Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik

The effect of ethyl methane sulphonate and diethyl sulphate on chilli (Capsicum annuum L.) in M1 generation

100%

Sanjai Gandhi E., Sri Devi A., Mullainathan L.

International Letters of Natural Sciences

2014

tom 05

In recent years, the demand of chilli has tremendously increased due to its attractive market price and multifarious used in cooked and processed forms. At present people are much concerned about the fruit quality and yield. Therefore, attention is being paid for development of genotypes having high yield potential with desirable fruit quality characters in a short period of time. For this purpose, seeds of chilli were mutagenised with ethyl methane sulphonate (EMS) and diethyl sulphate (DES) to determine their mutagenic sensitivity in M1 generation. The increasing concentration of EMS and DES decreased in morphological and yield characters. The spectrum of mutation and induced variability for various quantitative traits were observed in M1 generation such as germination (%), plant height, primary and secondary branches per plant, days to first flowering, fruit length (cm), fruit girth (cm), total number of fruits per plant, number of seeds per fruit, seed weight per fruit (g), 100 seed weight (g) and pericarp: seed ratio showed variability in chilli with the effect of EMS and DES. The percentage of chromosomal abnormalities in different mitotic stages was significantly higher than that of the control in all the treatment concentrations.

Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker

100%

Mullainathan L., Sridevi A., Umavathi S., Sanjai Gandhi E.

International Letters of Natural Sciences

2014

tom 06

The present study was under taken in order to analyze the chemical mutagenesis on Chilli germplasm. In this regard, K1 variety of chilli was subjected to different mutagenic concentration for inducing mutagenesis. The M3 plants exposed to EMS and DES to produce clear difference from the untreated control, thus indicating that mutagenic treatment produce polymorphic regions in the chilli. For extraction of genomic DNA was adopted an improved protocol of CTAB method with slight modification. A total of ten primers were used to screen the polymorphism among the treated populations line tall, tall with chlorophyll deficient, leaf, flower, GMS and DNA damages in maturity mutants were analyzed with control. Out of ten primers, four primers (PGF02, PGF03, PGF04 AND OP107) were successfully amplified in all the samples used for this study. The successful primers were amplified in to 93 products showing an average of 9.3 bands.

Ograniczanie wyników

2 International Letters of Natural Sciences

2 Mullainathan L.

2 Sanjai Gandhi E.

1 Sri Devi A.

1 Sridevi A.

1 Umavathi S.

2 2014

Wyniki wyszukiwania

The effect of ethyl methane sulphonate and diethyl sulphate on chilli (Capsicum annuum L.) in M1 generation

Genetic variation in mutants of chilli (Capsicum annuum) revealed by RAPD marker