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The presence of antibodies against IBR/IPV (BHV-1), BVD-MD and PI-3 was serologically examined in sera of cows and heifers after abortion. It was found that the greater number of animals have antibodies against PI-3 (40.4%). Antibodies against IBR/IPV were found in 26.8% and against BVD-MD in 19.6% of animals. The obtained results suggest that infections caused by these viruses are the risk factors in cows as causes of disturbances in reproduction.
Medycyna Weterynaryjna
|
2008
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tom 64
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nr 09
1118-1121
Aujeszky’s disease (AD) also know as pseudorabies is an acute and usually fatal disease of pig by Herpesvirus suis type 1. The purpose of the research was to evaluate the epidemiological situation of AD in the Gdañsk coast area (Poland) on the basis of serological examination of pigs and cases of AD in other domestic species. Serological examination for the presence of antibodies to Aujeszky’s disease virus (ADV) were performed using the SN method and ELISA test. ADV isolation was also attempted from the tissue of dead animals. Isolated strains of ADV were characterised by PCR-RFLP. Serological investigation revealed the presence of ADV antibodies in 281 (4.4%) pig serum samples. The mean percentage prevalence of antibodies in swine from large farms, small farms and copulation stations (boars) were 3.5%, 4.9% and 12.5% respectively. The highest (9.8%) prevalence of ADV antibodies was found in 1996. Furthermore, this paper describes the outbreak of AD among domestic animals on a single farm that led to the deaths of seven cattle, three goats, three sheep, two cats and one dog, all of them with CNS signs. The animals were housed in a separate building away from swine. ADV strains were isolated from the tissue of each animal species. PCR-RFLP analysis revealed that the five ADV strains had the identical profile, which suggests that the animals were infected by the same ADV strain. In conclusion, this case of AD in several domestic species demonstrates the danger from latently infected swine.
An outbreak of Aujeszky's disease (AD) occurred in a herd of domestic animals that led to the death of seven cattle, three goats, three sheep, two cats and one dog, all of them with CNS signs. The animals were not in direct contact with swine. The ADV was detected in the tissue of affected animals by celi culture methods and PCR. Genome strains of ADV were characterized by restriction en- donuclease analysis using BamH I. The results indicated that the strains of virus were identical and belonged to the type genome I of AD. Compared with vaccine and isolated strains obtained from the pig in the same region, considerable differences in DNA patterns were detected. Interestingly, the strains isolated from the dead animals were similar to Buk T-900 reference strains.
For detecting antibodies against IPV-IBR there were used two serological methods, i.e. the virus neutralization and ELISA tests. The tests were compared using 1277 samples of blood. A high degree of correlation between two methods was observed. However, the ELISA proved to be more sensitive and therefore it should be applied particularly during mass control examinations of cattle.
Maedi virus has been isolated from sheep and serological tests, cytopathological data and biochemical examinations confirmed that the isolated virus was the maedi one. Viral particles were visible under an electron microscope in negatively stained samples prepared from the supernatant of infected cell cultures. The size of the particles corresponded to an average size of the maedi virus. The findings confirmed previous clinical, epizootic and serological data with regard to the existence of maedi disease in Poland.
The purpose of the studies was to assess the spread of BHV-1 infection in dairy cows. Clinical, virological and serological examinations were carried out in two stages within four years. Numerous clinical and asymptomatic cases of pustular vulvovaginitis were found in the herds under study. Serologic results showed that all the herds were infected (4.9 - 56.6%). The findings revealed a higher dynamics of infection spread in herds with low numbers of infected animals than in those with a high percentage of cows having specific antibodies against BHV-1. The results indicated that most cows aged 3-5 years had SN antibodies against BHV-1.
Анализировали потери, учитывающие падеж и вынужденный забой больных овец с симптомами адеиоматоза и болезни maedi поморской и мери- носской пород в 1981—1983 гг. Полученные резуль­таты исследований подверли статистическому ана­лизу- с использованием .ЭВМ. В исследуемом пе­риоде потери овец составляли 27,18% (1025/3771) среди поморской породы и 4,29% (68/1585) породы меринос. Вынужденный забой овец поморской по­роды отличался большей регулярностью, падеж же имел случайный характер. Сезонность потерь по­морской породы отличается большей интенсив­ностью с января по май, породы же меринос — в осенний период с интенсивностью в ноябре.
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