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In vitro antibacterial activity of liposomal polymyxin B incorporated into collagen sponge

100%

Trafny E. A., Antos-Bielska M., Grzybowski J.

Cellular and Molecular Biology Letters

1996

tom 01

nr 4

The new antibacterial collagen dressing implant was constructed. The dressing was composed of collagen sponge saturated with liposomal polymyxin B (Lip-PolB). As a control an alternative dressing composed of collagen sponge saturated with free polymyxin B (Free-PolB) was used. The release of the antibiotic from the Lip-PolB was measured by diffusion of the drug into polyurethane sponge saturated with sterile broth employing “polyurethane sponge model” published previously. The antibiotic was released from Lip-PolB four times slower than from Free-PolB. Antibacterial activity of the dressing was tested using also polyurethane sponge model, with polyurethane sponge saturated with the bacterial broth culture of P. aeruginosa (108 CFU/ml). After 1, 2 and 3 days of incubation at 37°C, polyurethane sponges were sonicated in the tubes with PBS; then the CFU number was examined by plating. The Lip-PolB proved again to be several times less effective than Free-PolB. We can draw a conclusion that collagen sponge with liposomal polymyxin B can serve as an effective reservoir of the drug for control of infection in superficial wounds. However, the concentration of liposomal drug applied topically should be several times higher than the concentration of free drug.

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Antimicrobial resistance and genotypes of staphylococci from bovine milk and the cowshed environment

64%

Kot B., Piechota M., Antos-Bielska M., Zdunek E., Wolska K. M., Binek T., Olszewska J., Gulinski P., Trafny E. A.

Polish Journal of Veterinary Sciences

2012

tom 15

nr 4

Investigation of antimicrobial resistance and genetic relatedness of staphylococci from milk of cows with mastitis and cowshed environment was the aim of this study. Antimicrobial resistance against 14 antimicrobials were determined by using a disc diffusion method. Genetic similarity between the most frequently isolated species was analysed by PFGE (pulsed-field gel electrophoresis). Haemolytic activity, DNase, protease and esterase production was also investigated. Coagulase-negative Staphylococcus species were isolated from 30.8% of milk samples from cows with mastitis. The most frequently isolated species was Staphylococcus xylosus and yield of these organisms was significantly associated with milk of mastitis cows. S. epidermidis was a predominant penicillin-resistant species. High frequency of resistance to lincomycin was observed among isolates of S. sciuri (54.2%) and S. xylosus (25.9%) from cows with mastitis. PFGE (pulsed-field gel electrophoresis) analysis of 29 Staphylococcus aureus isolates showed the presence of 17 PFGE pulsotypes. Isolates of S. sciuri (n=36) had unique PFGE patterns. Some S. xylosus isolates from milk and milker’s hands had the same PFGE pulsotypes, and this observation could indicate that dairyman may be a potential source of the infection. The pulsotype of each of the remaining isolates of S. xylosus suggested that they might have come from common environmental sources; however, these isolates differed in antibiotic resistance pattern or virulence traits. Therefore, knowledge about antibiotic sensitivity pattern and virulence factors of a CNS isolate, besides its genotype, may be informative in tracking the source of the infection.

Ograniczanie wyników

1 Cellular and Molecular Biology Letters

1 Polish Journal of Veterinary Sciences

2 Antos-Bielska M.

2 Trafny E. A.

1 Binek T.

1 Grzybowski J.

1 Gulinski P.

1 Kot B.

1 Olszewska J.

1 Piechota M.

1 Wolska K. M.

1 Zdunek E.

1 2012

1 1996

Wyniki wyszukiwania

In vitro antibacterial activity of liposomal polymyxin B incorporated into collagen sponge

Antimicrobial resistance and genotypes of staphylococci from bovine milk and the cowshed environment