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1

Effect of some proteins on biology of grain aphid [Sitobion avenae [F.]]

100%
Sprawka I.
Pestycydy
|
2007
|
nr 3-4
45-51
Extensive studies have been carried out to identify plant proteins with insecticidal properties towards insects. The paper describes a test for screening toxicity and growth inhibition of five commercially available proteins such as: bromelain, honey bee venom, two lectins-wheat germ agglutinin (WGA), concanavalin A (Con A) and lysozyme in artificial diets on Sitobion avenae /F./. Grain aphid proved to be sensitive to all tested proteins. Among the tested proteins concanavalin A and wheat germ agglutinin had the highest negative influence on feeding, reduced weight and increased mortality of tested aphids independently from the dose. It suggested that this group of the proteins have an insecticidal activity and is a good candidate for control of the insect pests.
2
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Toxicity of phytohemagglutinin [PHA] from Phaseolus vulgaris L. to the bird cherry-oat aphid [Rhopalosiphum padi L.]

100%
Sprawka I.
Pestycydy
|
2008
|
nr 3-4
101-108
Recent progress in plant transformation for insect resistance has increased the interest in the potential toxicity of lectins to insect pests. The aim of the present study was to examine the effects of phytohemagglutinin (PHA) on survival, development and fecundity of the bird cherry-oat aphid, when tested on artificial diet. The laboratory tests did not reveal any phagostimulating properties of lectin PHA to R. padi, although the presence of the PHA in the diet decreased fecundity of the adult aphids compared to aphids fed on control diets. Tested lectin had a negative effect on the insects’ weight and induced their mortality. The data presented here suggest that PHA possesses a high insecticidal activity towards the bird cherry-oat aphid and might be considered as protein biopesticide against that aphid pest.
3

Electrophoretic analysis of albumins and globulins isolated from the winter triticale of different resistance to the grain aphid

63%
Ciepiela A. P., Sprawka I.
Cellular and Molecular Biology Letters
|
2001
|
tom 06
|
nr 2
4

The effect of grain aphid [Sitobion avenae F.] feeding on antitrypsin activity in ears of selected spring triticale cultivars

51%
Sprawka I., Ciepiela A. P., Kmoch E.
Pestycydy
|
2005
|
nr 3
189-193
5

Investigation of phenolic acids in leaves of blackcurrant [Ribes nigrum L.] and sour cherry [Prunus cerasus L.]

51%
Chrzanowski G., Sempruch C., Sprawka I.
Electronic Journal of Polish Agricultural Universities. Series Biology
|
2007
|
tom 10
|
nr 4
6

Poziom aminokwasow egzogennych albumin pszenzyta ozimego o roznym stopniu odpornosci na mszyce zbozowa [Sitobion avenae F.]

51%
Ciepiela A. P., Sprawka I., Sempruch C.
Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa
|
2002
|
tom 82
241-244
7

Purification and some physicochemical properties of nitrate reductase isolated from winter triticale seedlings

51%
Sempruch C., Ciepiela A. P., Sprawka I., Chrzanowski G.
Electronic Journal of Polish Agricultural Universities. Series Biology
|
2008
|
tom 11
|
nr 1
8
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Chlorophyll content of aphid-infested seedling leaves of fifteen maize genotypes

51%
Sytykiewicz H., Czerniewicz P., Sprawka I., Krzyzanowski R.
Acta Biologica Cracoviensia. Series Botanica
|
2013
|
tom 55
|
nr 2
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effect of phenolics from woody plants on activity of grain aphid oxidases

45%
Chrzanowski G., Leszczynski B., Sempruch C., Sytykiewicz H., Sprawka I.
Pestycydy
|
2009
|
nr 1-4
63-70
Changes in activity of the grain aphid peroxidase (Px) and polyphenol oxidase (PPO) towards phenolics isolated from leaves of black currant, sour cherry and walnut were examined. Slight increase in activity of peroxidase was found within insect tissues after 24 h of walnut extract treatment, whereas black currant and sour cherry strongly inhibited activity of this enzyme. Later on, the walnut extract reduced activity of the enzyme, finally about 30%. The other extracts showed slight changes in Px activity. The grain aphid’s polyphenol oxidase was stimulated during the first 24 h of the experiment. Further treatment with the phenolics extracts reduced activity of the grain aphid polyphenol oxidase. Generally, phenolics isolated from the black currant and sour cherry were more effective in reducing activity of the aphid peroxidase, whereas phenolics from walnut reduced the polyphenol oxidase activity. Possible application of the phenolics isolated from the woody plants as modern biopesticides towards the grain aphid is discussed.
10

Tolerance of selected winter triticale cultivars to grain aphid

45%
Sprawka I., Ciepiela A. P., Chrzanowski G., Sempruch C., Armacinska J.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
11

Aktywność rybonukleolityczna w liściach czeremchy zwyczajnej w okresie żerowania mszycy czeremchowo-zbożowej (Rhopalosiphum padi L.)

39%
Sytykiewicz H., Czerniewicz P., Leszczynski B., Sempruch C., Golawska S., Sprawka I.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 1
The purpose of performed analyses was to study the impact of Rhopalosiphum padi L. infestation on the level of ribonucleolytic activity in bird cherry leaves during three consecutive vegetation seasons (2003–2005). It was shown that feeding of the bird cherry-oat aphid on P. padus leaves stimulated the activity of acid and alkaline ribonucleases when compared to control (without aphids). Furthermore, the highest rise in activity of both groups of RNases was recorded during increasing and maximal density of aphid population on its primary host shoots.
12

Wpływ zawartości roślinnych związków azotowych na wiosenne migracje mszycy czeremchowo-zbożowej (Rhopalosiphum padi L.)

39%
Czerniewicz P., Sytykiewicz H., Leszczynski B., Chrzanowski G., Sempruch C., Sprawka I.
Progress in Plant Protection
|
2011
|
tom 51
|
nr 1
The results of performed studies showed that migration of Rhopalosiphum padi on secondary hosts in Eastern Poland took place in the second half of May. While the aphid population developed on the primary host a constant decrease in the nitrogen compounds content was observed. During spring migration, individuals of bird cherry-oat aphid colonized secondary hosts that contained a higher amount of total nitrogen and free protein amino acids. Therefore changes in the content of basic nutrients within host plant tissues may be one of the most important factors affecting the host plant alternation by R. padi.
13
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Molecular screening for Bartonella henselae and Borrelia burgdorferi sensu lato co-existence within Ixodes ricinus populations in central and eastern parts of Poland

39%
Sytykiewicz H., Karbowiak G., Werszko J., Czerniewicz P., Sprawka I., Mitrus J.
Annals of Agricultural and Environmental Medicine
|
2012
|
tom 19
|
nr 3
The presented study aimed at establishing the prevalence and co-infection rates of Bartonella henselae and Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from the central and eastern parts of Poland. The common tick individuals were gathered in the years 2008-2009. Questing ticks were sampled by dragging a white woollen flag over lower vegetation at 17 localities within diverse types of habitats: urban recreational green areas (city parks and squares), suburban forests and rural woodlands throughout the investigated regions of Poland. Detection of B. henselae in tested tick specimens was based on PCR amplification of the citrate synthase (gltA) gene, while screening for the presence of B. burgdorferi s.l. DNA was carried out by analyzing fragments of two genes: the flagellin (fla) and outer surface protein A (ospA). A total number of 1,571 I. ricinus ticks were sampled: 865 (55.1%) nymphs, 377 females (24.0%) and 329 males (20.9%). The application of PCR assays revealed that 76 (4.8%) tick samples were B. henselae-positive, B. burgdorferi s.l. DNA was detected in 194 specimens (12.3%), whereas the co-existence of these pathogens was evidenced in 22 tested ticks (1.4%). Furthermore, the occurrence of bartonellae and co-circulation of analysed microorganisms in I. ricinus was affirmed only within adult individuals, while presence of the screened spirochetes was ascertained in both nymphal and adult ticks. It should be stressed that the suburban woods of Warsaw and rural forests in Warsaw County characterized the highest prevalence levels of dual infection with investigated tick-borne pathogens, whereas the lowest co-infection rates were recorded in tick populations inhabiting rural forests in Płock County and forested areas in Korczew-Mogielnica (within the Nadbużański Landscape Park).
14

Biochemical characterisation of chlorophyllase from leaves of selected Prunus species - A comparative study

39%
Sytykiewicz H., Sprawka I., Czerniewicz P., Sempruch C., Leszczynski B., Sikora M.
Acta Biochimica Polonica
|
2013
|
tom 60
|
nr 3
Despite senescence-induced chlorophyll depletion in plants has been widely studied, the enzymatic background of this physiologically regulated process still remains highly unclear. The purpose of this study was to determine selected biochemical properties of partially purified fractions of chlorophyllase (Chlase, chlorophyll chlorophyllido-hydrolase, EC 3.1.1.14) from leaves of three Prunus species: bird cherry (Prunus padus L.), European plum (Prunus domestica L.), and sour cherry (Prunus cerasus L.). Secondarily, this report was aimed at comparing seasonal dynamics of Chlase activity and chlorophyll a (Chl a) content within investigated plant systems. Molecular weight of native Chlase F1 has been estimated at 90 kDa (bird cherry) and approximately 100 kDa (European plum and sour cherry), whereas molecular mass of Chlase F2 varied from 35 kDa (European plum) to 60 kDa (sour cherry). Furthermore, enzyme fractions possessed similar optimal pH values ranging from 7.6 to 8.0. It was found that among a broad panel of tested metal ions, Hg+2, Fe+2, and Cu+2 cations showed the most pronounced inhibitory effect on the activity of Chlase. In contrast, the presence of Mg+2 ions influenced a subtle stimulation of the enzymatic activity. Importantly, although Chlase activity was negatively correlated with the amount of Chl a in leaves of examined Prunus species, detailed comparative analyses revealed an incidental decrement of enzymatic activity in early or moderately senescing leaves. It provides evidence that foliar Chlase is not the only enzyme involved in autumnal chlorophyll breakdown and further in-depth studies elucidating this catabolic process are required.
15

Antifungal activity of Juglans regia (L.) leaf extracts against Candida albicans isolates

39%
Sytykiewicz H., Chrzanowski G., Czerniewicz P., Leszczynski B., Sprawka I., Krzyzanowski R., Matok H.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 3
The objective of performed study was aimed at evaluating the antifungal potential of four extract fractions (methanolic, ethyl acetate, alkaloid, and hydrolyzed methanolic) derived from Juglans regia (L.) leaves against pathogenic Candida albicans strains. Furthermore, total phenolics and alkaloid content as well as the antioxidative potential of examined extract fractions were determined. Tested yeasts comprised 140 isolates from diverse biological specimens (oropharyngeal, rectal and vulvovaginal swabs, skin lesions, sputum, urine, and faeces), and one reference strain (C. albicans ATCC 90029). Methanolic extract from walnut leaves characterized by the highest anticandidal activity, the alkaloid fraction possessed a slightly lower antifungal efficacy, while ethyl acetate and hydrolyzed methanolic preparates inhibited the growth rate of examined fungal pathogens in the lowest degree. Additionally, it has been elucidated that all tested strains were susceptible for nystatin and amphotericin B, and only one yeast strain was resistant to flucytosine. On the contrary, the group of azole antimycotics were characterized by reduced effectiveness against the candidal isolates.
16

Accumulation of gallic and salicylic acid in winter triticale in response to aphid attack

39%
Chrzanowski G., Ciepiela A. P., Sempruch C., Sprawka I., Sytykiewicz H., Czerniewicz P.
Herba Polonica
|
2002
|
tom 48
|
nr 4
Gallic and salicylic acids were extracted with 60% methanol from the ears of two winter triticale cultivars (Dagro and Malno). Separation of phenolic acids was carried out in HPLC column - Eurospher RP-18 with use of KNAUER Maxi-Star K-1000 pump. Mixture of methanol-0.1 M KH₂PO₄ (4.5:5.5) was as mobile phase. Absorbance of analysed phenolic compounds was measured at 230 nm. Obtained results showed that content of gallic and salicylic acids was higher in winter triticale Malno than Dagro. Simultaneously, the increase of salicylic acid concentration was observed in the ears of Malno plants as a result of aphid feeding.
17

Nutritive value of soluble protein of spring triticale ears infested by the grain aphid [Sitobion avenae F.]

39%
Sprawka I., Ciepiela A., Sempruch C., Chrzanowski G., Sytykiewicz H., Czerniewicz P.
Electronic Journal of Polish Agricultural Universities. Series Biology
|
2003
|
tom 06
|
nr 2
The aim of study was to determine the effect of grain aphid (Sitobion avenae /F./) on nutritive value of soluble protein isolated from ears of spring triticale cultivars. Nutritive value of protein was estimated by the limiting amino acid index (CS) calculated in according with to Mitchel and Block (1946), and by the integrated index of essential amino acids (EAA) calculated in with according to Oser (1951). Obtained results showed that feeding of the grain aphid on the ears of tested spring triticale cultivars caused the decrease in the content of essential amino acids. This resulted in the decrease of CS and EAA values and in the reduction of nutritive value of soluble protein.
18

Amino acid composition of albumins and globulins isolated from ears of winter triticale cultivars

39%
Sprawka I., Ciepiela A. P., Sempruch C., Chrzanowski G., Sytykiewicz H., Czerniewicz P.
Herba Polonica
|
2002
|
tom 48
|
nr 4
Determination of soluble protein amino acid content isolated from bird cherry leaves during the spring development (April-May) was done using of ion-exchange chromatography. The fifteen amino acids were identified and determined. It was affirmed that valine was predominant among the all studied amino acids. Obtained results indicate that during spring maturing of bird cherry leaves increases the soluble protein concentration and total amino acid content of analysed protein fraction. It was shown the occurrence statistically significant seasonal changes in the content of ten amino acids: aspartic acid, glutamic acid, lysine, phenylalanine, leucine, isoleucine, tyrosine, methionine, threonine and serine.
19

Amino acid composition of soluble protein in bird cherry [Prunus padus L.] leaves during its spring development

39%
Ciepiela A. P., Sytykiewicz H., Sempruch C., Chrzanowski G., Sprawka I., Czerniewicz P.
Herba Polonica
|
2002
|
tom 48
|
nr 4
Determination the quantitative and qualitative amino acid composition of albumins and globulins isolated from two cultivars of winter triticale was done using of ion-exchange chromatography. The fifteen protein amino acids in the composition of albumins and globulins were identified and determined. The present study proved that the smaller amounts protein amino acids in the fractions albumins and globulins isolated from the Malno cultivar plays an important role in the constitutive resistance to grain aphid.
20

Activity of polyphenoloxidase in the ears of spring wheat and triticale infested by grain aphid [Sitobion avenae F.]

39%
Chrzanowski G., Ciepiela A. P., Sprawka I., Sempruch C., Sytykiewicz H., Czerniewicz P.
Electronic Journal of Polish Agricultural Universities. Series Biology
|
2003
|
tom 06
|
nr 2
Polyphenoloxidase (PPO) plays an important role in plant resistance to insects, and in the detoxication of phenolic compounds taken in the nutrient components. Activity of PPO was examined in the ears of spring wheat and triticale. Extraction of enzymatic protein was carried out from acetone powder with the use of 0.05M phosphoric buffer of pH 7.4. The cultivars of spring wheat have possessed higher activity of PPO than cultivars of triticale. Feeding of the grain aphid has reduced this enzyme activity in the all analysed species and cultivars.
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