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1

Isolation and characterization of heavy metal - resistant bacterias capable of removing Cr(VI)

100%
Wang P., Ma Y., Wang C., Zhang S., Cheng S.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 1
A total of 53 strains of chromium-resistant bacteria were isolated from Meiliang Bay of Taihu Lake, China and were tested for Cr(VI) resistance. The strain THKCS311 with the maximum growth value under Cr(VI) stress was regarded as the optimal strain for further study. The partial sequences were amplified from the strain and the BLAST query revealed that it was closely related to Bacillus sp., and it had 98% homologous to Bacillus mycoides strain 273 and Bacillus anthracis strain ATCC 14578. Batch experiments were conducted to remove Cr(VI) using THKCS311, and the effects of the initial Cr(VI) concentration, pH, and temperature condition on Cr(VI) removal efficiency were investigated. The results showed that Bacillus sp. can mediate reduction of Cr(VI)-Cr(III), and the removal efficiency decreased with the increase of initial Cr(VI) concentration. The removal efficiency of Cr(VI) was highest at pH 6.5 and 35ºC, and removal efficiencies were 59.2% and 60.7%, respectively. SEM micrographs indicated that THKCS311 cells were irregular and cracked with the appearance of wrinkles on the surface after Cr(VI) stress.
2

RhTSG-6 inhibits IL-1beta-induced extracellular matrix degradation and apoptosis by suppressing the p38, and JNK pathways in nucleus pulposus cells

86%
Pei S., Ying J., Zhand Y., Su L., Cheng S., Ruan D.
Folia Histochemica et Cytobiologica
|
2020
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tom 58
|
nr 3
3

Dynamic risk assessment method of tunnel collapse based on attribute interval assessment model and application

86%
Wang S., Li L., Cheng S., Hu H., jin H., Gao S.
Polish Journal of Environmental Studies
|
2020
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tom 29
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nr 5
4

Mechanisms of of vascular endothelial growth factor synthesis by hydrogen peroxide and overexpression of copper-zinc superoxide dismutase: role of heme oxygenase-1

86%
Cisowski J., Grzenkowicz-Wydra J., Cheng S., Agarwal A., Jozkowicz A., Podhajska A., Dulak J.
Acta Biochimica Polonica
|
2003
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tom 50
|
nr Supplement 1
5

Composition of ethyl acetate extracts from three plant materials (shaddock peel, pomegranate peel, pomegranate seed) and their algicidal activities

86%
Wang H., Kang H., Zhang L., Cheng S., Liu H., Liu H., Sun B.
Polish Journal of Environmental Studies
|
2015
|
tom 24
|
nr 4
Many studies have involved the isolation and identification of allelochemicals from aquatic plants, but the algicidal properties of terrestrial plants have received less attention. This study aims to identify allelochemicals of ethyl acetate extracts from three plant materials (shaddock peel, pomegranate peel, pomegranate seed) and to investigate their inhibitory effects on Microcystis aeruginosa. The ethyl acetate extracts of the three plant materials were identified by GC-MS. Finally, 19 kinds of compounds (including organic acids, ester, ketone, sterol, etc.) were obtained and eight kinds of organic acids and N-phenyl-2-Naphthalenamine were proved to be allelochemicals. The inhibitory effects of the ethyl acetate extracts were also explored by M. aeruginosa bioassay. This showed that the inhibition percentages of ethyl acetate extracts of the three plant materials on the growth of M. aeruginosa were 43.9%, 47.5%, and 40.3%, respectively, when the algae were treated at a dosage of 20 mg/L extracts.
6

Salinity improves chilling resistance in Suaeda salsa

86%
Cheng S., Yang Z., Wang M., Song J., Sui N., Fan H.
Acta Physiologiae Plantarum
|
2014
|
tom 36
|
nr 07
Suaeda salsa L., a C3 euhalophytic herb, is native to saline soils, demonstrates high resistance to salinity stress. The effect of chilling stress on S. salsa under high salinity, particularly the change in unsaturated fatty acid content within membrane lipids, has not been investigated. After a 12 h chilling treatment (4°C) performed under low irradiance (100 μmol m⁻² s⁻¹), the chlorophyll contents, maximal photochemical efficiency of photosystem II (Fv/Fm) and actual PSII efficiency (ΦPSII) were determined. These measurements were significantly decreased in S. salsa leaves in the absence of salt treatment yet there were no significant changes with a 200 mM NaCl treatment. Chlorophyll contents, Fv/Fm and ΦPSII in S. salsa under 200 mM NaCl were higher than those without salt treatment. The unsaturated fatty acid content and the double bond index (DBI) of major membrane lipids of monogalactosyldiacylglycerols, digalactosyldiacylglycerols (DGDG), sulphoquinovosyldiacylglycerols and phosphatidylglycerols (PG) significantly increased following the chilling treatment (4°C) (with 12 h of low irradiance and 200 mM of NaCl). The DBI of DGDG and PG was decreased in the absence of the salt treatment. These results suggest that in the euhalophyte S. salsa, a 200 mM NaCl treatment increases chilling tolerance under conditions of low irradiance (100 μmol m⁻² s⁻¹).
7

Characterization and expression patterns of a cinnamate-4-hydroxylase gene involved in lignin biosynthesis and in response to various stresses and hormonal treatments in Ginkgo biloba

86%
Cheng S., Yan J., Meng X., Zhang W., Liao Y., Ye J., Xu F.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 01
Plant cell walls primarily comprise lignin, which performs functions of mechanical support, water transport, and stress responses. Lignin biosynthesis pathway proceeds through metabolic grid featuring complexity and diversity in enzymatic reaction. Cinnamate-4-hydroxylase (C4H, EC 1.14.13.11) is the gene encoding enzyme that catalyzes the second step of phenylpropanoid pathway responsible for biosynthesis of lignin. A full-length cDNA of C4H (designated as GbC4H), which spanned 1816-bp with a 1518-bp open reading frame encoding a 505-amino-acid protein, was cloned from Ginkgo biloba. A GbC4H genomic DNA fragment, spanning 3249-bp, was cloned and found to contain two exons and one intron. GbC4H protein showed high similarities with other plant C4Hs to include conserved domains of cytochrome P450 family. GT-1, W-box, and Myb/Myc recognition sites involved in stress response were detected in a 1265-bp upstream promoter region of GbC4H. Phylogenetic analysis suggested the common evolutionary ancestor shared by plant C4Hs including the gymnosperm enzyme. pET-28a-GbC4H plasmid was constructed and expressed in Escherichia coli strain BL21. Enzymatic assay revealed that recombinant GbC4H protein catalyzes conversion of trans-cinnamic acid to p-coumaric acid. Expression analyses in different organs showed high expression of GbC4H in stems and roots, whereas low expressions was found in fruits, carpopodium, and petioles. Further analysis indicated linear correlation of lignin contents with transcript levels of GbC4H among different tissues. GbC4H transcription was increased by treatments with UV-B, cold, salicylic acid, and abscisic acid, indicating the possible role of GbC4H in response to stresses and hormonal signal. Understanding of GbC4H function could benefit molecular breeding and reinforcement of defense mechanisms in Ginkgo.
8

Molecular survey and genetic diversity of Babesia spp. and Theileria spp. in Cattle in Gansu province, China

72%
Sun M., Guan G., Liu Z., Wang J., Wang D., Wang S., Ma C., Cheng S., Yin H., Luo J.
Acta Parasitologica
|
2020
|
tom 65
|
nr 2
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