatic hydrolysis revealed completely lost of antagonistic activity, what indicates that fungistatic metabolites are proteins.
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