Preferencje help
Polish version English version Język
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 22

Liczba wyników na stronie
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników

Wyniki wyszukiwania

help Sortuj według:

help Ogranicz wyniki do:
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
1

Occurance of poly (ADP-ribose) polymerase in Phaseolus coccineus (L.) plants exposed to cadmium

100%
Patrzylas P., Pawlikowska-Pawlega B., Jakubowicz-Gil J., Skorzynska-Polit E.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2009
|
tom 51
|
nr 2
2

The influence of apigenin on membrane and action potential in the liverwort Conocephalum conicum

100%
Pawlikowska-Pawlega B., Krol E., Trebacz K., Gawron A.
Acta Physiologiae Plantarum
|
2007
|
tom 29
|
nr 2
Apigenin (4',5,7-trixydroxyflavone) is a member of the family of plant flavonoids considered to prevent a number of human diseases, for instance cancer development. It displays a lot of activities and part of its beneficial effects could come from its affinity to the cellular membranes. In the present study we used the liverwort Conocephalum conicum, a model plant in electrophysiological study. Intracellular microelectrode measurements were carried out to examine the effects of apigenin alone and in combination with verapamil on the resting and action potentials. The application of apigenin caused an increase of action potential amplitudes. An increase even by 110–131% with respect to the control was observed. Little increase was also found in the membrane potentials in apigenin treated plants. Verapamil, the known calcium channel inhibitor, caused gradual decline of AP amplitudes. When apigenin was used simultaneously with verapamil, still high APs were observed. Duration of action potentials amplitudes measuerd at a half of the amplitude decreased in either apigenin or apigenin and verapamil treated plants to 56–62% of the control. It is concluded that apigenin strongly affects the membranes and prevents inhibitory effect of verapamil probably interacting with calcium channel protein.
3

Protective effect of quercetin and apigenin on hydrogen peroxideinduced apoptosis in HeLa cells

100%
Pawlikowska-Pawlega B., Jarosz-Wilkolazka A., Paduch R., Zarzyka B.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
4

Quercetin and genistein hindering effect of neomycin action in the liverwort Conocephalum conicum

100%
Pawlikowska-Pawlega B., Krol E., Trebacz K., Gawron A.
Acta Physiologiae Plantarum
|
2011
|
tom 33
|
nr 4
Apigenin, quercetin and genistein are members of the family of plant flavonoids suspected to prevent a number of human diseases, for instance cancer development. They display a number of activities, and part of their beneficial effects may be due to their affinity to cellular membranes. In this study, we used Conocephalum conicum, a well-elaborated model of liverworts. Intracellular microelectrode measurements were carried out to examine the effects of flavonoids in combination with neomycin on the resting and action potentials. Neomycin triggered gradual decline of action potential amplitudes through a membrane potential decrease (membrane potential became less negative) and a decrease of the action potential peak value. Additionally, duration of action potential amplitudes measured at half of the amplitude increased in neomycintreated plants. However, the simultaneous use of quercetin or genistein (but not apigenin) with neomycin hindered neomycin-specific actions. Hence, the membrane resting potential and action potential amplitudes regained neomycin-free values. It may be concluded that application of at least some flavonoids (namely quercetin and genistein) exerts strong influence on electrical membrane responses in C. conicum.
5

Effects of quercetin and verapamil on membrane potential in the liverwort Conocephalum conicum

100%
Pawlikowska-Pawlega B., Trebacz K., Krol E., Gawron A.
Acta Physiologiae Plantarum
|
2000
|
tom 22
|
nr 1
6

Effects of apigenin on membrane potential in the liverwort Conocephalum conicum

100%
Pawlikowska-Pawlega B., Krol E., Trebacz K., Gawron A.
Biological Letters
|
2005
|
tom 42
|
nr 2 Spec.Vol.
7

The effect of quercetin on apoptosis and necrosis induction in human colon adenocarcinoma cell line LS180

100%
Pawlikowska-Pawlega B., Jakubowicz-Gil J., Rzymowska J., Gawron A.
Folia Histochemica et Cytobiologica
|
2001
|
tom 39
|
nr 2
8

Corneal endothelial cell cultures

100%
Rzymowska J., Gawron A., Swiech-Zubilewicz A., Pawlikowska-Pawlega B.
Folia Histochemica et Cytobiologica. Supplement
|
1997
|
tom 35
|
nr 2
9

Investigations of hippocampal astrocytes in lipopolysaccharide-preconditioned rats in the pilocarpine model of epilepsy

88%
Jaworska-Adamu J., Dmowska M., Cybulska R., Krawczyk A., Pawlikowska-Pawlega B.
Folia Histochemica et Cytobiologica
|
2011
|
tom 49
|
nr 2
10

Immunolocalization of lipoxygenase in the anther of Gagea lutea (L.) Ker.-Gaw.

88%
Szczuka E., Skorzynska-Polit E., Pawlikowska-Pawlega B., Sobieska J., Gawron A.
Acta Biologica Cracoviensia. Series Botanica. Supplement
|
2005
|
tom 47
|
nr 1
11

Wplyw furanokumaryn peucedaniny i bergaptenu, izolowanych z owocow Peucedanum tauricum Bieb. [Apiaceae] na indukcje apoptozy i ekspresje bialek szoku termicznego w komorkach HeLa

88%
Bartnik M., Glowniak K., Jakubowicz-Gil J., Pawlikowska-Pawlega B., Gawron A.
Herba Polonica
|
2006
|
tom 52
|
nr 3
58-59
12

Characterisation of a novel bioflocculan tproduced by Rhodococcus opacus strain

88%
Czernierska M., Kapral J., Szczes A., Pawlikowska-Pawlega B., Jarosz-Wilkolazka A.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
13

Immunolocalization of lipoxygenase in the anther of Gagea lutea [L.] Ker.-Gaw.

88%
Szczuka E., Skorzynska-Polit E., Pawlikowska-Pawlega B., Sobieska J., Gawron A.
Acta Biologica Cracoviensia. Series Botanica
|
2006
|
tom 48
|
nr 1
Immunogold labelling revealed the presence of lipoxygenase (LOX) in different parts and types of anther cells of Gagea lutea. LOX was found in the cytoplasm and close to ER elements in epidermal and endothecial cells, and close to the cell walls of the latter. The positive immunoreaction to LOX was less intense in the middle layers and the loculus of the anther, where single immunogold particles were concentrated at the cell walls of these layers and in the protoplast masses, in vacuoles, close to mitochondria, inside plastids, and in the liquid of the anther cavity. LOX occurred in the cytoplasm and around ER elements of pollen grains as well as in the exine layer, particularly in contact regions between the outer and inner exine layers. The correlations between LOX localization in different anther cells and the functioning of particular anther parts are discussed.
14
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
Content available

Localization and activity of lipoxygenase in Cd-treated seedlings of Phaseolus coccineus

88%
Skorzynska-Polit E., Pawlikowska-Pawlega B., Szczuka E., Plak A., Melke J.
Acta Societatis Botanicorum Poloniae
|
2005
|
tom 74
|
nr 3
Lipoxygenase was localized in the primary leaves of Phaseolus coccineus (L.), seedlings treated with 25 µM Cd and in control plants using the immunogold method. The enzyme was localized mainly in the peripheral parts of protoplast of control plant cells. It was found in the cell wall, along the ER elements, at plastid lamellae and inside the mitochondria. In Cd-treated seedlings the elements of parenchyma cells showed an atypical inner structure. The immunolabelling of LOX was less intensive in comparison with control. The enzyme was found in the cytoplasm, at the cell wall area, vacuoles and in the plastid stroma as single gold particles. LOX activity optima were determined at pH 7.0 and 8.0 for both linoleic and linolenic acid used as substrates. After 2 days of seedlings exposure to Cd the activity of LOX decreased at pH 7.0 and 8.0 when linoleic acid was used as substrate, and strongly declined at pH 7.0 after 4 days of the metal treatment. When linolenic acid was the substrate LOX activity slightly increased after 2 days of the plants exposure to Cd, but after 4 days it rapidly decreased at pH 7.0. The changes in LOX activity are discussed.
15

Quercetin suppresses heat shock-induced nuclear translocation of Hsp72

88%
Jakubowicz-Gil J., Pawlikowska-Pawlega B., Piersiak T., Pawelec J., Gawron A.
Folia Histochemica et Cytobiologica
|
2005
|
tom 43
|
nr 3
16

Modifying effect of quercetin on the lipid membrane

88%
Wojtowicz K., Pawlikowska-Pawlega B., Gawron A., Misiak L. E., Gruszecki W. I.
Folia Histochemica et Cytobiologica. Supplement
|
1996
|
tom 34
|
nr 1
17

The effect of quercetin on induction of apoptosis

88%
Rzymowska J., Gawron A., Pawlikowska-Pawlega B., Jakubowicz-Gil J., Wojcierowski J.
Folia Histochemica et Cytobiologica
|
1999
|
tom 37
|
nr 2
18

Effect of peucedanin and bergapten (5-MOP), furanocoumarins isolated from Peucedanum tauricum Bieb. (Apiaceae) fruits, on apoptosis induction and heat-shock protein expression in HeLa cells

88%
Bartnik M., Glowniak K., Jakubowicz-Gil J., Pawlikowska-Pawlega B., Gawron A.
Herba Polonica
|
2006
|
tom 52
|
nr 4
In the research effects of bergapten (5-MOP) and peucedanin, furanocoumarins isolated from fruits of Peucedanum tauricum Bieb. (Apiaceae), on apoptosis, necrosis and heat-shock proteins Hsp 27 and Hsp 72 expression in human carcinoma cells HeLa B (ECACC No 85060701) were preliminary studied. The purity of analysed compounds was confirmed by RP HPLC, EI-MS and 1D, 2D NMR. For apoptosis and necrosis detection the mixture of Hoechst 33342 and propidium iodide (PI) was used. Early apoptosis was detected using Annexin V Test. Cells were incubated with furanocoumarins at the concentrations of 1, 5, 10 and 15 μg/ml. The level of heat-shock protein expression was determined by Western blot technique. Quantitative analysis of Hsp 27 and Hsp 72 was done with the Bio-Profil Bio-1D Windows Application V.99.03 program. The obtained results were analysed for significance by one-way ANOVA test. As a result of our experiments we found that peucedanin was more effective in apoptosis induction than bergapten. In HeLa cells treated with peucedanin only low necrosis was observed. The incubation with 15 μg/ml of peucedanin for 24 hours inhibited Hsp 72 expression in cells by 77.5% (± 0.05), and Hsp 27 by 74.0% (± 0.02) after five hours of treatment as compared to the control. The inhibition of Hsps expression after peucedanin treatment may be responsible for increased apoptosis induction (11 .4%) detected by Annexine V test. Incubation with furanocoumarins has an influence on morphology of HeLa cells (many cytoplasmic protrudings and bulges of “blebbing” cytoplasm were observed).
19

Changes of the cell wall structure as a part of the response system of white rot fungus: Abortiporus biennis to oxidative stress

76%
Jaszek M., Pawlikowska-Pawlega B., Laniak J., Wazna J., Zawadzki P., Grzywnowicz K.
Acta Biochimica Polonica
|
2007
|
tom 54
|
nr Supplement 4
20

Cytotoxic potential analysis of biomaterials modified with proteolytic enzyme inhibitors

76%
Szalapata K., Piet M., Kapral J., Paduch R., Pawlikowska-Pawlega B., Osinska-Jaroszuk M., Jarosz-Wilkolazka A.
Acta Biochimica Polonica
|
2018
|
tom 65
|
nr S2
Pierwsza strona wyników Pięć stron wyników wstecz Poprzednia strona wyników Strona / 2 Następna strona wyników Pięć stron wyników wprzód Ostatnia strona wyników
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.