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1

Analysis of Pasteurella multocida strains isolated from cattle with haemorrhagic septicaemia by the use of PFGE

100%
Kedrak-Jablonska A., Borkowska-Opacka B.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 4
2

Electrophoretic profiles of the outer membrane proteins of Pasteurella multocida strains isolated from cattle

100%
Kedrak-Jablonska A., Borkowska-Opacka B.
Bulletin of the Veterinary Institute in Puławy
|
2006
|
tom 50
|
nr 4
3

Molecular characterisation of Pasteurella multocida strains isolated from calves by pulsed - field gel electrophoresis

100%
Kedrak-Jablonska A., Borkowska-Opacka B.
Bulletin of the Veterinary Institute in Puławy
|
2007
|
tom 51
|
nr 4
4

Badania nad zastosowaniem metody western-blotting do serologicznej diagnostyki nasacizny

71%
Kedrak-Jablonska A., Budniak S., Szczawinska A., Reksa M., Krupa M.
Medycyna Weterynaryjna
|
2013
|
tom 69
|
nr 02
The aim of the study was to evaluate the application of SDS-PAGE electrophoresis and western-blotting for serological diagnostics of glanders. Six commercial Burkholderia mallei antigens, eight anti-B. mallei sera and two negative sera were used in the study. In the first part of the study electrophoretic profiles of antigens in SDS-PAGE were determined at a gradient of 4% to 20% of polyacrylamide gel. Optimal conditions of western-blotting reaction were determined. In the case of all examined antigens and anti-B. mallei sera, the presence of protein fractions in the range from 42 to 33 kDa and one band of the molecular weight of 16 kDa were demonstrated by immunoblotting reaction. Additional bands of the different molecular weight were also observed. The antigens examined did not show reaction with negative sera. In the next part of the study, a sensitivity of the western-blotting method and the CFT were compared. The western-blotting method was two - four times more sensitive than the CFT.
5

Pastereloza królików - aktualny stan wiedzy

71%
Budniak S., Kedrak-Jablonska A., Szulowski K., Szczawinska A., Reksa M.
Medycyna Weterynaryjna
|
2012
|
tom 68
|
nr 03
Pasteurellosis in rabbits is caused by the bacterium Pasteurella multocida. The serotypes 3 or 12 strains of P. multocida, representing diverse virulence and pathogenicity, are most frequently separated from rabbits. P. multocida causes a variety of clinical syndromes, including acute and chronic rhinitis, pneumonia, otitis media, infections of the genital tract, localized abscesses and septicemia. The fight against pasteurellosis is based on two strategies: elimination of carriers and sick animals from breeding and the improvement of environmental conditions. As the disease is caused by various serotypes, vaccination becomes difficult. Immunity against one serotype does not necessarily result in protection against a heterologous serotype. A detailed description of the ethiopatology, clinical and anatomopathological manifestations, diagnosis, therapy and control of the disease has been presented in the paper.
6

Zastosowanie metody Real-time PCR opartej na fluorescencji barwnika SYBR Green I do wykrywania genów zlokalizowanych w DNA plazmidów pXO1 i pXO2 oraz specyficznej sekwencji chromosomalnej rpoB szczepów Bacillus anthracis

71%
Budniak S., Kedrak-Jablonska A., Reksa M., Szczawinska A., Borkowska-Opacka B.
Medycyna Weterynaryjna
|
2010
|
tom 66
|
nr 09
s.630-634,rys.,tab.,bibliogr.
The aim of the study was to apply the real-time PCR method with the intercalating dye SYBR Green I for the detection of genes of Bacillus anthracis located on plasmids pXO1 and pXO2 and the specific chromosomal rpoB sequence. The research was conducted on one vaccinal and four field strains of Bacillus anthracis. The assessment of the specificity of the tests involved isolates of other species of the genus Bacillus as well as strains of six other species of microorganisms. The studies were conducted with the PCR QuantiTect kit (Qiagen) and primers specific for the gene pag coding PA protein, gene cap coding capsule, and primers for the amplification of the specific chromosomal sequence. PCR enabled the detection of all genes under examination by the observation of amplification curves. The specificity of real-time PCR was confirmed by estimating melting temperatures of PCR products. It was shown that the melting temperatures of amplicons obtained in the reaction were 78°C, 79°C and 76°C in cases of detecting the chromosomal rpoB sequence, pag gene, and cap-C gene, respectively. The sensitivity and linearity of the reactions were determined using a regression coefficient. A high regression coefficient of 0.99 was demonstrated for all the reactions. The real-time tests were highly sensitive and specific.
7

Comparison of two multiplex PCR assays for the detection of Listeria spp. and Listeria monocytogenes in biological samples

61%
Budniak S., Kedrak-Jablonska A., Szczawinska A., Reksa M., Krupa M., Szulowski K.
Journal of Veterinary Research
|
2016
|
tom 60
|
nr 4
8

Isolation and identification of Pasteurella multocida from European bison in Poland

61%
Kedrak-Jablonska A., Budniak S., Szczawinska A., Reksa M., Krupa M., Krzysiak M., Szulowski K.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 3
9

Evaluation of Real-time PCR based on TaqMan probes for identification of Bacillus anthracis in biological samples

61%
Budniak S., Kedrak-Jablonska A., Krupa M., Szczawinska A., Reksa M., Szulowski K.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 3
10

The use of Real-time PCR based on the fluorescence of SYBR Green I for identification of Bacillus anthracis in biological samples

61%
Kedrak-Jablonska A., Budniak S., Krupa M., Szzcawinska A., Reksa M., Szulowski K.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 3
11

Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

61%
Kedrak-Jablonska A., Budniak S., Krupa M., Szczawinska A., Reksa M., Szulowski K., Iwaniak W.
Journal of Veterinary Research
|
2017
|
tom 61
|
nr 4
12

Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

61%
Kedrak-Jablonska A., Budniak S., Szczawinska A., Reksa M., Krupa M., Szulowski K.
Journal of Veterinary Research
|
2018
|
tom 62
|
nr 4
13

Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

61%
Kedrak-Jablonska A., Budniak S., Szczawinska A., Reksa M., Krupa M., Szulowski K.
Bulletin of the Veterinary Institute in Puławy
|
2015
|
tom 59
|
nr 4
The aim of the study was the application and comparison of real-time PCR methods based on the fluorescence of SYBR Green I intercalating dye and TaqMan probes for the detection of the 23S rDNA gene of Listeria spp. and the hlyA gene of Listeria monocytogenes. Five strains of L. monocytogenes and single strains of each of the species: L. ivanovii, L. innocua, L. grayi, L. welshimeri, and L. seeligeri were used for the experiments. Additionally, five strains of other species of bacteria were used for evaluation of the specificity of the tests. QuantiTect SYBR Green PCR and QuantiTect Probe PCR kits were selected for the study. In the first stage of the study, SYBR Green I real-time PCRs were performed under several methods, the first one allowing detection of the 23S rDNA gene and the remainder based on the amplification of the hlyA gene. In the next part, three varied in method TaqMan probe-based real-time PCRs allowing confirmation of strains belonging to Listeria spp. and L. monocytogenes were conducted. The observation of amplification curves in real-time PCR methods enabled the detection of both genes, and these methods demonstrated a significant sensitivity and high specificity. A high regression coefficient of 0.99 was found for all reactions. Specific amplification products were obtained for the 23S rDNA and hlyA genes, which confirmed the tested strains as Listeria spp. and L. monocytogenes respectively. Isolates of other microbial species did not yield real-time PCR products.
14

Isolation of Klebsiella pneumoniae and Klebsiella oxytoca from invasive turtle species in Poland

61%
Budniak S., Kedrak-Jablonska A., Szczawinska A., Reksa M., Krupa M., Szulowski K.
Postępy Mikrobiologii. Suplement
|
2017
|
tom 56
|
nr 3
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