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1
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Laboratory diagnosis of mycoplasma infection in young cattle

100%
Gabinaitiene A., Siugzdaite J., Zilinskas H.
Polish Journal of Veterinary Sciences
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2011
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tom 14
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nr 1
The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.
2

Comparative molecular characterisation of the rabies virus in the Lithuanian raccoon dog population

100%
Zienius D., Zilinskas H., Sajute K., Stankevicius A.
Bulletin of the Veterinary Institute in Puławy
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2009
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tom 53
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nr 2
179-182
The objective of this work was to determine nucleoprotein gene sequences and genetically characterise the rabies virus (RABV) isolates in order to know which virus group (biotype) is circulating in the raccoon dog population in Lithuania. For rabies virus phylogenetic characterisation, 16 RABV isolates from raccoon dogs were used. The isolates were selected according to different geographical location. A 400 bp region of the nucleoprotein gene was sequenced and analysed together with reference raccoon dog sequences of the RABV isolates from Estonia, Latvia, Russia, Poland, and other West European countries available in the GenBank. Phylogenetic analysis revealed that the isolates from different parts of Lithuania were closely related and belonged to RABV genotype 1 and showed significant bootstrap support inside North-Eastern Europe group of raccoon dog RABV. The phylogenetic relationships between Lithuanian raccoon dogs RABV isolates and those from neighbouring countries showed 97.7%- 99.5% identity. A close genetic relationship between the raccoon dog and fox rabies isolates from different regions of Lithuania (99.5%) was identified, suggesting that rabies viruses circulating in raccoon dogs and foxes in this region might have the same origin.
3

Effect of synthetic GnRH analogues on ovarian follicular growth dynamic and oestrus

100%
Masiulis M., Zilinskas H., Rutkauskas A., Riskeviciene V.
Medycyna Weterynaryjna
|
2007
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tom 63
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nr 08
922-925
The aim of the present study was to evaluate the stimulating effect of synthetic GnRH analogues without the use of prostaglandins on ovarian follicular growth dynamic and oestrus. The study was made on 30, 3 to 9-year-old Lithuanian Black and White (LBW) and German Black and White (GBW) breed cows. The study was conducted in the Lithuanian Veterinary Academy practical training and research farm. Oestrus in 23 cows was stimulated using synthetic GnRH analogues Dalmarelin (Lecirelin) (Fatro S. p. A., Italy) and in 14 cows with the use of Depherelin® (Gonavet® 50, Veyx-Pharma GmbH, Germany). Dalmarelin (Lecirelin) induced oestrus in 100% of the stimulated cows, and Depherelin® (Gonavet® 50) induced oestrus in 92.9 % of stimulated cows. Following an injection of synthetic GnRH analogues, preparations Depherelin® and Dalmarelin, plasma progesterone concentration decreased, due to the ovulation of dominant follicle or onset of the new follicular wave. Cows stimulated with Dalmarelin had a faster follicular growth rate and bigger size of follicles at ovulation compared to Depherelin® and control group cows (p = 0.05). In the pregnancy trial that followed 45 days after artificial insemination, the highest percentage (43.5 %) of pregnant cows was determined in the group of cows stimulated with Dalmarelin.
4

Comparison of in vitro methods for evaluation of boar semen quality

100%
Serniene L., Riskeviciene V., Banys A., Zilinskas H.
Medycyna Weterynaryjna
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2005
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tom 61
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nr 03
5

Persistence of European and American type PRRSV strains within Lithuanian pig herd

88%
Stankevicius A., Cepulis R., Zilinskas H., Stadejek T., Pejsak Z.
Bulletin of the Veterinary Institute in Puławy
|
2008
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tom 52
|
nr 3
The objective of this study was to evaluate the diversity and persistence of porcine reproductive and respiratory syndrome virus (PRRSV) in a swine herd where American type of modified live vaccine (MLV) was used. The study was carried out on the farrow-to-finish farm with 2 500 sows, which had a history of endemic PRRSV manifested as periodic mini-outbreaks of PRRS. The farm has been endemically infected with PRRSV-EU since 1998. The vaccination programme with the MLV was first applied in 2001 and consisted of vaccinating all sows every six months and all gilts 60 d before farrowing. All incoming gilts were also vaccinated twice with a two-week interval. During the monitoring period, PRRSV-EU type was detected in 28 of 63 samples collected from two to three-month-old piglets. PRRSV-US type was detected in 20 samples. Nineteen samples were positive for both EU and US types of PRRSV at the same time. The results of RT-PCR testing serum samples from 58 sows were negative. ORF5 RT-PCR products from samples containing both PRRSV-EU and PRRSV-US types were sequenced. Phylogenetic analyses, showed a close relationship to the American genotype of PRRSV strains from the monitored farm to the known vaccine strain V2332 present in the American type PRRS MLV. The presence of the PRRSV-US in the two-three-month-old piglets indicates that the American type of vaccine virus has spread from vaccinated sows to the non-vaccinated piglets. Simultaneous presence of both PRRSV-EU and US strains in 19 samples suggests very low cross protection between the American type PRRS MLV and very diverse Lithuanian EU type field strains. In this situation an increased chance for inter-genotypic recombination can be a threat.
6

Assessment of factors influencing the culling rate of boars with reproductive disorders in Lithuanian AI Centres

88%
Serniene L., Riskeviciene V., Banys A., Januskauskas A., Zilinskas H.
Medycyna Weterynaryjna
|
2005
|
tom 61
|
nr 09
7

Influence of gonadotropin treatment after insemination on dairy cows to maintain pregnancy, and milk progesterone concentration

76%
Juodzents V., Gavelis A., Juozapavicius A., Zilaitis V., Juozaitiene V., Zilinskas H.
Medycyna Weterynaryjna
|
2017
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tom 73
|
nr 08
The aim of the study is to evaluate the possibility of maintenance of cow pregnancy applying GnRH treatment after AI. The experiment was carried out on Lithuanian black-white lactating dairy cows on 90-100 DM. Were selected 45 cows on their 2nd and 3rd lactation with milk yield of 8.500-9.000 kg. The cows with 16 mm or greater (n = 26) CL in ovaries were selected for the experiment. All cows were given the injections of dinoprost solution 25 µg. AI was performed on the cows (n = 20) that showed oestrus. The first group (experimental) of cows (n = 10) were repeatedly given the injections of gonadorelin 100 µg im on the 7th day after AI. The second group (control) of cows (n = 10) were given the injections of 0.9% saline solution on the 7th day after AI. The ultrasound measurement was performed to determine the size of ovaries, and CL’s. Concentration of P4 were detected in milk samples. Ultrasound testing as well as milk P4 determination were carried out 4 times in total, every 7 days starting on the day of AI. After 7 days following the injection of GnRH, and positive increase of the number of cows with accessory CL by 56% was determined, comparing with the second (control) group. The tendency of increasing size of CL in both groups after AI was noticed. The changes in the ovary size were similar in both groups. The average number of CL in the first group was by 36% (P < 0.05) higher than in the second group (1.5 and 1.1). The change of P4 was similar in both groups. The number of pregnant cows in the first group was by 10% higher than in the second group.
8
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Influence of puerperal metritis on the first ovulation after calving in dairy cows

76%
Juodzentis V., Siugzdaite J., Palubinskas G., Juodzente D., Rekesiute A., Zilinskas H.
Polish Journal of Veterinary Sciences
|
2020
|
tom 23
|
nr 4
9

Evaluation of efficacy of selected estrous synchronization programs depending on the functional state of the ovaries, heifers and cows of meat breed

76%
Rekesiute A., Januskauskas A., Kerziene S., Sutkeviciene N., Vaiciuliene G., Rutkauskas A., Zilinskas H.
Journal of Animal and Feed Sciences
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2020
|
tom 29
|
nr 2
10
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Agreement of different methods for assessment of progesterone concentrations in beef cattle serum

64%
Rekesiute A., Januskauskas A., Siukscius A., Riskeviciene V., Vitkauskiene A., Verkauskiene, Antanaitis R., Zilinskas H.
Polish Journal of Veterinary Sciences
|
2019
|
tom 22
|
nr 4
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