Three hybridoma cell lines (AC2, DC6, PC2) secreting monoclonal antibodies (MCA) against protein 27, a major component of group specific antigens of avian leucosis viruses, have been employed. All three monoclonal antibodies reacted with p27. The epitope topological studies demonstrated that MCA, DC6 and PC2 were directed to different epitopes of ALV-p27. Comparative studies of different MCA sets for the detection of ALV gs-antigens with the ELISA test demonstrated that the sensitivity of the test, using MCA PC2 for adsorption and a HRP as a conjugate, was the same as one in the commercial kit and was higher when compared with another commercial DAS ELISA kit.
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