The following toxic compounds were administered to the biotest with the application of the protozoan Spirostomum ambiguum as the test object: sodium cyanide, isopropylic methanophosphoric acid ester fluoride (sarin) and phosphoric acid 0-ethylo n-dimethyloamid ester cyanide (tabun). For the purpose of the LCS0 calculations, i.e. the concentrations of the sodium cyanide, sarin and tabun causing death in 50% of the tested Spirostomum ambiguum after an established period of test time, the Münch Reed method as well as as the B-splines, cubic splines and the Bezier curves were used. Calculations have also been made with regard to the average LCS0 concentrations of the analyzed compounds, the standard deviations and confidence intervals. The behavior of Spirostomum ambiguum was observed, establishing the time of death of two organisms. The measurement was carried out 21 times for each concentration and compound in three measuring cycles. On the basis of the data base from the biotest, the following have been proposed: the established LCso values, standard deviations and confidence intervals, the identification of an unknown toxic compound existing in water and determining the concentration of this compound. It has been concluded that Spirostomum ambiguum satisfies the requirements imposed on test animals for the purpose of bioanalysis of sodium cyanide, sarin and tabun.
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