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Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

100%

Nekoei S., Taktaz Hafshejani T., Doosti A., Khamesipour F.

Polish Journal of Veterinary Sciences

2015

tom 18

nr 4

Bovine leukemia virus (BLV) is a deltaretrovirus which infects and induces proliferation of B-lymphocytes in the peripheral blood circulation and in lymphoid organs primarily of cattle, leading to leukemia/lymphoma. This study was carried out to investigate the presence of BLV in cattle, sheep and camels from the Chaharmahal va Bakhtiary and Isfahan provinces in Iran. A total of 874 blood samples collected from cattle, sheep and camels were used in this study to detect BLV using a nested- PCR. The results from this study indicated that 17.2% (n=874) of all blood samples collected were positive for BLV. The percentages of blood samples positive for BLV from cattle, sheep and camels were 22.1 (n=657), 5.3 (n=95) and 0 (n=122) respectively. The results from this study showed that BLV infected cattle and sheep. Camels seemed to be resistant to BLV infection. This study contributes to the nationwide effort to obtain baseline information on the prevalence of BLV, which will assist in planning the control strategy for the disease in Iran.

Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction

100%

Khamesipour F., Doosti A., Emadi M. F., Awosile B.

Bulletin of the Veterinary Institute in Puławy

2014

tom 58

nr 4

The study was conducted to detect Brucella sp. and Leptospira sp. in blood samples of dogs in Isfahan and Shahrekord province in Iran. A total of 94 blood samples were collected from dogs of different breed, age, sex, and dogs' type (stray or non- stray). The samples were examined using conventional polymerase chain reaction (PCR). Fourteen (14.89%) dogs were positive for Brucella sp. and 18 (19.15%). dogs for Leptospira sp. There were no significant differences between the prevalence of the pathogens, provinces, sex, and age groups (P > 0.05). However, there was a statistically significant difference in prevalence of Brucella sp. and Leptospira sp. between strav and non-stray dogs (P < 0.0001; χ² = 30.3767). The study also demonstrated that PCR was successfully used for the first time in Iran for the detection of Brucella sp. and Leptospira sp. in blood samples of dogs. Therefore, we recommend the PCR as a supplementary method with other commonly recognised methods (e.g. serological methods) for the diagnosis of subclinical infections with the microorganisms. Strict measures for the control of stray dogs are also highly recommended.

Ograniczanie wyników

1 Bulletin of the Veterinary Institute in Puławy

1 Polish Journal of Veterinary Sciences

2 Doosti A.

2 Khamesipour F.

1 Awosile B.

1 Emadi M. F.

1 Nekoei S.

1 Taktaz Hafshejani T.

1 2015

1 2014

Wyniki wyszukiwania

Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

Detection of Brucella sp. and Leptospira sp. in dogs using conventional polymerase chain reaction