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TCP family genes control leaf development and its responses to gibberellin in celery

100%

Duan A.-Q., Wang Y.-W., Feng K., Liu J.-X., Xu Z.-S., Xiong A.-S.

Acta Physiologiae Plantarum

2019

tom 41

nr 09

Celery is a biennial herb of the Apiaceae family and is a leafy vegetable crop widely cultivated worldwide. TCP (teosinte branched1/cycloidea/proliferating cell factors), a transcription factor family, is involved in cell growth and leaf tissue proliferation. In this study, 32 AgTCP transcription factors were identified and analyzed based on the celery transcriptome and genome database and divided into two classes. Analysis of structural feature, phylogenetic tree, interaction network, and subcellular localization of AgTCP proteins was performed. It is shown that the AgTCP2 protein was positioned in the nucleus, and TCP proteins in the same group had higher similarity. Heatmap clusters of AgTCP genes expression levels during different celery leaf developmental stages suggested that the genes from the same evolutionary branch tended to form a cluster. The expression profiles of four AgTCP genes were detected at celery leaf developmental stages and under gibberellin (GA₃) treatment. As a CYC-type gene, AgTCP4 showed significantly increased expression levels under developmental stage and GA₃ treatment in ‘Liuhe Huangxinqin’. The results of this work give potential helpful information for further analysis of the role of AgTCP transcription factors in leaf development and hormone regulation in celery.

Cloning and characterization of the genes encoding the small and large subunit of the ADP-glucose pyrophosphorylase in lotus (Nelumbo nucifera Gaertn)

84%

Cheng N., Zeng X.-F., Zheng X.-F., Diao Y., Wang Y.-W., Xie K.-Q., Zhou M.-Q., Hu Z.-L.

Acta Physiologiae Plantarum

2015

tom 37

nr 01

In higher plants, ADP-glucose pyrophosphorylase (EC 2.7.7.27, AGPase), a heterotetrameric enzyme comprised of two small and two large subunits, carries out the first committed step of starch synthesis. To characterize the AGPase genes in lotus, we cloned a small subunit and a large subunit of AGPase using the rapid amplification of cDNA ends (RACE) technique, designated NnAGPS1 and NnAGPL1 genes, respectively. The full-length cDNA of NnAGPS1 was 2,168 bp which codes for a protein of 524 amino acids and of NnAGPL1 was 2,304 bp encoding a protein of 528 amino acids. Complete genomic structures of NnAGPS1 (comprising 9 exons and 8 introns) and NnAGPL1 (comprising 14 exons and 13 introns) were distributed over 3.8 and 4.8 kb, separately. Phylogenetic tree divided the AGPase subunits into two classes: small subunit (SSU) and large subunit (LSU). Sequence alignment of these two genes with other plant species displayed a vast dissimilarity in the N-terminal regions. Results of quantitative real-time RT-PCR (qRT-PCR) showed that both genes were expressed in all tissues, including leaf, leafstalk, rhizome and root. The highest expression of NnAGPS1 was observed in leaves and NnAGPL1 seemed to be preferentially expressed in rhizomes. Besides, transcripts of NnAGPS1 decreased significantly while NnAGPL1 kept a steady level during the early to middle rhizome-swelling stages. Our study will provide important molecular information for the genetic modification and regulation of starch content in lotus.

Ograniczanie wyników

2 Acta Physiologiae Plantarum

2 Wang Y.-W.

1 Cheng N.

1 Diao Y.

1 Duan A.-Q.

1 Feng K.

1 Hu Z.-L.

1 Liu J.-X.

1 Xie K.-Q.

1 Xiong A.-S.

1 Xu Z.-S.

1 Zeng X.-F.

1 Zheng X.-F.

1 Zhou M.-Q.

1 2019

1 2015

Wyniki wyszukiwania

TCP family genes control leaf development and its responses to gibberellin in celery

Cloning and characterization of the genes encoding the small and large subunit of the ADP-glucose pyrophosphorylase in lotus (Nelumbo nucifera Gaertn)