A serological study of BoHV-1 distribution was conducted in Lithuania from 2005 to 2009. Antibody level was measured using a commercial ELISA. For serological examination, 15,368 random blood samples from cattle of different age, gender, and size of herd, which was unvaccinated against IBR, were collected in 37 districts. It was registered that 11.97% of BoHV-1 were seropositive samples. It was also shown that BOHV-1 is most widespread in cattle herds with population >200 individuals (14.79%). Comparison of different sex groups of cattle revealed that the highest number of infected animals was identified in cows (34.64%) and the lowest in bulls (2.01%). In heifers the number of infected animals was 10.01% and in calves - 4.41%. It was shown that seroprevalence of BoHV-1 infection in Lithuania increased with age of animals. The highest prevalence of BoHV-1 (53.98%) was registered in cattle aged more than 7 years.
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