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1
Artykuł dostępny w postaci pełnego tekstu - kliknij by otworzyć plik
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Association between SOX5 genotypes and semen quality in Polish Holstein-Friesian bulls

100%
Hering D. M., Kaminski S.
Polish Journal of Veterinary Sciences
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2016
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tom 19
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nr 3
The aim of this study was to evaluate the effect of C/T missense mutation (SOX5) gene on selected parameters of non-frozen semen in Polish Holstein – Friesian bulls. Three hundred and sixty eight bulls were genotyped by the PCR-Rsa I technique. Semen was collected and evaluated by standardized procedure used in an AI station. Significant associations were found between SOX5 genotypes and sperm concentrations (p=0.020) and sperm motility (p=0.014). Bulls with TT genotype had the lowest values of both traits. Our results demonstrate that C/T missense mutation within the SOX5 gene is involved in bull sperm quality.
2
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Missense mutation within cystic fibrosis transmembrane conductance regulator (CFTR) gene is associated with selected parameters of the frozen-thawed sperm in Holstein-Friesian bulls

81%
Kaminski S., Hering D. M., Kordan W., Lecewicz M.
Polish Journal of Veterinary Sciences
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2019
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tom 22
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nr 2
3
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Selected qualitative and biochemical parameters of cryopreserved semen of Holstein-Friesian (HF) AI bulls

71%
Lecewicz M., Hering D. M., Kaminski S., Majewska A., Kordan W.
Polish Journal of Veterinary Sciences
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2015
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tom 18
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nr 1
Selected qualitative and biochemical parameters were determined in cryopreserved semen used for artificial insemination, sampled from 120 bulls reared at the Animal Breeding and Insemination Center in Bydgoszcz. The total average motility of the analyzed sperm samples was determined at 62.51%. The percentage of motile spermatozoa displaying progressive forward motility was 21.65%. Analyzed samples were characterized by a high percentage of sperm cells with a intact plasma membrane (71.21%) and active mitochondria (71.32%). High efficiency of the enzymatic antioxidant system of the evaluated sperm cells was demonstrated by high activity of CAT, GPx and SOD (494.37, 2847.83 and 5.31U/1x109 spermatozoa, respectively) values and low values of the DNA Fragmentation Index (9.32). The results of the study, obtained with the involvement of advanced analytical methods, indicate a high fertilizing capability of the analyzed sperm samples.
4
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Influence of splicing mutation within the lysophosphatidic acid receptor 1 gene (LPAR1) on semen quality in Holstein-Friesian bulls

71%
Kaminski S., Hering D. M., Kordan W., Lecewicz M., Sazanov A.
Polish Journal of Veterinary Sciences
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2018
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tom 21
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nr 2
5
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Assessment of genomic inbreeding in Polish Konik horses

71%
Kaminski S., Hering D. M., Jaworski Z., Zabolewicz T., Rusc A.
Polish Journal of Veterinary Sciences
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2017
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tom 20
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nr 3
The aim of this study was to assess the inbreeding coefficient of Polish Konik horses based on runs of homozygosity (ROH). Ninety six horses kept in 6 herds located across Poland were genotyped with the use of EquineSNP60 BeadChip (Illumina). SNP markers with a Minor Allele Frequency lower than 0.01 and SNPs assigned to chromosome X or Y were excluded from the study. A total of 50 708 SNPs were included for statistical analysis (SVS software, Golden Helix). The analysis showed that the population is in genetic equilibrium, with Hₑ and Hₒ estimates both equal to 0.3086. Seven categories of Runs of Homozygozity (ROH) length were defined: >0.5, >1, >2, >4, >8, >16, >25 Mb. The genomic inbreeding coefficient derived from ROH (FROH) calculated for each ROH length ranged from 15.96% based on the shortest ROH (>0,5Mb) to 2.71% for the longest ROH (>25Mb). Among individual horses, the inbreeding coefficient ranged from 5.25% to 22.41% (for ROH >1Mb). Analysis of ROH in Polish Koniks allows for more effective management of their inbreeding in the future.
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The effect of insertion/deletion polymorphisms within the promoter and intron 1 sequences of the PRNP gene on the breeding value of Holstein-Friesian bulls

51%
Czarnik U., Strychalski J., Olenski K., Barcewicz M., Hering D. M., Puckowska P., Pierzchala M., Urbanski P., Pareek C. S.
Animal Science Papers and Reports
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2015
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tom 33
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nr 1
The aim of this study was to verify the hypothesis that insertion/deletion (indel) polymorphisms within the promoter and intron 1 sequences of the prion protein (PRNP) gene can affect the breeding value of Holstein-Friesian bulls. The experimental material included 261 Holstein-Friesian bulls born between 1997 – 2002. It was shown that the polymorphism in the intron 1 (12 bp indel) sequence had a more significant effect on the analyzed traits than a polymorphism in the promotor (23 bp indel) sequence. A deletion allele within intron 1 (12del) significantly increased the bulls’ breeding value for milk yield (p=0.001), protein yield (p=0.042), type and conformation (p=0.018),udder width (p=0.003), dairy character (p=0.004), and decreased days open (p=0.022). A deletion allele (23del) at the polymorphic locus of the promoter significantly decreased the bulls’ breeding value for milk yield (p=0.001), udder width (p=0.029) and dairy character (p=0.033). Analysis of both alleles showed that 23del-12del haplotype increased the bulls’ breeding value for udder traits:udder (p=0.008), fore udder (p=0.043) and udder depth (p=0.041), and decreased for fat kontent (p=0.047) and conformation traits - body depth (p<0.001), chest width (p=0.029) and rear leg set - side view (p=0.029). Also positive effect of 23del-12ins haplotype for days open (p=0.003) and days between calving and first insemination (p=0.045) was observed.
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