Wyniki wyszukiwania

1

Czy rosliny haploidalne przegraly w procesie ewolucji?

100%

Baranski R.

Wszechświat

|

1997

|

tom 98

|

nr 10

251-254

2

In vitro gynogenesis in red beet [Beta vulgaris L]: effects of ovule culture conditions

100%

Baranski R.

Acta Societatis Botanicorum Poloniae

|

1996

|

tom 65

|

nr 1-2

57-60

3

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

Transformacja roślin z wykorzystaniem genu gfp (green fluorescent protein)

100%

Baranski R.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2002

|

tom 488

|

nr 1

Białko zielonej fluorescencji (GFP), dzięki swoim właściwościom fluorescencyjnym, emituje zielone światło i tym samym może być obserwowane w żywych komórkach do których został wprowadzony kodujący je gen gfp. Obecnie wykorzystuje się wiele form białek GFP różniących się od dzikiej formy GFP z Aequorea victoria. Białka te mają zmienione właściwości spektralne, intensywniej fluoryzują i są termostabilne. Wykazano także istnienie innych analogów GFP, które emitują fale z zakresu światła niebieskiego, żółtego i pomarańczowego oraz białek pochodzących z innych organizmów morskich fluoryzujących na czerwono.

4

Ocena wyrownania genetycznego nasion mieszancowych przy wykorzystaniu analizy izoenzymatycznej

100%

Baranski R.

Hodowla Roślin i Nasiennictwo

|

1997

|

nr 4

1-4

5

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

100%

Baranski R.

Acta Physiologiae Plantarum

|

2000

|

tom 22

|

nr 1

6

Indukcja rozwoju niezaplodnionych zalazkow buraka cwiklowego w kulturach in vitro.

88%

Baranski R.

Hodowla Roślin i Nasiennictwo

|

1996

|

nr 2

16-20

7

Linia do obróbki ciasta na chleb do pieca PTC 24/165

88%

Baranski R.

Przegląd Piekarski i Cukierniczy

|

1989

|

nr 3

8

Ocena przydatności markera SCAreoli do selekcji fasoli odpornej na antraknozę

63%

Baranski R., Reby E.

Folia Horticulturae. Suplement

|

2003

|

nr 1

9

A protocol for sonication - assisted Agrobacterium rhizogenesmediated transformation of haploid and diploid sugar beet (Beta vulgaris L.) explants

63%

Klimek-Chodacka M., Baranski R.

Acta Biochimica Polonica

|

2014

|

tom 61

|

nr 1

Hairy root cultures obtained after Agrobacterium rhizogenes-mediated genetic transformation can serve as a model system for studying plant metabolism and physiology, or can be utilized for the production of secondary metabolites. So far no efficient protocol of hairy root development in sugar beet has been publically released. In this work, two A. rhizogenes strains (A4T and LBA1334) carrying a binary vector pBIN-m-gfp5-ER or pCAMBIA1301 possessing gfp and uidA reporter genes were used to transform petiole explants of haploid and diploid sugar beet genotypes. Five treatment combinations of sonicated-assisted Agrobacterium-mediated transformation were compared. Hairy roots appeared on 0% to 54% of explants depending on the treatment combination used. The highest frequency was achieved when explants of a diploid genotype were sonicated for 15 s in the inoculum containing A. rhizogenes of OD600=0.5 and then co-cultured for three days. Using the same treatment combinations the explants of haploid genotypes developed hairy roots with the frequency ranging from 10% to 36%. Transformation efficiency was independent on the bacterial strain used. The results indicate that haploid sugar beet explants are amenable to transformation using A. rhizogenes, and that the efficiency of that process can be increased by applying short ultrasound treatment.

10

Wpływ rożnej zawartości wody na wzrost topoli

63%

Krolikowski L., Baranski R.

Roczniki Gleboznawcze

|

1961

|

tom 10

|

nr Dodatek

11

Badania wzrostu kilku odmian topoli na różnych glebach

63%

Krolikowski L., Baranski R.

Roczniki Gleboznawcze

|

1961

|

tom 10

|

nr Dodatek

12

Intra - population genetic diversity of cultivated carrot (Daucus carota L.) assessed by analysis of microsatellite markers

63%

Maksylewicz A., Baranski R.

Acta Biochimica Polonica

|

2013

|

tom 60

|

nr 4

Intra-population variation of 18 cultivated carrot (Daucus carota L. ssp. sativus) populations of diverse origins was evaluated using codominant microsatellite (SSR) markers. Using 27 genomic and EST-derived SSR markers, 253 alleles were identified with a mean 9.4 alleles per marker. Most of the alleles (60.5%) were rare i.e., with the frequency ≤ 0.05 while only 3.95% of alleles occurred with frequency > 0.6. EST-derived SSR markers were less polymorphic than genomic SSR markers. Differences in allele occurrence allowed 16 out of 18 populations to be assigned to either the Western or Asian carrot gene pools with high probability. Populations could be also discriminated due to the presence of private alleles (25.3% of all alleles). Most populations had excess of alleles in the homozygous state indicating their inbreeding, although heterozygous loci were common in F1 hybrids. Genetic diversity was due to allelic variation among plants within populations (62% of total variation) and between populations (38%). Accessions originating from continental Asia and Europe had more allelic variants and higher diversity than those from Japan and USA. Also, allelic richness and variability in landraces was higher than in F1 hybrids and open-pollinated cultivars

13

Influence of the ploidy level on growth and organogenesis of sugar beet (Beta vulgaris L.) in vitro

63%

Klimek M., Baranski R.

Acta Biologica Cracoviensia. Series Botanica. Supplement

|

2009

|

tom 51

|

nr 1

14

Badania zawartosci cukrow, barwnikow i azotanow w populacjach F2 otrzymanych z krzyzowan miedzy burakiem cwiklowym i cukrowym

63%

Baranski R., Grzebelus D.

Zeszyty Naukowe Akademii Rolniczej w Krakowie. Sesja Naukowa

|

1998

|

tom 57

|

nr 1

49-52

15

Wpływ kwasowości gleby na wzrost topoli Populus hybryd

63%

Krolikowski L., Baranski R.

Roczniki Gleboznawcze

|

1961

|

tom 10

|

nr Dodatek

16

Tissue specific expression of beta-glucuronidase gene driven by heterologous promoters in transgenic cauliflower plants

63%

Baranski R., Puddephat I. J.

Acta Physiologiae Plantarum

|

2004

|

tom 26

|

nr 3

17

Variation of beta-glucuronidase activity in cauliflower plants with gus gene introduced by Agrobacterium rhizogenes mediated transformation

63%

Baranski R., Puddephat I.

Acta Physiologiae Plantarum

|

2003

|

tom 25

|

nr 1

18

Attempts to in planta genetic transformation of carrot

63%

Gladysz K., Baranski R.

Polish Journal of Natural Sciences. Supplement

|

2003

|

tom 01

19

Monitoring the expression of green fluorescent protein in carrot

51%

Baranski R., Klocke E., Ryschka U.

Acta Physiologiae Plantarum

|

2007

|

tom 29

|

nr 3

Green fluorescent protein (GFP) was successfully used as a visual reporter at various stages of carrot (Daucus carota L.) transformation. GFP-fluorescence was non-invasively observed in protoplasts, callus and plants after the delivery of mgfp5-er gene using two transformation methods: direct DNA transfer into polyethylene glycol (PEG) -treated protoplasts and inoculation of root discs with Agrobacterium rhizogenes. Transient GFP-expression was detected in the treated protoplasts and monitored during the first week of the cell culture until the stable level of expression was observed. It was useful for the comparison of protoplast susceptibility to DNA uptake and the transgene expression as the fluorescence declined with various rates depending on the used carrot genotype and PEG-concentration. GFP-monitoring in callus enabled the selection of stably expressing lines. It also allowed verification of the homogeneous tissue composition with regard to the expression of the transgene. In plants, GFP-performance depended on the assayed tissue and organ despite of the constitutive 35S promoter. The expression was visually detected in both vegetative and generative parts, but particularly strong fluorescence was observed in leaf marginal meristems, petioles, stems, and styles. Those tissues can be convenient for examination of the transgenic plants during their growth. The results encourage that GFP is a valuable reporter and can be routinely used for optimization of transformation protocol, selection of transformants and monitoring transgenic carrot.

20

Zawartość karotenów w odmianach marchwi uprawianych na trzech kontynentach

51%

Grzebelus D., Simon P. W., Baranski R., Michalik B.

Folia Horticulturae. Suplement

|

2003

|

nr 1

Ograniczanie wyników

Czy rosliny haploidalne przegraly w procesie ewolucji?

In vitro gynogenesis in red beet [Beta vulgaris L]: effects of ovule culture conditions

Transformacja roślin z wykorzystaniem genu gfp (green fluorescent protein)

Ocena wyrownania genetycznego nasion mieszancowych przy wykorzystaniu analizy izoenzymatycznej

Application of a rapid glucose-6-phosphate isomerase assay in white cabbage breeding

Indukcja rozwoju niezaplodnionych zalazkow buraka cwiklowego w kulturach in vitro.

Linia do obróbki ciasta na chleb do pieca PTC 24/165

Ocena przydatności markera SCAreoli do selekcji fasoli odpornej na antraknozę

A protocol for sonication - assisted Agrobacterium rhizogenesmediated transformation of haploid and diploid sugar beet (Beta vulgaris L.) explants

Wpływ rożnej zawartości wody na wzrost topoli

Badania wzrostu kilku odmian topoli na różnych glebach

Intra - population genetic diversity of cultivated carrot (Daucus carota L.) assessed by analysis of microsatellite markers

Influence of the ploidy level on growth and organogenesis of sugar beet (Beta vulgaris L.) in vitro

Badania zawartosci cukrow, barwnikow i azotanow w populacjach F2 otrzymanych z krzyzowan miedzy burakiem cwiklowym i cukrowym

Wpływ kwasowości gleby na wzrost topoli Populus hybryd

Tissue specific expression of beta-glucuronidase gene driven by heterologous promoters in transgenic cauliflower plants

Variation of beta-glucuronidase activity in cauliflower plants with gus gene introduced by Agrobacterium rhizogenes mediated transformation

Attempts to in planta genetic transformation of carrot

Monitoring the expression of green fluorescent protein in carrot

Zawartość karotenów w odmianach marchwi uprawianych na trzech kontynentach