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1

Bioluminometria - szybka metoda wykrywania zanieczyszczen biologicznych

100%
Bartoszcze M., Bielawska-Drozd A.
Żywienie Człowieka i Metabolizm
|
2007
|
tom 34
|
nr 1-2
530-537
Luminometria ATP, oparta na zjawisku bioluminescencji jest metodą, która obecnie jest w centrum zainteresowania. Jako metoda czuła, szybka i prosta w wykonaniu znajduje zastosowanie w monitorowaniu stanu higieny, jest zalecana do stosowania w systemie HACCP i przydatna do badań żywności, wody, powietrza i powierzchni na obecność skażeń bakteryjnych. Może być efektywnie użyta do wykrywania przetrwalników bakteryjnych jako metoda przesiewowa. Ostatnie badania wskazują, że luminometria może znaleźć także zastosowanie w identyfikacji czynników biologicznych.
2

Zastosowanie metody bioluminometrii do wykrywania przetrwalnikow Bacillus anthracis w wodzie

100%
Bielawska-Drozd A., Bartoszcze M.
Żywienie Człowieka i Metabolizm
|
2007
|
tom 34
|
nr 1-2
524-529
Przeprowadzono badania nad zastosowaniem bioluminometrii do szybkiego wykrywania obecności przetrwalników Bacillus anthracis w zawiesinie i w wodzie. Czułość metody bioluminometrycznej wynosiła 29.700 CFU/ml. Statystycznie znamienne wyniki w porównaniu z kontrolą otrzymano przy stężeniu przetrwalników Bacillus anthracis 10⁴ CFU/ml po 10 minutach inkubacji.
3

Wystepowanie przetrwalnikow Bacillus anthracis w srodowisku

100%
Bielawska-Drozd A., Bartoszcze M.
Medycyna Weterynaryjna
|
2007
|
tom 63
|
nr 08
946-950
93 environmental samples were collected and tested using molecular biology techniques. The presence of chromosomal sequence Ba813 was detected in three soil samples (SS1, SS3, ZL2) and in three dental pulp samples (ZZ3, ZZ5, ZZ8). The presence of pag gene (210 bp) was observed in SS3 and ZL2 samples. There were no positive reactions with primers targeting the capsule encoding gene (777 bp). Multiplex PCR reaction enables identification of capC gene (264 bp) in five samples: SS3, ZL2, ZZ3, ZZ5, ZZ8. The presence of pag gene (SS3, ZL2) and cap gene (SS3, ZL2, ZZ5) were observed in standard PCR. Bacillus anthracis spores were detected by SMART test in three samples: SS3, ZL2 and ZZ5. These strains were non-hemolytic, sensitive to penicillin and gamma phage. The PCR positive for pag gene isolates were able to produce PA (protective antigen).
4

Impact of climate change on infectious diseases

81%
Mirski T., Bartoszcze M., Bielawska-Drozd A.
Polish Journal of Environmental Studies
|
2012
|
tom 21
|
nr 3
Global climate change is a common phenomenon today. It is mainly caused by increasing greenhouse gas emissions. It has been proven that global climate change affect the emergence and spread of infectious diseases. This applies to both climate change as a whole, as well as individual factors such as temperature, rainfall, humidity, etc. These changes may directly impact the pathogen, and indirectly the vectors of these pathogens. They can also affect the resistance of humans and animals. The association between the emergence of infectious disease outbreaks and global climate change was also shown. This problem should be taken seriously when considering the development of effective prevention programs.
5

Nowe możliwości hamowania infekcji B. anthracis

81%
Zakowska D., Bartoszcze M., Niemcewicz M., Bielawska-Drozd A.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 10
To date, antibiotics have been the primary drugs used in the treatment of anthrax infections. However, their effectiveness is questionable, especially during the phase of intensive toxin production in the course of infection, and the number of drug-resistant strains continues to rise. Successful treatment of anthrax infection is therefore becoming more difficult. The article discusses some of the new methods of inhibiting anthrax infections: the inhibition of spore germination and of the attachment of PA to the host cell receptor, the inhibition of the enzymatic process of cleaving PA into PA63 and PA20, and of PA63 oligomerization, endocytosis and translocation (their influence on the protection of macrophages against lysis was also discussed). In addition, the neutralization of B. anthracis LeTx and EdTx toxins was presented as another potential method of inhibiting anthrax infections.
6

The evaluation of methods for detection of Bacillus anthracis spores in artificially contaminated soil samples

81%
Bielawska-Drozd A., Niemcewicz M., Bartoszcze M.
Polish Journal of Environmental Studies
|
2008
|
tom 17
|
nr 1
5-10
The nested PCR has been used to evaluate the usefulness and efficiency of different Bacillus anthracis spore isolation methods in contaminated soil samples. The best results were obtained using two methods described by Beyer et al. [1] and Cheun et al. [9]. Outer and inner pairs of primers were designed from the protective antigen gene of plasmid pXO1 as well as from genes B and C of the capsule region of the plasmid pXO2. The influence of soil types on obtained results was also studied. The type of soil samples did not affect the nested PCR results. Furthermore, the sensitivity of nested PCR and PCR – ELISA was also examined.
7
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Review of studies on SARS-CoV-2 infection inhibitors

81%
Michalski A., Wojcicka P., Bielawska-Drozd A., Bartoszcze M.
Annals of Agricultural and Environmental Medicine
|
2021
|
tom 28
|
nr 4
8
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Lessons learned from 2001-2021 - from the bioterrorism to the pandemic era

81%
Michalski A., Knap J., Bielawska-Drozd A., Bartoszcze M.
Annals of Agricultural and Environmental Medicine
|
2022
|
tom 29
|
nr 1
9

Brucella - virulence factors, pathogenesis and treatment

71%
Glowacka P., Zakowska D., Naylor K., Niemcewicz M., Bielawska-Drozd A.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 2
Brucellae are Gram-negative, small rods infecting mammals and capable of causing disease called brucellosis. The infection results in abortion and sterility in domestic animals (sheeps, pigs, rams etc). Especially dangerous for humans are: Brucella melitensis, Brucella suis, Brucella abortus, and Brucella canis that trigger unspecific symptoms (flu-like manifestation). Brucella rods are introduced via host cells, by inhalation, skin abrasions, ingestion or mucosal membranes. The most important feature of Brucella is the ability to survive and multiply within both phagocytic and non-phagocytic cells. Brucella does not produce classical virulence factors: exotoxin, cytolisins, exoenzymes, plasmids, fimbria, and drug resistant forms. Major virulence factors are: lipopolysaccharide (LPS), T4SS secretion system and BvrR/BvrS system, which allow interaction with host cell surface, formation of an early, late BCV (Brucella Containing Vacuole) and interaction with endoplasmic reticulum (ER) when the bacteria multiply. The treatment of brucellosis is based on two-drug therapy, the most common combinations of antibiotics are: doxycycline with rifampicin or fluoroquinolones with rifampicin. Currently, also other methods are used to disrupt Brucella intracellular replication (tauroursodeoxycholic acid or ginseng saponin fraction A).
10
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New aspects of the infection mechanisms of B. anthracis

71%
Zakowska D., Bartoszcze M., Niemcewicz M., Bielawska-Drozd A., Kocik J.
Annals of Agricultural and Environmental Medicine
|
2012
|
tom 19
|
nr 4
11

Peptydy przeciwdrobnoustrojowe - nowe możliwości zwalczania infekcji u ludzi i zwierząt

71%
Mirski T., Gryko R., Bartoszcze M., Bielawska-Drozd A., Tyszkiewicz W.
Medycyna Weterynaryjna
|
2011
|
tom 67
|
nr 08
Antimicrobial peptides (AMPs), also called peptide antibiotics, have been discovered in the early 1980s in frogs They were antimicrobial substances called magainins. AMPs are among the oldest defense mechanisms in plants, humans and animals. The major peptides include i.a. defensins, cathelicidins and protegrins. The mechanisms of action of antimicrobial peptides rely on the permeabilization of the microbial membrane, destabilization of the lipid bilayer structure, creation of micelles or channels within the membrane, binding lipopolysaccharide (LPS), preventing DNA replication, inhibiting protein expression, releasing ATP, as well as binding free iron and removing it from the microbial growth environment. At present, intensive research is being conducted on the use of AMPs in human and veterinary medicine, including treatment of infections such as acne, skin infections, sepsis, and bacterial infections of the diabetic foot. Among others, the following preparations are being tested: Ambicin, for the treatment of infections caused by Mycobacterium, and Iseganan, protegrin for the treatment of mouth inflammation, CF and chronic lung infections. P. aeruginosa-infected animals treated with the D2A21 preparation showed 100% survival. Some of the AMPs show biocidal activity against Bacillus anthracis. Defensins isolated from the mucus and tissues of many fish species have the ability to protect fish from infections by Aeromonas hydrophila, Pseudomonas fluorescens, and Vibrio anguillarum. Beneficial effects of using defensins in the treatment of Borrelia burgdorferi infections in dogs have been described. Synthetic peptides are used for the production of a vaccine against parvovirosis. Peptides obtained from lactic acid bacteria (LAB) reduce the contamination and increase the stability of food products. AMPs are also useful for decontaminating the environment and medical equipment, as well as for sterilizing catheters. They have also been used to develop biocidal self-disinfecting surfaces (BSOs). Moreover, AMPs can be used in hospital hygiene and veterinary medicine, e.g., for the treatment of protective clothing, wipes, filters, ventilation, etc.
12

Detection of Coxiella burnetii and Francisella tularensis in tissues of wild-living animals and in ticks of north-west Poland

61%
Bielawska-Drozd A., Cieslik P., Zakowska D., Glowacka P., Wlizlo-Skowronek B., Zieba P., Zdun A.
Polish Journal of Microbiology
|
2018
|
tom 67
|
nr 4
This work presents results of the research on the occurrence of Coxiella burnetii and Francisella tularensis in the tissues of wild-living animals and ticks collected from Drawsko County, West Pomeranian Voivodeship. The real-time PCR testing for the pathogens comprised 928 samples of animal internal organs and 1551 ticks. The presence of C. burnetii was detected in 3% of wild-living animals and in 0.45–3.45% (dependent on collection areas) of ticks. The genetic sequences of F. tularensis were present in 0.49 % of ticks (only in one location – Drawa) and were not detected in animal tissues. The results indicate respectively low proportion of animals and ticks infected with C. burnetii and F. tularensis.
13

Identyfikacja Francisella tularensis techniką real - time PCR z wykorzystaniem sond hybrydyzujących zaprojektowanych dla fragmentów sekwencji genów fopA i tul4

61%
Bielawska-Drozd A., Niemcewicz M., Gawel J., Bartoszcze M., Graniak G., Joniec J., Kolodziej M.
Medycyna Doświadczalna i Mikrobiologia
|
2010
|
tom 62
|
nr 4
Badano przydatność zaprojektowanych starterów FopA F/R, Tul4 F/R i sond hybrydyzujących FopA S1/S2, Tul4 S1/S2 dla genów fopA i tul4 do wykrywania F. tularensis. W badaniach, w których użyto 50 szczepów F. tularensis uzyskano wyniki dodatnie. Swoistość reakcji wykazano badając szczepy bakterii non-Francisella tularensis. Przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 zaprojektowanych dla genów fopA dodatnie wyniki amplifikacji uzyskano ze wszystkimi badanymi szczepami F. tularensis. Identyczne wyniki otrzymano w reakcji real - time PCR z zastosowaniem starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 zaprojektowanych dla genu tul4. Swoiste produkty reakcji amplifikacji pojawiły się między 16 a 18 cyklem reakcji. Badania z użyciem starterów i sond hybrydyzujących zaprojektowanych dla genu fopA wykazały, że charakterystyczna temperatura topnienia produktów wynosiła 61°C, a dla genu tul4 60°C. Przy użyciu starterów Tul4 F/R i sond hybrydyzujących Tul4 S1/ S2 czułość oznaczeń wynosiła 10 fg/µl, a przy użyciu starterów FopA F/R i sond hybrydyzujących FopA S1/S2 1 fg/µl.
14
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Q fever - selected issues

61%
Bielawska-Drozd A., Cieslik P., Mirski T., Bartoszcze M., Knap J. P., Gawel J., Zakowska D.
Annals of Agricultural and Environmental Medicine
|
2013
|
tom 20
|
nr 2
Q fever is an infectious disease of humans and animals caused by Gram-negative coccobacillus Coxiella burnetii, belonging to the Legionellales order, Coxiellaceae family. The presented study compares selected features of the bacteria genome, including chromosome and plasmids QpH1, QpRS, QpDG and QpDV. The pathomechanism of infection – starting from internalization of the bacteria to its release from infected cell are thoroughly described. The drugs of choice for the treatment of acute Q fever are tetracyclines, macrolides and quinolones. Some other antimicrobials are also active against C. burnetii, namely, telitromycines and tigecyclines (glicylcycline). Q-VAX vaccine induces strong and long-term immunity in humans. Coxevac vaccine for goat and sheep can reduce the number of infections and abortions, as well as decrease the environmental transmission of the pathogen. Using the microarrays technique, about 50 proteins has been identified which could be used in the future for the production of vaccine against Q fever. The routine method of C. burnetii culture is proliferation within cell lines; however, an artificial culture medium has recently been developed. The growth of bacteria in a reduced oxygen (2.5%) atmosphere was obtained after just 6 days. In serology, using the IF method as positive titers, the IgM antibody level >1:64 and IgG antibody level >1:256 (against II phase antigens) has been considered. In molecular diagnostics of C. burnetii infection, the most frequently used method is PCR and its modifications; namely, nested PCR and real time PCR which detect target sequences, such as htpAB and IS1111, chromosome genes (com1), genes specific for different types of plasmids and transposase genes. Although Q fever was diagnosed in Poland in 1956, the data about the occurrence of the disease are incomplete. Comprehensive studies on the current status of Q fever in Poland, with special focus on pathogen reservoirs and vectors, the sources of infection and molecular characteristics of bacteria should be conducted.
15
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Microarrays - new possibilities for detecting biological factors hazardous for humans and animals, and for use in environmental protection

61%
Mirski T., Bartoszcze M., Bielawska-Drozd A., Gryko R., Kocik J., Niemcewicz M., Chomiczewski K.
Annals of Agricultural and Environmental Medicine
|
2016
|
tom 23
|
nr 1
16

Development of real-time PCR assay for detection of vibrio cholerae

61%
Bielawska-Drozd A., Mirski T., Bartoszcze M., Cieslik P., Roszkowiak A., Michalski A.
Polish Journal of Environmental Studies
|
2012
|
tom 21
|
nr 2
Vibrio cholerae is the etiological agent of epidemic cholera. Symptoms include stomach ache, bloody diarrhea, and vomiting, which lead to severe dehydration and even death. Environmental monitoring as well as rapid and accurate identification of this pathogen are important for public health protection. In this study, a real-time PCR method for the detection of toxigenic V. cholerae was developed. In total, 63 environmental and clinical strains were tested for the presence of seven targets, namely ompW, ompU, tcpA, ctxA, zot, rfbO1, and rfbO139. The proposed method is specific, simple, and fast, and can be used for detection of toxigenic and non-toxigenic V. cholerae strains. The minimum detection limit of this assay for V. cholerae in environmental water samples was 1.4 CFU/ml.
17

Detection of enterohaemorrhagic Escherichia coli by triplex real time PCR method

61%
Bielawska-Drozd A., Cieslik P., Bartoszcze M., Mirski T., Osek J., Gryko R.
Bulletin of the Veterinary Institute in Puławy
|
2011
|
tom 55
|
nr 4
Triplex real time PCR method for detection of enterohaemorrhagic strains of Escherichia coli in meat samples was described. Total of 36 strains belonging to different serogroups were used. All strains were cultured in brain heart infusion broth and sorbitol MacConkey agar plates under aerobic conditions. Sets of primers and TaqMan probes were prepared using GenBank resources. Using the designed method we were able to detect bacteria in meat samples and determine genotypes for stx1, stx2, and eaeA genes in tested strains, simultaneously. The described method provides a rapid detection of single colonies of this pathogen in minced meat samples.
18

Microbial purity assessment of herbal raw materials by bioluminometric method

61%
Mietkiewicz-Capala A., Malm A., Bartnik M., Glowniak K., Michalski A., Bielawska-Drozd A., Bartoszcze M.
Herba Polonica
|
2004
|
tom 50
|
nr 3-4
The paper presents the application of the bioluminometric method to assess microbial purity of some herbal raw materials, such as: Lavendae Flos, Hippocastani Flos, Crategi Inflorescentia, Altheae Folium, Betulae Folium, Salviae Folium, Solidaginis Herbae, Chelidonii Herbae and Taraxaci Herbae. The method is based on determination of ATP level originating from bacterial cells. All the raw-materials were examined with FP VI method in order to compare these methods.
19

Genetic diversity among Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis strains using repetitive element polymorphism-PCR

61%
Brumlik M. J., Bielawska-Drozd A., Zakowska D., Liang X., Spalletta R. A., Patra G., Delvecchio V. G.
Polish Journal of Microbiology
|
2004
|
tom 53
|
nr 4
Repetitive element polymorphism-PCR (REP-PCR) is one of the tools that has been used to elucidate genetic diversity of related microorganisms. Using the MB1 primer, REP-PCR fingerprints from 110 Bacillus strains within the "B.cereus group" have identified eighteen distinct categories, while other more distantly related bacterial species fell within six additional categories. All Bacillus anthracis strains tested were found to be monomorphic by fluorophore-enhanced REP-PCR (FERP) fingerprinting using the MB1 primer. In contrast, other non- B.anthracis isolates displayed a high degree of polymorphism. Dendrogramic analysis revealed that the non- B.anthracis strains possessing the Ba813 chromosomal marker were divided into two clusters. One of the clusters shared identity with the B.cereus strains examined.
20
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Bacillus anthracis infections – new possibilities of treatment

52%
Zakowska D., Bartoszcze M., Niemcewicz M., Bielawska-Drozd A., Knap J., Cieslik P., Chomiczewski K., Kocik J.
Annals of Agricultural and Environmental Medicine
|
2015
|
tom 22
|
nr 2
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