Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Limited GADD45 alpha expression and function in IL-1 beta toxicity towards insulin-producing cells

100%

Skalniak L., Gurgul-Convey E., Okreglicka K., Skalniak A., Jura J.

Acta Biochimica Polonica

2013

tom 60

nr 4

Growth arrest and DNA damage-inducible (GADD) 45 proteins are regulators of cell death and survival. The proinflammatory cytokine IL-1β strongly increases the level of the transcript encoding GADD45α in rat insulin-producing INS-1E cells. The activation of Gadd45α gene is clearly dependent on JNK and NF-κB activation and the synthesis of the secondary mediator nitric oxide (NO). Interestingly, the observed twelve-fold increase in the GADD45α-coding transcript level is not followed by increased expression of GADD45α at the protein level. An analysis of IL-1β toxicity in INS-1E cells overexpressing GADD45α revealed no correlation between the GADD45α protein level and the sensitivity to IL-1β toxicity. These findings suggest that the potential engagement of GADD45α in IL-1β toxicity towards beta cells is limited to the effects induced by the basal expression level of this protein.

Ograniczanie wyników

1 Acta Biochimica Polonica

1 Gurgul-Convey E.

1 Jura J.

1 Okreglicka K.

1 Skalniak A.

1 Skalniak L.

1 2013

Wyniki wyszukiwania

Limited GADD45 alpha expression and function in IL-1 beta toxicity towards insulin-producing cells