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1

Evaluation of meat waste composting process based on fecal streptococci survival

100%
Ligocka A., Paluszak Z.
Polish Journal of Environmental Studies
|
2008
|
tom 17
|
nr 5
739-744
Animal by-products of category 3 after proper processing may be a valuable fertilizer for agricultural purposes. However, they can contain numerous bacterial and viral pathogens and, in cases of improper handling, may pose a health risk for people and animals. This study is aimed at monitoring the number of fecal streptococci introduced into carriers of different types imitating fragments of meat and bone wastes during composting process in a drum bio-reactor. Fecal streptococci are indicator microorganisms, and are known by their thermoresistant characteristics, so it was assumed that their elimination will also diminish the pathogenic microorganisms present in wastes. Three research cycles were carried out in a drum-type bio-reactor, and a different course of temperature was noted in each of them. In cycle 1, in which the temperature exceeded 60ºC, fecal streptococci died the fastest, 139.0-154.4 hours later (depending on carrier type). In cycles 2 and 3, maximum temperatures were similar (57.2ºC and 58.8ºC, respectively), but secondary multiplication of the streptococci in the 102nd hour of the processes was observed. In cycle 2 at this time their number was similar to the level of initial suspension. The type and size of the carriers were of no major importance to streptococci survival in the bio-reactor. Yet in each of the cycles analyzed, effective reduction was accomplished and the product obtained can be considered to be environmentally safe.
2

Effectiveness of different sanitisation technologies on the inactivation of Ascaris suum eggs in organic waste

100%
Ligocka A., Paluszak Z.
Bulletin of the Veterinary Institute in Puławy
|
2009
|
tom 53
|
nr 4
The aim of this study was to examine the rate of Ascaris suum egg inactivation in meat wastes subjected to different sanitisation technologies. The wastes were composted in a drum bioreactor and fermented under mesophilic (37°C) and thermophilic (55°C) conditions. A suspension with fertilised eggs of A. suum was introduced into perlon sacks and placed in waste biomass. They were removed at fixed-time intervals and the percentage of live eggs was calculated. The eggs were inactivated the earliest during thermophilic fermentation and their total time of survival amounted to approximately 10 h. The full inactivation of eggs during composting was obtained after 72 h, while in the course of mesophilic fermentation. No live eggs were detected on 14th d. All the sanitisation technologies analysed ensured the environmental safety of product obtained.
3

Badania mikrobiologiczne gleby aluwialnej skazonej gnojowica

100%
Paluszak Z., Ligocka A.
Roczniki Gleboznawcze
|
2003
|
tom 54
|
nr 1-2
117-124
4

Rola receptorow aktywowanych przez proliferatory peroksysomow gamma [PPARgamma] w procesie adipogenezy oraz rozwoju otylosci i cukrzycy typu 2

100%
Bogacka I., Ligocka A.
Postępy Biologii Komórki
|
2008
|
tom 35
|
nr 1
15-30
5
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Characteristics of the macro – and microelements in benthic sediments of selected mid-field ponds

100%
Brysiewicz A., Ligocka A.
Infrastruktura i Ekologia Terenów Wiejskich
|
2018
|
nr IV/1
6

Capability of lactic acid bacteria to inhibit pathogens in sewage sludge subjected to biotechnological processes

100%
Ligocka A., Paluszak Z.
Bulletin of the Veterinary Institute in Puławy
|
2005
|
tom 49
|
nr 1
7

Validation of meat waste composting process based on Escherichia coli survival rate

100%
Ligocka A., Paluszak Z.
Bulletin of the Veterinary Institute in Puławy
|
2008
|
tom 52
|
nr 1
Escherichia coli suspension was introduced into meat and bone carriers of different sizes and a number of the bacteria was analysed at suitable time intervals. The cycles analysed were characterised by a slightly different distribution of temperature, but it was sufficient to inactivate the rods in all types of the carriers. The quickest elimination rate of E. coli (0.1-0.14 log/h) was noted in the cycle in which the temperature ranged from 50 to 60°C. In the other cycles, with lower temperatures generated, the decrease in the bacteria's numbers was 0.03-0.04 log/h. However, the inactivation of the introduced pathogens occurred in both cycles. When properly conducted, a process of composting wastes guarantees retaining environmental safety.
8

Antagonism of bacteria from Lactobacillus genus towards bacterial and fungal phytopathogens

100%
Ligocka A., Paluszak Z.
Phytopathologia Polonica
|
2003
|
tom 28
9
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Wpływ systemu uprawy i herbicydu Topogard 50 WP na skład mykoflory zasiedlającej ryzoplanę korzeni grochu siewnego odmiany Agat

81%
Ligocka A., Paluszak Z., Sadowski S., Dziedzic T.
Acta Scientiarum Polonorum. Agricultura
|
2005
|
tom 04
|
nr 1
Groch siewny uprawiany w monokulturze cechował wyższy stopień porażenia korzeni w porównaniu ze zmianowaniem, głównie za sprawą grzybów Fusarium oxysporum i F. solani. W okresie kwitnienia porażenie roślin występowało silniej niż w okresie wschodów. Najbardziej wrażliwe na działanie herbicydu Topogard okazały się grzyby: F. oxysporum, Trichoderma viride, Phoma glomerata i Cephalosporium asperum – dawka mniejsza od polowej istotnie hamowała wzrost grzybni. Najmniej wrażliwe na herbicyd były grzyby F. avenaceum, Rhizoctonia solani oraz Zygorhynchus spp.
10

Inaktywacja jaj Ascaris suum w kompostowanych osadach posciekowych

81%
Paluszak Z., Ligocka A., Olszewska H.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 02
154-156
11

Effects of green algae and napa cabbage on life-history parameters and gut microflora of Archegozetes longisetosus (Acari: Oribatida) under laboratory conditions

81%
Seniczak A., Ligocka A., Seniczak S., Paluszak Z.
Biological Letters
|
2016
|
tom 53
|
nr 2
12
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Survival rate of Suid Herpesvirus (SuHV-1, Aujeszky`s disease virus, ADV) in composted sewage sludge

81%
Paluszak Z., Lipowski A., Ligocka A.
Polish Journal of Veterinary Sciences
|
2012
|
tom 15
|
nr 1
Sewage sludge constitutes a source of valuable biogenic raw materials, but it is a carrier of many pathogenic microorganisms and viruses. Subjected to an effective sanitization by means of the process of composting, it is suitable to use in agriculture as fertilizers. The aim of this study was to observe the survival rate of Suid Herpesvirus under the influence of the temperature alone (water bath) as well as in sewage sludge subjected to the process of composting (pile). The samples were taken at different time intervals, and the virus titres were determined. The viruses survived considerably longer under laboratory conditions: at 30°C as long as 21 days, at 40°C - 93 hours, and at 50°C - less than an hour. In the compost pile, in spite of the lack of the thermophylic phase, the total survival time of the viruses ranged from 34 to 44.5 hours, which indicates the vast importance of other physicochemical factors, apart from the temperature, contributing to virus inactivation.
13

Effects of green algae and napa cabbage on life-history parameters and gut microflora of Archegozetes longisetosus (Acari: Oribatida) under laboratory conditions

81%
Seniczak A., Ligocka A., Seniczak S., Paluszak Z.
Biological Letters
|
2016
|
tom 53
|
nr 1
14

Wplyw czynnikow fizykochemicznych na skutecznosc dzialania bakteriocyn bakterii mlekowych na patogeny osadow posciekowych

81%
Ligocka A., Paluszak Z., Hermann J.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 12
1413-1416
15
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Survival of BPV and Aujeszky's disease viruses in meat wastes subjected to different sanitization processes

81%
Paluszak Z., Lipowski A., Ligocka A.
Polish Journal of Veterinary Sciences
|
2010
|
tom 13
|
nr 4
The effect of composting and anaerobic fermentations under meso- and thermophylic conditions (37° and 55℃) on the survival of bovine parvovirus (BPV) and Aujeszky’s disease viruse (ADV) in meat wastes has been examined in this study. Viruses were adsorbed on filters and introduced into carriers which were made of meat fragments of different sizes and bones or in the form of suspension they were introduced into the biomass in the course of processes of waste treatment. Carriers were removed at appropriate time intervals and virus titres were determined. The thermoresistant parvovirus survived for the longest time during mesophylic fermentation (almost 70 days), slightly shorter during composting (7-9.5 days depending on the type of carrier) and for the shortest time – at 55℃ (46-76 hours). Its inactivation rate was the fastest in a suspension, slower in meat and bone carriers. ADV inactivation proceeded considerably faster, as compared with BPV. Its active particles were not detected as early as in the 30th minute of thermophylic fermentation, the 6th hour of mesophylic fermentation and at the first sampling time during composting (at the 72nd hour). Total survival time ranged from 50 min to 13 hours. All the tested technologies enabled the effective elimination of ADV and on average twofold decrease in BPV titre. From the study conducted it follows that of both viruses, the BPV should be applied for validation processes of methods used in meat waste processing, particularly if this refers to methods where higher temperature is the factor inactivating pathogens.
16

The influence on nonthermal, low-pressure plasma on microbiological contamination of food

81%
Ligocka A., Skowron K., Rytlewski P., Bauza-Kaszewska J.
Polish Journal of Natural Sciences
|
2018
|
tom 33
|
nr 4
Among many methods for food decontamination, high hopes are associated with nonthermal, low-pressure plasma technology. This study aimed to estimate the survival rate of Escherichia coli, Salmonella Senftenberg W775 and Enterococcus faecalis exposed to the action of plasma for the time from 0 to 800 s. As carriers there were used the laurel leaves. An analogous experiment was performed with the use of UV-C radiation. The results show that the plasma technology is more effective in bacteria reduction than ultraviolet radiation. The cells of E. coli, were the most susceptible to the action of nonthermal plasma and their numbers after 800s decreased by 7 log, after the same time the amount of enterococci decreased by about 6 log. Salmonella cells were characterized by a very high resistance to low-pressure plasma. Results of this study show that the plasma technology should be used for a longer time and in a higher dose to guarantee the full decontamination of food
17
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Enzymatyczne i antagonistyczne właściwości fluoryzujących pałeczek z rodzaju Pseudomonas, wyizolowanych z ryzoplany lnu włóknistego

81%
Ligocka A., Bauza-Kaszewska J., Paluszak Z.
Acta Scientiarum Polonorum. Agricultura
|
2005
|
tom 04
|
nr 2
47-55
W pracy zbadano stopień aktywności enzymów pektynolitycznych, celulolitycznych, amylolitycznych i proteolitycznych, a także zdolność do uruchamiania nierozpuszczalnych fosforanów przez fluoryzujące pałeczki z rodzaju Pseudomonas. Bakterie wyizolowano z ryzoplany lnu włóknistego, uprawianego w systemie zmianowania i monokultury. Żaden z izolatów nie wykazywał aktywności enzymatycznej wobec celulozy, natomiast pozostałe wielocukry hydrolizowane były przez wszystkie izolaty. Bakterie były najbardziej aktywne wobec białka. Oceniono również ich właściwości antagonistyczne względem wybranych patogenów grzybowych wyizolowanych z tego samego środowiska. Wszystkie z nich, chociaż w różnym stopniu, hamowały rozwój grzybów.
18

Wpływ temperatury na tempo inaktywacji EHEC w kompostowanych osadach pościekowych

81%
Paluszak Z., Bauza-Kaszewska J., Ligocka A.
Prace Wydziału Nauk Przyrodniczych. Seria B. Prace Komisji Nauk Rolniczych i Biologicznych
|
2004
|
tom 39[52]
19
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Effects of spring season solar drying process on sanitation indicators in sewage sludge and potential as a method for fertilizer production

81%
Sypula M., Paluszak Z., Ligocka A., Skowron K.
Annals of Agricultural and Environmental Medicine
|
2013
|
tom 20
|
nr 1
The agricultural use of sewage sludge is possible on condition of maintaining microbiological and parasitological standards, and one of the most modern methods improving its sanitary state is solar drying. In the presented study, the effect of this process on the elimination of indicator microorganisms (Escherichia coli, Salmonella Senftenberg W775, Enterococcus spp.) and eggs of Ascaris suum introduced into the biomass of sludge was examined. The experiment was carried out in the spring period with a maximal temperature of 18 °C inside the drying plant. Bacteria and parasite eggs were introduced into special carriers (cylinders filled with sewage sludge) and placed at selected points of the drier. The carriers were removed every 7 days and subject to a research procedure in order to estimate the number of bacteria and percentage of live eggs of Ascaris suum. Sanitization of the material was not obtained, since after 28 days of the process the final product contained a large concentration of Enterococcus spp. and S. Senftenberg W775 (105-109 MPNg-1). Only the number of E. coli decreased by 6 log. During the process, the fastest decrease in the number of bacteria was observed in E. coli (ca 0.2 log/day), slower in enterococci (0.02-0.081 log/day), and the slowest in bacilli of the genus Salmonella (0.011-0.061 log/day). Sludge after drying also still contained 57-66% of live eggs of A. suum. The study proved that the solar drying of sludge in the spring period results in a product which poses a hazard for public and animal health and environmental sustainability, and should not be used as a fertilizer.
20

Przezywalnosc paleczek Salmonella senftenberg W775 w osadach posciekowych poddanych procesowi kompostowania

81%
Paluszak Z., Bauza-Kaszewska J., Ligocka A.
Medycyna Weterynaryjna
|
2003
|
tom 59
|
nr 03
239-242
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