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Effect of homogenization and ultrasonication on the physical properties of insoluble wheat bran fibres

100%
Hu R., Zhang M., Adhikari B., Liu Y.
International Agrophysics
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2015
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tom 29
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nr 4
2

Effects of overexpression of four Populus wound-inducible genes in Arabidopsis on its resistance against Plutella xylostella L

76%
Chen S., Wang J., Hu R., Song Y., Yang S., Zhang L.
Acta Physiologiae Plantarum
|
2012
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tom 34
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nr 4
Wounding- and herbivore-inducible proteins are potential resources to enhance the resistance to insect pests in transgenic plants. In order to analyze the effects of four such highly inducible genes of Populus, two genes encoding trypsin inhibitors (PtdKTI5 from hybrid poplar, Populus trichocarpa × P. deltoides and PtKTI2 from trembling aspen, Populus tremuloides Michx.), a woundinducible gene (PtdKTI5 from hybrid poplar, Populus trichocarpa × P. deltoids) and a stress-responsive gene (PtdPOP3 from hybrid poplar, Populus trichocarpa × P. deltoids), we have produced transgenic Arabidopsis thaliana expressing these genes individually under the control of the CaMV35S promoter by Agrobacterium-mediated transformation. Stable integration and high transcriptional levels of the target cDNAs and their inheritance in transgenic Arabidopsis lines were confirmed by genomic PCR, quantitative PCR and T₃ progeny segregation analysis. In the no-choice bioassays, all the four types of transgenic Arabidopsis confer different levels of insect resistance against Plutella xylostella L. larvae at various stages and the PtdKTI5 overexpressing plants turned out to be most effective. Our data indicated that these four Populus defense-related genes under investigation could be potentially exploited for the protection against P. xylostella L. in transgenic plants.
3

Analysis of heavy metal sources and health risk assessment of typical coal mine collapsed lakes in Huaibei Coalfield, Anhui Province, China

64%
Li J., Gui H., Hu R., Chen L., Xia Y., Fan H., Yu H., Wang M.
Polish Journal of Environmental Studies
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2020
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tom 29
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nr 5
4

Comparative proteomic analysis reveals differential protein and energy metabolisms from two tobacco cultivars in response to cold stress

64%
Hu R., Zhu X., Xiang S., Zhang X., Liu Z., Zhu L., Cao Y., Yang C., La J.
Acta Physiologiae Plantarum
|
2018
|
tom 40
|
nr 01
Low temperature is an important abiotic stress for plant development and has serious effects on crop production. Because tobacco is sensitive to low temperature, it is suitable for analyzing the mechanisms of cold response in plants. In the current study, NC567 and Taiyan8, two cultivars with different sensitivities to low temperature, were used in Isobaric Tags for Relative and Absolute Quantitation (iTRAQ)-based proteomics to uncover their different mechanisms in response to cold stress. A total of 4317 distinct proteins were identified and the differentially expressed proteins in four comparison sets were used for further analysis. The gene ontology (GO) analysis indicated that the majority of differentially expressed proteins were in the categories involved in metabolic and cellular processes. Surprisingly, there were 55 proteins decreased in NC567, but increased in Taiyan8 in response to cold, while the levels of 42 proteins were lower in Taiyan8 than NC567 at normal temperature, but higher in Taiyan8 than NC567 under cold treatment, suggesting different responses to cold stress in these cultivars. The levels of polypeptides involved in protein synthesis and degradation, photosynthesis, and respiration, as well as ROS scavenging, were different in the comparison sets, implying that protein and energy metabolisms may be important for the establishment of cellular environment at low temperature. In conclusion, our study identified the potential pathways involved in low-temperature response of tobacco and provides hints for the further improvement of cold tolerance in crops.
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