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Housing conditions influence motor functions and exploratory behavior following focal damage of the rat brain

100%

Gornicka-Pawlak E., Jablonska A., Chylinski A., Domanska-Janik K.

Acta Neurobiologiae Experimentalis

2009

tom 69

nr 1

The present study investigated influence of housing conditions on motor functions recovery and exploratory behavior following ouabain focal brain lesion in the rat. During 30 days post-surgery period rats were housed individually in standard cages (IS) or in groups in enriched environment (EE) and behaviorally tested. The EE lesioned rats showed enhanced recovery from motor impairments in walking beam task, comparing with IS animals. Contrarily, in the open field IS rats (both lesioned and control) traveled a longer distance, showed less habituation and spent less time resting at the home base than the EE animals. Unlike the EE lesioned animals, the lesioned IS rats, presented a tendency to hyperactivity in postinjury period. Turning tendency was significantly affected by unilateral brain lesion only in the EE rats. We can conclude that housing conditions distinctly affected the rats behavior in classical laboratory tests.

Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells being at different level of neural commitment

64%

Gornicka-Pawlak E., Janowski M., Habich A., Jablonska A., Drela K., Kozlowska H., Lukomska B., Sypecka J., Domanska-Janik K.

Acta Neurobiologiae Experimentalis

2011

tom 71

nr 1

The aim of the study was to evaluate therapeutic effectiveness of intra-arterial infusion of human umbilical cord blood (HUCB) derived cells at different stages of their neural conversion. Freshly isolated mononuclear cells (D-0), neurally directed progenitors (D-3) and neural-like stem cells derived from umbilical cord blood (NSC) were compared. Focal brain damage was induced in rats by stereotactic injection of ouabain into dorsolateral striatum Three days later 107 of different subsets of HUCB cells were infused into the right internal carotid artery. Following surgery rats were housed in enriched environment for 30 days. Behavioral assessment consisted of tests for sensorimotor deficits (walking beam, rotarod, vibrissae elicited forelimb placing, apomorphine induced rotations), cognitive impairments (habit learning and object recognition) and exploratory behavior (open field). Thirty days after surgery the lesion volume was measured and the presence of donor cells was detected in the brain at mRNA level. At the same time immunohistochemical analysis of brain tissue was performed to estimate the local tissue response of ouabain injured rats and its modulation after HUCB cells systemic treatment. Functional effects of different subsets of cord blood cells shared substantial diversity in various behavioral tests. An additional analysis showed that D-0 HUCB cells were the most effective in functional restoration and reduction of brain lesion volume. None of transplanted cord blood derived cell fractions were detected in rat's brains at 30th day after treatment. This may suggest that the mechanism(s) underlying positive effects of HUCB derived cell may concern the other than direct neural cell supplementation. In addition increased immunoreactivity of markers indicating local cells proliferation and migration suggests stimulation of endogenous reparative processes by HUCB D-0 cell interarterial infusion.

Ograniczanie wyników

2 Acta Neurobiologiae Experimentalis

2 Domanska-Janik K.

2 Gornicka-Pawlak E.

2 Jablonska A.

1 Chylinski A.

1 Drela K.

1 Habich A.

1 Janowski M.

1 Kozlowska H.

1 Lukomska B.

1 Sypecka J.

1 2011

1 2009

Wyniki wyszukiwania

Housing conditions influence motor functions and exploratory behavior following focal damage of the rat brain

Systemic treatment of focal brain injury in the rat by human umbilical cord blood cells being at different level of neural commitment