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1

Porównanie metod immunoenzymatycznych stosowanych w monitorowaniu stężenia tacrolimusu we krwi pacjentów po transplantacji narządów

100%
Bartlomiejczyk I., Zochowska D., Matuszewicz D., Paczek L.
Diagnostyka Laboratoryjna
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2007
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tom 43
|
nr 3
2

HPLC vs metoda immunochemiczna - wyniki badania korelacji metod oznaczania sirolimusa

100%
Zochowska D., Bartlomiejczyk I., Kaminska A., Paczek L.
Diagnostyka Laboratoryjna
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2007
|
tom 43
|
nr 3
3
Content available remote

The serum levels of growth factors: PDGF, TGF-beta and VEGF are increased after strenuous physical exercise

100%
Czarkowska-Paczek B., Bartlomiejczyk I., Przybylski J.
Journal of Physiology and Pharmacology
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2006
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tom 57
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nr 2
Strenuous physical exercise induces muscle fibers damage and non-specific inflammatory response. Activated by inflammatory process cells may serve as the source of wide spectrum of inflammatory mediators and growth factors. Namely Platelet Derived Growth Factor (PDGF), Transforming Growth Factor-ß (TGF-ß) and Vascular Endothelial Growth Factor (VEGF) could be released. The aim of present study was to assess the impact of physical exercise on growth factors generation in healthy young people. 14 young sportsmen were enrolled into the study. They performed strenuous physical exercise. Blood samples were drawn before, immediately after, and 2 hours after the exercise bout. Serum PDGF, TGF-beta and VEGF concentrations were measured using commercially available ELISA kit based on immunoenzimatic method. Serum level of PDGF increased significantly from 1.7 ng /ml before to 4.64 ng /ml (2.73-fold) immediately after, and to 3.3 ng /ml (1.94-fold) 2 hours after exertion. Serum level of TGF-beta increased significantly from 20.58 ng /ml before to 55.37 ng /ml (2.7-fold) immediately after, and to 40.03 ng /ml (1.95-fold) 2 hours after exertion. Serum level of VEGF increased significantly from 91.83 pg /ml before to 165.61 pg /ml (1.8-fold) immediately after the exercise. Two hours after the exertion serum level of VEGF was 137.22 pg /ml, what is 1.49-fold above the basal level; however not being significantly different. In summery, observed increased level of growth factors could be involved in the process of adaptation of human organism to physical training. In addition, in the context of the role of inflammation in the pathogenesis of various diseases, our results point to the potentially deleterious effect of strenuous physical exercise.
4
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The effect of acute and prolonged endurance exercise on transforming growth factor-ß1 generation in rat skeletal and heart muscle

88%
Czarkowska-Paczek B., Zendzian-Piotrowska M., Bartlomiejczyk I., Przybylski J., Gorski J.
Journal of Physiology and Pharmacology
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2009
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tom 60
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nr 4
157-162
The serum level of the transforming growth factor-beta1 (TGF-ß1) is elevated after acute bouts of exercise and prolonged training, as well as after myocardial infarction. However, the source of this increase remains unclear. Contracting skeletal muscles are known to be the source of many cytokines. To determine whether skeletal or heart muscles produce TGF-ß1 during exercise, we investigated the effect of a single bout of acute exercise on TGF-ß1 generation in skeletal and heart muscles in untrained rats (UT, n=30) and in rats subjected to prolonged (6-week) endurance training (T, n=29). The UT and T (a day after final training) groups were subjected to an acute bout of exercise with the same work load. Rats from both groups were sacrificed and skeletal and heart muscle samples were collected before (pre), immediately after (0 h), or 3 hours (3 h) after acute exercise. TGF-ß1 mRNA was quantified by RT-PCR in these samples, and basal TGF-ß1 protein levels were determined in skeletal muscle in the UTpre and Tpre subgroups by ELISA. Acute exercise caused a non-significant increase in TGF-ß1 mRNA in skeletal muscle in UT0h rats, in compare to UTpre rats. There was a significant decrease of TGF-ß1 mRNA in the T0h group (p=0.0013) in compare to Tpre rats. Prolonged training caused a significant increase in TGF-ß1 mRNA (p=0.02); however, the TGF-ß1 protein level decreased (p=0.02). In heart muscle, there was a significant decrease of TGF-ß1 mRNA in UT0h (p=0.01) and UT3h (p=0.04) compared to UTpre rats. TGF-ß1 mRNA levels were unchanged in T0h and T3h compared to Tpre; basal TGF-ß1 mRNA expression after training was also unchanged (UTpre vs. Tpre). We conclude that physical exercise is a potent stimulus for inducing TGF-ß1 gene expression in skeletal muscle, but does not increase the protein level. Thus, skeletal and heart muscle do not contribute to increased serum levels of TGF-ß1 after physical exercise.
5

Poziom cytokin u pacjentów przewlekle dializowanych

88%
Bartlomiejczyk I., Gomolka M., Gerwatowska D., Niemczyk S., Paczek L.
Diagnostyka Laboratoryjna
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2004
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tom 40
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nr 3
6

Porównanie metod immunologicznych EMIT i CMIA - oznaczanie stężenia tacrolimusu we krwi pacjentów po przeszczepieniu nerki lub wątroby. Doniesienie wstępne

76%
Bartlomiejczyk I., Wirkowska A., Wyczalkowska-Tomasik A., Garlinska M., Gala K., Paczek L.
Diagnostyka Laboratoryjna
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2010
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tom 46
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nr 4
7

Involvement of beta2-microglobulin in CD69 expression on T cells

76%
Paczek L., Czarkowska-Paczek B., Korczak-Kowalska G., Wierzbicki P., Bartlomiejczyk I., Gorski A.
Archivum Immunologiae et Therapiae Experimentalis
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2001
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tom 49
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nr 3
239-242
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