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1

The comparative aspects of the overlap between limbic and somato-motor zones in the claustrum

100%
Majak K., Kowianski P., Karwacki Z., Morys J.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
2

General anaesthesia in rats undergoing experiments on the central nervous systems

100%
Karwacki Z., Kowianski P., Morys J.
Folia Morphologica
|
2001
|
tom 60
|
nr 4
235-242
The rat is one of the species most commonly used in laboratory practice. Numerous publications concerning various aspects of morphology and physiology are based on the results obtained in this species. It make these results comparable and under some precautions enables to transpose into the relationships observed in humans. Each experimental project must obtain the permission of the Local Ethical Committee, as well as comply with the regulations of the European Communities Council, outlined in the “European Convention for the protection of vertebrate animals used for experimental and other scientific purposes”. Adequate pre-operative care can eliminate or reduce the incidence of many complications, which may occur during anaesthesia. General anaesthesia in experimental practice can be achieved using a variety of drugs and ways of administration, among others inhalational or intravenous. The side effects of anaesthetic agents can be reduced in this way. Knowledge of the effect of anaesthetics on the cerebral circulation, metabolism and intracranial pressure in both normal and pathological conditions is crucial for neurobiological purposes. Many anaesthetic agents depress respiration, which can result in hypoxia, hypercapnia and acidosis. To maintain blood carbon dioxide and oxygen concentration in the physiological range, it is necessary to apply tracheal intubation and artificial ventilation. However, even when using sophisticated equipment, the role of basic clinical observation, such as the colour of the blood shed in the operation field, breathing depth and frequency, cannot be overestimated. The importance of monitoring mean arterial blood pressure and intracranial pressure in experiments on the central nervous system is fundamental. Special attention should be paid to controlling the temperature and monitoring the fluid balance. Appropriate postoperative care can have a decisive influence on the final results of the research.
3

The different patterns of maturation of the claustrocortical connections in a rabbit

100%
Kowianski P., Dziewiatkowski J., Karwacki Z., Morys J.
Folia Morphologica
|
2000
|
tom 59
|
nr 3
The quantitative analysis of the claustrocortical connections labeled with the fluorescent retrograde tracer Fluoro-Gold (FG) was conducted on 90 rabbits subdivided into the following age groups (P2, P7, P14, P21, P30, P60, P90, P120, P180). The equal volumes of retrograde fluorescent tracer FluoroGold (FG) were injected into the selected regions of the motor or somatosensory cortices. The volume of the dorsal part of the claustrum, total number of projecting neurons, numerical density and percentage distribution of projecting neurons were estimated by means of the unbiased stereological methods. The claustrocortical connections both with the motor and somatosensory areas in a rabbit are established in the postnatal life. The parts of the claustrum occupied by the motor and somatosensory projection zones as well as the morphology of the cortically projecting neurons do not reveal characteristic changes during the studied period. The significant decrease of the total number and numerical density of cortically projecting neurons as well as the increase of the claustral volume may reflect the process of adjustment of the claustrum to its modulatory function upon corresponding cortical areas. The intensity of the claustral connections with the motor and somatosensory cortices reveals significant difference during the studied period, being higher for the motor projection. It may be assumed that the claustrocortical connections established before birth undergo significant quantitative changes during postnatal development.
4

Does the sevorane influence the intracranial pressure (ICP) in the rabbits with experimental intracerebral hemorrhage ?

88%
Karwacki Z., Kowianski P., Dziewiatkowski J., Morys J., Suchorzewska J.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
5

The technique of inhalation anaesthesia in experimental investigation in the rat

88%
Karwacki Z., Kowianski P., Witkowska M., Karwacka M., Morys J.
Folia Morphologica
|
2006
|
tom 65
|
nr 4
414-418
The rat is the most frequently used animal in scientific inquiry conducted for the purpose of advancing basic knowledge that may lead to an improvement in the results of treatment. Understanding of the pharmacological properties of inhalation anaesthetics, in combination with monitoring of their concentration in the inspired and end-tidal gas, together provide safe and precise control of the depth of the anaesthesia. However, accurate application of the inhalation method of anaesthesia requires special equipment for the delivery and effective scavenging of inhalation anaesthetics.
6

Influeuce of the experimental intraventricular hemorrhage on the intracranial pressure and hemodynamic changes in the rabbit

88%
Karwacki Z., Kowianski P., Dziewiatkowski J., Morys J., Suchorzewska J.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
7

Descending connections of the claustrocortical neuronal loop in the rat and rabbit

88%
Kowianski P., Morys J., Dziewiatkowski J., Karwacki Z., Spodnik J.
Acta Neurobiologiae Experimentalis
|
1997
|
tom 57
|
nr 5
8

The effect of sevoflurane on the intracranial pressure (ICP) and cardiovascular system in the rabbit

76%
Karwacki Z., Kowianski P., Dziewiatkowski J., Tobiasz E., Morys J., Suchorzewska J.
Acta Neurobiologiae Experimentalis
|
1999
|
tom 59
|
nr 3
9

Time-dependent changes in microglia reaction in the course of experimental intracerebral haematoma in the rat

76%
Kowianski P., Karwacki Z., Ludkiewicz B., Domaradzka-Pytel B., Wojcik S., Morys J., Narkiewicz O.
Acta Neurobiologiae Experimentalis
|
2001
|
tom 61
|
nr 3
10

Localization of the visual area in the claustrum of the rat, rabbit and cat

76%
Zielinski P., Morys J., Kowianski P., Karwacki Z., Sadowski M., Sloniewski P., Narkiewicz O.
Acta Neurobiologiae Experimentalis
|
1995
|
tom 55
|
nr 5
11

The pathophysiology of intracerebral haemorrhage

76%
Karwacki Z., Kowianski P., Witkowska M., Karwacka M., Dziewiatkowski J., Morys J.
Folia Morphologica
|
2006
|
tom 65
|
nr 4
295-300
Spontaneous intracerebral haemorrhage carries a high mortality rate and treatment of the disease raises more questions then answers. Mass effect, ischaemia and toxicity of blood components are responsible for brain tissue damage. Initially occurring disturbances of cerebral blood flow have a temporary character and do not play a key role in the pathology of intracerebral haematoma. Oedema formatting in the 24–48 hours after intracerebral bleeding is the result of multidirectional processes. The pathological mechanism that underlines it is the function of activation of systemic complement and cascade of coagulation. In the light of these findings, further clinical and experimental investigations should be focused on these factors.
12

Neurons of the claustrum projecting to the somatosensory and motor cortical areas in the rabbit

76%
Kowianski P., Morys J., Zielinski P., Karwacki Z., Sadowski M., Narkiewicz O.
Acta Neurobiologiae Experimentalis
|
1995
|
tom 55
|
nr 5
13

Neurons of the claustrum projecting to the motor and somatosensory cortex in the rabbit - assessment with the method of retrograde transport of fluorescent tracers

76%
Kowianski P., Morys J., Dziewiatkowski J., Karwacki Z., Bobek-Billewicz B., Narkiewicz O.
Folia Morphologica
|
1996
|
tom 55
|
nr 4
14

Claustrocingulate connections in the rabbit and rat - a stereological study

76%
Majak K., Kowianski P., Dziewiatkowski J., Karwacki Z., Luczynska A., Morys J.
Folia Morphologica
|
2000
|
tom 59
|
nr 1
Unbiased stereological methods were used for estimating the numerical density and the total number of claustral neurones projecting to the cingulate cortex in rabbit and rat. In rat the numerical density of neurones projecting to the retrosplenial granular cortex (RSG) differed significantly from those projecting to the retrosplenial agranular (RSA) and cingulate (Cg) cortices while in rabbit the numerical densities of retrogradely labelled neurones in the claustrum following injections into various areas of the cerebral cortex did not differ significantly. The total number of retrogradely labelled neurones in the claustral limbic zones did not differ significantly in both species. The quantitative analysis of claustral zones projecting to a different cingulate cortex area, both in rabbit and rat, reveals that each of these zones is rather homogeneous.
15

Apoptosis in the course of experimental intracerebral haemorrhage in the rat

64%
Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.
Folia Morphologica
|
2005
|
tom 64
|
nr 4
248-252
Intracerebral haematoma was produced in 25 adult rats by infusion of 100 µl of autologous blood into the striatum. The animals’ brains were removed at 1, 3, 7, 14 and 21 days after production of the haematoma. The TUNEL method was used to detect DNA fragmentation and TUNEL-positive cells were qualified. TUNEL-positive cells were already found on the first day of observation and were present for three weeks after haematoma production. These results provide evidence that programmed cell death is associated with intracerebral haemorrhage.
16

Dynamics of development and morphology of reactive astrogliosis in response to one hour transient cerebralischemia in the rat-immunohistochemical studies

64%
Steliga A., Lietzau G., Waskow M., Karwacki Z., Wojcik S., Sienkiewicz W., Morys J., Kowianski P.
Acta Neurobiologiae Experimentalis
|
2015
|
tom 75
|
nr Supl.
BACKGROUND AND AIM: Transient focal cerebral ischemia is stimulus triggering reactive astroglial response characterized as the cellular proliferation and restoring of differentiation abilities necessary for reduction of the consequences of primary brain lesion and separation of ischemia. Growing evidence of data point to specific, context-dependent character of this response dependent from the pathological stimuli, intensity of lesion and time-lapse from the beginning of the pathological process. In our study we aimed to assess the potential for proliferation and differentiation of reactive astroglia after transient focal brain ischemia in the rat. METHODS: Transient focal cerebral ischemia was evoked in 20 adult Wistar rats by placement of the surgical thread into the carotid artery and occlusion of the middle cerebral artery for 1 h. The post operative survival period was up to 6 weeks. Immunocytochemical double-stainings for glial fibrillary acidic protein (GFAP), with proliferative (Ki-67 and Pax6,) and characteristic for various stages of gliogenesis (nestin and S100beta protein) markers were performed, with subsequent confocal microscopic study. RESULTS: The apparent differences in the pattern of colocalization within GFAP-immunorecative (ir) astrocytes were observed in the survival period. The intensity of double-labeling for the studied markers increased gradually after 24 h after initiating the ischemia. Intense hypertrophic reaction and increased concentration of GFAPir fibers was observed especially after two weeks of reperfusion. CONCLUSION: The astroglial reaction in the course of reperfusion indicate regaining of the morphological features, characteristic for the earlier developmental stages, as well as the development of proliferative capacities. The considerable potential of astroglial proliferative response even after short-lasting transient cerebral ischemia must be taken into account in the future studies of cerebral infarcts.
17
Content available remote

The influence of sevoflurane on the reactivity of astrocytes in the course of the experimental intracerebral haemorrhage in rat

64%
Karwacki Z., Kowianski P., Dziewiatkowski J., Domaradzka-Pytel B., Ludkiewicz B., Wojcik S., Narkiewicz O., Morys J.
Journal of Physiology and Pharmacology
|
2005
|
tom 56
|
nr 3
40 adult Wistar rats were divided into two groups depending on the applied anaesthesia. In both groups animals were generally anaesthetized with fentanyl, dehydrobenzperidol administered intraperitoneally and midazolam given intramuscularly. In the second group (SEVO) animals received sevoflurane of 2.2 vol% end-tidal concentration. Intracerebral haematoma was produced through infusion of 100 µl of autologous blood into the striatum. Each group was divided into five subgroups depending on the length of survival period: 1, 3, 7, 14, 21 days. The astrocytic population was studied by means of anti-GFAP staining. Stereological analysis was applied to estimate the numerical density of immunoreactive cells and the distribution of their types. On 7th day of observation the density of GFAP-immunoreactive astrocytes in SEVO was lower (p<0,05) than that in the control group. In the control group, the increase (p<0.05) of per cent of activated astrocytes between the 1st and 3rd survival day was noted, which remained at this level till the end of observation. In SEVO group, the increase (p<0.05) of per cent of activated astrocytes between the 3rd and 7th day and the decrease (p<0.05) between the 14th and 21st survival day were observed. During days of observation the per cent of activated astrocytes was lower (p<0.05) in the SEVO group than that in the control group. Administration of sevoflurane during anaesthesia to animals with intracerebral haemorrhage has evoked not only the delay of the activation of astrocytes but also decrease in its level.
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