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Thirteen candidate genes for human obesity were selected for cytogenetic mapping by FISH in the pig genome. Among them, 6 genes were assigned to chromosomes for the first time (NR3C1, GNB3, ADRB1, ADRB2, ADRB3 and UCP1). Location of the other 7 genes (INSIG2, LIPIN1, PLIN, NAMPT, ADIPOQ, UCP2 and UCP3), earlier mapped by somatic cell hybridization or with the use of a radiation hybrid panel, was verified (INSIG2) or more precisely described. The genes were assigned to the following chromosomes: INSIG2 to SSC15q12, LIPIN1 to SSC3q26, NR3C1 to SSC2q29, PLIN to SSC7q15, GNB3 to SSC5q21, NAMPT to SSC9q23, ADIPOQ to SSC13q41, ADRBl to SSC14q28, ADRB2 to SSC2q29, ADRB3 to SSC15q13-14, UCP1 to SSC8q21-22, and both UCP2 and UCP3 to SSC9p24. Most of the genes were located within known QTL for pig fatness traits.
Our article describes a study on the influence of mineral and organic fertilisation and three strains of Trichoderma sp. isolated from Polish soils on the count of moulds, including the Fusarium and Alternaria genera, as well as the soil fertility index and the yield of red beets. Apart from that, the aim of the study was to assess the interaction between Trichoderma sp. and plant pathogens. A field experiment was performed on haplic luvisols soil in 11 combinations: a control sample, a variant with a mineral fertiliser, one with manure and eight variants where tomato or onion waste composts were applied. Some of them were inoculated with Trichoderma atroviride (T1) and/or T. harzianum (T2 and T3) isolates. The total count of moulds and the counts of Trichoderma sp., Fusarium sp., and Alternaria sp. in the soil were the most strongly influenced by the addition of mineral fertiliser and manure. On the other hand, the values of the soil fertility index were the highest in the soil enriched with manure, followed by the soil combinations with tomato waste composts. Vegetable waste composts applied to the soil proved to be good carriers of Trichoderma sp. isolates. The study did not prove any antagonistic effect of Trichoderma sp. isolates on the proliferation of Alternaria sp. The growth and development of Fusarium sp. was inhibited by the Trichoderma – T3 strain on its own or in combination with isolate Trichoderma – T1. The yield of red beet roots and leaves was the highest in the combination with the mineral fertiliser and in the soil fertilised with the composts that had been simultaneously inoculated with two strains, i.e., Trichoderma – T1 and Trichoderma – T2, or Trichoderma – T1 and Trichoderma – T3.
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